Skip to main content
SpringerLink
Log in

Association of non-viral proteins with recombinant vaccinia virus virions

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Evidence is presented which suggests that recombinant vaccinia virus particles (VV : CAT), containing the bacterial chloramphenicol acetyl transferase gene, are capable of encapsidating both the foreign protein which they encode (CAT) as well as cellular enzymes such as thymidine kinase. These results are discussed with respect to using VV to passively introduce biologically-active proteins into cells or organisms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Brown F, Schild GC, Ada GL (1986) Recombinant vaccinia viruses as vaccines. Nature 319: 549–550

    Google Scholar 

  2. Cochran MA, Puckett C, Moss B (1985)In vitro mutagenesis of the promoter region for a vaccinia virus gene: evidence for tandem early and late regulatory signals. J Virol 54: 30–37

    Google Scholar 

  3. Gorman CM, Moffat LF, Howard BH (1982) Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol 2: 1044–1051

    Google Scholar 

  4. Hruby DE, Ball LA (1981) Cell-free synthesis of enzymatically active vaccinia virus thymidine kinase. Virology 113: 594–601

    Google Scholar 

  5. Hruby DE, Guarino LA, Kates JR (1979) Vaccinia virus: requirement for the host cell nucleus. J Virol 29: 705–715

    Google Scholar 

  6. Morrison DK, Moyer RW (1986) Detection of a subunit of cellular pol II within highly purified preparations of RNA polymerase isolated from rabbit poxvirus virions. Cell 44: 587–596

    Google Scholar 

  7. Moss B (1968) Inhibition of Hela cell protein synthesis by the vaccinia virion. J Virol 2: 1028–1037

    Google Scholar 

  8. Paoletti E, Perkins ME, Piccini A (1985) Live recombinant vaccines using gentically engineered vaccinia virus. Antiviral Res 1: 301–307

    Google Scholar 

  9. Rice CM, Franke CA, Strauss JA, Hruby DE (1985) Expression of Sindbis virus structural proteins via recombinant vaccinia virus: synthesis, processing, and incorporation into mature sindbis virions. J Virol 56: 227–239

    Google Scholar 

  10. Rohrman G, Moss B (1985) Transcription of vaccinia virus early genes by a template-dependent soluble extract of purified virions. J Virol 56: 349–355

    Google Scholar 

  11. Smith GL, Moss B (1984) Vaccinia virus expression vectors: construction, properties, and applications. Biotechniques 1: 306–312

    Google Scholar 

  12. Thomas G, Herbert E, Hruby DE (1986) Expression and cell type-specific processing of human preproenkephalin with a vaccinia recombinant. Science 232: 1641–1643

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center for Gene Research, Department of Microbiology, Oregon State University, Corvallis, Oregon, USA

    Christine A. Franke & D. E. Hruby

Authors
  1. Christine A. Franke
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. E. Hruby
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 1 Figure

Rights and permissions

Reprints and permissions

About this article

Cite this article

Franke, C.A., Hruby, D.E. Association of non-viral proteins with recombinant vaccinia virus virions. Archives of Virology 94, 347–351 (1987). https://doi.org/10.1007/BF01310729

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01310729

Keywords

Search

Navigation