Summary
Biochemical and immunological properties of retrovirus-D/New England (here referred as R-D/NE) recently isolated at the New England Regional Primate Research Center, Southborough, Ma, from a rhesus monkey with acquired immune deficiency syndrome were investigated and compared to the prototype type D retroviruses Mason-Pfizer monkey virus (MPMV) and permanent human fibroblast virus (PMFV) isolated from a breast carcinoma of a rhesus monkey and a continuous human cell line, respectively. The polypeptide composition of R-D/NE propagated in a human lymphoid B cell line (Raji cells) has been investigated using SDS-polyacrylamide gel electrophoresis. Staining with Coomassie blue and labelling with14C amino acids revealed seven viral polypeptides with molecular weights of 4,000, 10,000, 12,000, 15,000, 18,000, 27,000, and 80,000 Da which were also shared by MPMV and PMFV. The 80,000 Da protein was shown to be a glycoprotein by incorporation of3H glucosamine. The 18,000 Da protein was identified as a phosphoprotein of R-D/NE. p 18 structural proteins of MPMV and PMFV represent phosphoproteins of their respective viruses as well. All three phosphorylated proteins contain O-phosphoserine as major phosphoamino acid. The comparison of tryptic peptide maps of the major internal structural proteins of R-D/NE, MPMV, and PMFV revealed a striking similarity among p 10/p 12 and p 15. proteins. A minor difference was detected among the tryptic peptide digests of p 4 and p 27 proteins. Antiserum against p 15 of MPMV showed a significantly weaker binding to R-D/NE than to MPMV and PMFV at high dilutions. compared to our earlier findings of a gp 70 might be due to the different host cell lines used for virus propagation.
Summarizing our data, R-D/NE seems to resemble in many properties more PMFV, the type D retrovirus isolated from a permanent human cell line, than MPMV. This is supported by our recent results obtained by restriction endonuclease site analysis of covalently closed circular DNA of R-D/NE, PMFV and MPMV (Virology, in press).
Similar content being viewed by others
References
Benveniste RE, Jodaro GJ (1977) Evolution of primate oncornaviruses: an endogenous virus from languns(Presbytis spp.) with related virogene sequences in Old World monkeys. Proc Natl Acad Sci USA 74: 4557–4561
Bonner WM, Lasky RA (1974) A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem 46: 83–88
Bradac J, Hunter E (1984) Polypeptides of Mason-Pfizer monkey virus. I. Synthesis and processing of the gag-gene products. Virology 138: 260–275
Chang JY, Brauer D, Wittmann-Liebold B (1978) Microsequence analysis of peptides and proteins using 4-N,N-dimethyl-aminoazobenzene-4'iothiocyanate/phenylisothiocyanate double coupling method. FEBS Lett. 93: 205–214
Daniel MD, King NW, Letvin NL, Hunt RD, Sehgal PK, Desrosiers RC (1984) A new type D retrovirus isolated from macaques with an immunodeficiency syndrome. Science 223: 602–605
Fine D, Schochetman G (1978) Type-D primate retroviruses: a review. Cancer Res 38: 3123–3139
Gelderblom H, Bauer H, Ogura H, Wigand R, Fischer AB (1974) Oncornavirus-like particles in HeLa cells. I. Fine structure and comparative morphological classification. Int J Cancer 13: 2460–2530
Graffi A, Bierwolf D, Widmaier R, Bender E, Wunderlich V, Rudolph M, Mothes E, Niezabitowski A, Papsdorf G (1974) In der Gewebekultur züchtbares Oncorna-Virus in malignen permanenten menschlichen Zellinien vom Embryo einer krebskranken Frau. Dtsch Ges Wesen 29: 1489–1498
Heberling RL, Barker ST, Kalter SS, Smith GC, Helmke RJ (1977) Isolation of an oncornavirus from a squirrel monkey(saimiri sciureus) lung culture. Science 195: 289–292
Henderson LE, Sowder R, Smythers G, Benveniste RE, Oroszlan S (1985) Purification and N-terminal amino acid sequence comparisons of structural proteins from retrovirus-D/Washington and Mason-Pfizer monkey virus. J Virol 55: 778–787
