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Modified oligopeptides designed to interact with the HIV-1 proteinase inhibit viral replication

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Summary

The human immunodeficiency virus 1 (HIV-1) codes for a proteinase that cuts viral proteins at specific sites. We have tested 13 modified oligopeptides related to these cleavage sites to see if they inhibit viral replication. To indicate whether a decrease in replication could be due to a general inhibition of cell metabolism, we also measured the effect of the peptides on cellular protein synthesis. Three of the peptides tested (Ac-Gln-Asn-Sta-Val-NH2, Ac-Gln-Asn-Sta-Val-Val-NH2, and Ac-Glu-Asn-Sta-Ile-NH2) inhibited HIV-1 replication at concentrations that did not inhibit protein synthesis. Ac-Gln-Asn-Sta-Val-NH2 was the most potent, causing an approximately 40% decrease in viral replication, measured as the synthesis of HIV-1 antigens and the formation of infectious particles.

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  1. Department of Virology, National Institute of Public Health, Oslo, Norway

    B. Grinde, O. Hungnes & E. Tjøtta

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  1. B. Grinde
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  2. O. Hungnes
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  3. E. Tjøtta
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Grinde, B., Hungnes, O. & Tjøtta, E. Modified oligopeptides designed to interact with the HIV-1 proteinase inhibit viral replication. Archives of Virology 114, 167–173 (1990). https://doi.org/10.1007/BF01310746

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  • DOI: https://doi.org/10.1007/BF01310746

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