Skip to main content
SpringerLink
Log in

Use of monoclonal antibodies for the identification of different antigenic domains in apple chlorotic leaf spot virus

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Thirteen monoclonal antibodies (Mabs) specific for apple chlorotic leaf spot virus (ACLSV), produced by the somatic cell hybridization technique, were used to investigate the antigenic structure of the virus. Epitope specificity studies showed that these Mabs defined in ACLSV particles seven independent antigenic domains, representing at least eight distinct epitopes. One of them was present only in virions and not in dissociated subunits. It appeared that the interaction between a Mab and the virus could, in some cases, induce conformational changes in the viral particles which enhanced the binding of others. Twenty nine virus isolates differing in geographical origin, primary hosts and symptomatology were tested with these monoclonal antibodies by ELISA. With the exception of two Mabs which did not react with three cherry isolates, and one Mab which did not react with one plum isolate, all of them recognized all ACLSV isolates tested.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Altschuh D, Al Moudallal Z, Briand JP, Van Regenmortel MVH (1985) Immunochemical studies of tobacco mosaic virus — VI. Attempts to localize viral epitopes with monoclonal antibodies. Mol Immunol 22: 329–337

    Google Scholar 

  2. Avrameas S (1969) Coupling of enzymes to proteins with Glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry 6: 43–52

    Google Scholar 

  3. Bar-Joseph M, Garsney SM, Gonsalves D (1979) The Closteroviruses: a distinct group of elongated plant viruses. Adv Virus Res 25: 93–168

    Google Scholar 

  4. Bernhard R, Dunez J (1971) Le virus du chlorotic leaf spot. Contamination de differentes espèces dePrunus. In: Populations de virus. VIIIéme symposium virus des arbres fruitiers. Ann Phytopathol 317–335

  5. Bruck C, Mathot S, Portelle D, Berte C, Franssen JD, Herion P, Burny A (1982) Monoclonal antibodies define eight independent antigenic regions on the bovine leukemia virus (BLV) envelope glycoprotein gp 51. Virology 122: 342–352

    Google Scholar 

  6. Chairez R, Lister RM (1973) Soluble antigens associated with the infection with apple chlorotic leaf spot virus. Virology 54: 506–514

    Google Scholar 

  7. Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34: 475–483

    Google Scholar 

  8. Dekker EL, Pinner MS, Markham PG, Van Regenmortel MHV (1988) Characterization of maize streak virus isolates from different plant species by polyclonal and monoclonal antibodies. J Gen Virol 69: 983–990

    Google Scholar 

  9. Detienne G, Delbos R, Dunez J (1980) Use and versatility of the immunoenzymatic ELISA procedure in the detection of different strains of an apple chlorotic leaf spot virus. Acta Phytopathol Acad Sci Hung 15: 39–45

    Google Scholar 

  10. Dietzgen RG (1986) Characterization of antigenic structure on Arabis mosaic virus with monoclonal antibodies. Arch Virol 91: 163–173

    Google Scholar 

  11. Dore I, Weiss E, Altschuh D, Van Regenmortel MHV (1988) Visualization by electron microscopy of the location of tobacco mosaic virus epitopes reacting with monoclonal antibodies in enzyme immunoassay. Virology 162: 279–290

    Google Scholar 

  12. Dougherty WG, Willis L, Johnston RE (1985) Topographic analysis of tobacco etch capsid protein epitopes. Virology 144: 66–72

    Google Scholar 

  13. Dunez J, Marenaud C, Delbos R, Lansac M (1972) Variability of symptoms induced by the apple chlorotic leafspot (CLSV). A type of CLSV probably responsible for Bark Split disease of prune trees. Plant Dis Rep 56: 293–295

    Google Scholar 

  14. Dunez J, Delbos R, Bertranet R (1973) Le rôle de differents facteurs de stabilisation appliqués à la purification du virus du chlorotic leaf spot du pommier (CLSV). Ann Phytopathol 5: 255–264

    Google Scholar 

  15. Ey PL, Prowse SJ, Jenkin CR (1978) Isolation of pure IgG 1, IgG 2 a and IgG 2 b immunoglobulins from mouse serum using Protein A-Sepharose. Immunochemistry 15: 4529–436

