Skip to main content
SpringerLink
Log in

Monoclonal antibodies define antigenic regions on the major internal protein p24 of bovine leukemia virus (BLV)

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

The major core protein p24 of bovine leukemia virus (BLV) was characterized by ten monoclonal antibodies. Competitive binding assays were performed in order to analyze the topography of the antigenic determinants by enzyme-linked immunosorbent assay. At least three independent antigenic regions were demonstrated on the BLV p24 molecule.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Aida Y, Onuma M, Mikami T, Izawa H (1985) Topographical analysis of tumor-associated antigens on bovine leukemia virus-induced bovine lymphosarcoma. Cancer Res 45: 1181–1186

    Google Scholar 

  2. Aida Y, Onuma M, Ogawa Y, Mikami T, Izawa H (1985) Tumor-associated antigens on bovine leukemia virus-induced bovine lymphosarcoma identified by monoclonal antibodies. Cancer Res 45: 1174–1180

    Google Scholar 

  3. Avrameas S (1969) Coupling of enzymes to proteins with glutaraldehyde. Immunochemistry 6: 43–52

    Google Scholar 

  4. Bruck C, Mathot S, Portetelle D, Berte C, Franssen JD, Herion P, Burny A (1982) Monoclonal antibodies define eight independent antigenic regions on the bovine leukemia virus (BLV) envelope glycoprotein gp51. Virology 122: 342–352

    Google Scholar 

  5. Boyüm A (1968) Isolation of leukocytes from human blood, further observations. Methylcellulose, dextran, and ficoll as erythrocyte aggregating agent. Scand J Clin Lab Invest 21: 31–50

    Google Scholar 

  6. Burny A, Bruck C, Chantrenne H, Cleuter Y, Dekegel D, Ghysdael J, Kettmann R, Leclercq M, Leunen J, Mammerickx M, Portetelle D (1980) Viral oncology. In:Klein G (ed) Bovine leukemia virus: molecular biology and epidemiology. Raven Press, New York, pp 231–289

    Google Scholar 

  7. Onoue K, Yagi Y, Pressman D (1964) Multiplicity of antibody proteins in rabbit anti-α-azobenzenearsonate sera. J Immunol 92: 173–184

    Google Scholar 

  8. Onuma M, Olson C (1977) Tumor-associated antigen in bovine and ovine lymphosarcoma. Cancer Res 37: 3249–3256

    Google Scholar 

  9. Onuma M, Olson C, Driscoll DM (1976) Properties of two isolated antigens associated with bovine leukemia virus infection. J Natl Cancer Inst 57: 571–578

    Google Scholar 

  10. Oroszlan S, Copeland TD, Kalyanaraman VS, Sarngardharan MG, Schultz AM, Gallo RC (1984) Human T-cell leukemia/lymphoma virus. In:Gallo RC, Essex ME, Gross L (eds) Chemical analysis of human T-cell leukemia virus structural proteins. Cold Spring Harbor Lab, pp 101–110

  11. Oroszlan S, Sarngadharan MG, Gopeland TD, Kalranaraman VS, Gilden RV, Gallo RC (1982) Primary structure analysis of the major internal protein p 24 of human type C T-cell leukemia virus. Proc Natl Acad Sci USA 79: 1291–1294

    Google Scholar 

  12. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheet: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354

    Google Scholar 

  13. Van der Maaten MJ, Miller JM (1976) Replication of bovine leukemia virus in monolayer cell culture. Bibl Haematol 43: 360–362

    Google Scholar 

Download references

Author information

Author notes

  1. Y. Aida

    Present address: Laboratory of Gene Technology and Safety, Tsukuba Life Science Center, Institute of Physical and Chemical Research, Yatabe-cho, Tsukuba, Ibaraki, Japan

Authors and Affiliations

  1. Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan

    Y. Aida, K. Tsukiyama, Y. Ogawa, T. Fujieda, T. Mikami & H. Izawa

  2. Department of Veterinary Microbiology, College of Dairy Agriculture, Bunkyodai, Ebetsu, Japan

    M. Onuma

Authors
  1. Y. Aida
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Onuma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Tsukiyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Ogawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Fujieda
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. Mikami
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. H. Izawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 2 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aida, Y., Onuma, M., Tsukiyama, K. et al. Monoclonal antibodies define antigenic regions on the major internal protein p24 of bovine leukemia virus (BLV). Archives of Virology 94, 315–321 (1987). https://doi.org/10.1007/BF01310725

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01310725

Keywords

Search

Navigation