Ilyin KV, Bykovsky AF, Zhdanov VM (1973) An oncornavirus isolated from human cancer cell line. Cancer 37: 89–96
Jensen EM, Zelljadt I, Chopra HC, Mason MM (1970) Isolation and propagation of a virus from a spontaneous mammary carcinoma of a rhesus monkey. Cancer Res 30: 2388–2393
Lämmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680–685
Letvin NL, Eaton KA, Aldrich WR, Sehgal PK, Blake BJ, Schlossmann SF, King NW, Hunt RD (1984) Experimental infection of rhesus monkeys with type D retrovirus. J Virol 52: 683–686
Marx PA, Maul DH, Osborn KG, Lerche NW, Moody P, Lowenstine LJ, Henrickson RV, Arthur LO, Gravell M, London WT, Sever JL, Levy JA, Munn RJ, Gardner MB (1984) Simian AIDS: isolation of a type D retrovirus and disease transmission. Science 223: 1083–1086
Marx PA, Bryant ML, Osborn KG, Maul DH, Lerche NW, Lowenstine LJ, Kluge JD, Zaiss CP, Henrickson RV, Shiigi SM, Wilson BJ, Malley A, Olson LC, McNulty WP, Arthur LO, Gilden RV, Barker CS, Hunter E, Munn RJ, Heidecker G, Gardner MB (1985) Isolation of a new serotype of simian acquired immune deficiency syndrome type D retrovirus from Celebes black macaques(Macaca nigra) with immune deficiency and retroperitoneal fibromatosis. J Virol 56: 571–578
Morozov VA (1982) Polypeptide composition and gag gene-coded products of type D oncovirus from HEp 2 cells. Neoplasma 29: 369–375
Power MD, Marx PA, Bryant ML, Gardner MB, Barr, PJ, Luciw PA (1985) Nucleotide sequence of SRV-1, a type D simian acquired immune deficiency syndrome retrovirus. Science 231: 1567–1572
Salacinski PR, McLean C, Sykes JE, Clement-Jones VV, Lowry PJ (1981) Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3α, 6α-diphenyl glycouril (Iodogen). Anal Biochem 117: 136–146
Schochetman G, Kortright K, Schlom J (1975) Mason-Pfizer monkey virus: analysis and localization of virion proteins and glycoproteins. J Virol 16: 1208–1219
Sen A, Sherr CJ, Todaro GJ (1977) Phosphorylation of murine type C viral protein p 12 proteins regulates their extent of binding to the homologous viral RNA. Cell 10: 489–496
Sonigo P, Barker C, Hunter E, Wain-Hobson S (1986) Nucleotide sequence of Mason-Pfizer monkey virus: an immunosuppressive D-type retrovirus. Cell 45: 375–385
Stromberg K, Benveniste RE, Arthur LO, Rabin H, Gidden WE, Ochs HD, Morton WR, Tsai C (1984) Characterization of exogenous type D retrovirus from a fibroma of a macaque with simian AIDS and fibromatosis. Science 224: 289–292
Todaro GJ, Benveniste RE, Sherr CJ, Schlom J, Schidlovsky G, Stephenson JR (1978) Isolation and characterization of a new type D retrovirus from the Asian primate,Presbytis obscurus (spectacled langur). Virology 84: 189–194
Uckert W, Wunderlich V, Bender E, Sydow G, Bierwolf D (1980) The protein pattern of PMF virus, a type D retrovirus from malignant permanent human cell lines. Arch Virol 64: 155–166
Uckert W, Hertling I, Denner J (1984) Further evidence from tryptic peptide analysis for heterogeneity among type D retroviruses. Arch Virol 81: 121–138
Uckert W, Hertling I, Kraft R, Bossmann H, Rössler H, Meyer U (1986) Structural components of bovine leukemia virus: further biochemical and immunological characterization of major structural proteins and glycoproteins. Virus Res 4: 343–356
Wunderlich V, Uckert W (1981) Type D retroviruses from human cells do not contain a minor glycoprotein shared by Old World monkey type D viruses. Arch Virol 70: 43–53
Author information
Authors and Affiliations
Additional information
With 8 Figures
Rights and permissions
About this article
Cite this article
Uckert, W., Wunderlich, V., Fiebach, H. et al. Biochemical and immunological characterization of structural proteins from retrovirus-D/New England and comparison to Mason-Pfizer monkey virus and permanent human fibroblast virus. Archives of Virology 94, 267–282 (1987). https://doi.org/10.1007/BF01310719
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01310719