    Google Scholar 

  16. Guesdon JL, Ternynck T, Avrameas S (1979) The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27: 1131–1139

    Google Scholar 

  17. Huguenot C, Givord L, Sommermeyer G, Van Regenmortel MHV (1989) Differentiation of peanut clump virus serotypes by monoclonal antibodies. Res Virol 140: 87–102

    Google Scholar 

  18. Kerlan C, Mille B, Dunez J (1981) Immunosorbent electron microscopy for detecting apple chlorotic leaf spot and plum pox viruses. Phytopathology 71: 400–404

    Google Scholar 

  19. Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibodies of predefined specificity. Nature 256: 495–497

    Google Scholar 

  20. Lubeck M, Gerhard W (1982) Conformational changes at topologically distinct antigenic sites on the influenza A/PR/34 virus HA molecule are induced by the binding of monoclonal antibodies. Virology 118: 1–7

    Google Scholar 

  21. Lussenhop NO, Goertz R, Wabuke-Bunoti M, Gehrz R, Kari B (1988) Epitope analysis of human cytomegalovirus glycoprotein complexes using murine monoclonal antibodies. Virology 164: 362–372

    Google Scholar 

  22. Pena-Iglesias A, Ayuso P (1973) Preliminary identification of the viruses producing Spanish apricot pseudopox (viruela) and apricot mosaic diseases. Acta Horticult 44: 255–265

    Google Scholar 

  23. Poul F, Dunez J (1989) Production and use of monoclonal antibodies for the detection of apple chlorotic leaf spot virus. J Virol Methods 25: 153–166

    Google Scholar 

  24. Ragozzino A, Pugliano G (1974) La butteratura delle albicocche. Indagini preliminari sulla eziologia. Riv Ortoflorfrutt Ital 58: 136–145

    Google Scholar 

  25. Sober J, Jarvekulg L, Toots I, Radavsky J, Villems R, Saarma M (1988) Antigenic characterization of potato virus X with monoclonal antibodies. J Gen Virol 69: 1799–1807

    Google Scholar 

  26. Steward MW, Lew AM (1985) The importance of antibody affinity in the performance of immunoassays for antibody. J Immunol Methods 78: 173–190

    Google Scholar 

  27. Stone MR, Nowinski RC (1980) Topological mapping of murine leukemia virus proteins by competition-binding assays with monoclonal antibodies. Virology 100: 370–381

    Google Scholar 

  28. Thomas JE, Massalski PR, Harrison BD (1986) Production of monoclonal antibodies to African cassava mosaic virus and their differences in their reactivities with other whitefly-transmitted geminivirus. J Gen Virol 67: 2739–2748

    Google Scholar 

  29. Torrance L, Larkins AP, Butcher GW (1986) Characterization of monoclonal antibodies against potato virus X and comparison of serotypes with resistances group. J Gen Virol 67: 57–67

    Google Scholar 

  30. Underwood PA (1988) Measurement of the affinity of antiviral antibodies. Adv Virus Res 34: 283–309

    Google Scholar 

  31. Van Regenmortel MHV (1982) Serology and immunochemistry of plant viruses. Academic Press, New York

    Google Scholar 

  32. Vela C, Cambra M, Cortes E, Moreno P, Miguet JG, Perez De San Roman C, Sanz A (1986) Production of monoclonal antibodies specific for citrus tristeza virus and their use for diagnosis. J Gen Virol 67: 91–96

    Google Scholar 

  33. Zrein M, Burckard J, Van Regenmortel MHV (1986) Use of the biotin-avidin system for detecting a broad range of serologically related plant viruses by ELISA. J Virol Methods 13: 121–128

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut National de la Recherche Agronomique, Centre de Recherche de Bordeaux, Station de Pathologie Végétale, Villenave d'Ornon, France

    F. Poul & J. Dunez

Authors
  1. F. Poul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Dunez
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Poul, F., Dunez, J. Use of monoclonal antibodies for the identification of different antigenic domains in apple chlorotic leaf spot virus. Archives of Virology 114, 191–202 (1990). https://doi.org/10.1007/BF01310748

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01310748

Keywords

Search

Navigation