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Dengue-2 virus infection of human mononuclear cell lines and establishment of persistent infections
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  • Original Papers
  • Published: March 1990

Dengue-2 virus infection of human mononuclear cell lines and establishment of persistent infections

  • I. Kurane1,
  • U. Kontny1,
  • J. Janus1 &
  • …
  • F. A. Ennis1 

Archives of Virology volume 110, pages 91–101 (1990)Cite this article

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Summary

Twenty three human mononuclear cell lines including ten myelomonocytic cell lines, eight B cell lines and five T cell lines, were examined to determine whether they could be infected with dengue-2 virus. All the cell lines were infected with dengue-2 virus as determined by immunofluorescent staining and by virus titration of culture supernatant fluids. K 562, Jiyoye and Jurkat, respectively, showed the highest percentage of infected cells of these myelomonocytic, B and T cell lines. Antibody to dengue-2 virus at subneutralizing concentrations augmented dengue-2 virus infection of myelomonocytic cell lines, but not of B cell lines or of T cell lines.

Persistent dengue-2 virus infection was established using a myelomonocytic cell line (K562), a B cell line (Raji), and a T cell line (HSB-2). These cell lines maintained a high percentage (more than 70%) of dengue-2 virus antigen-positive cells for at least 25 weeks. Very low titers of infectious dengue-2 virus were detected in the culture supernatant fluids of the persistently infected cells. Dengue-2 virus antigen-positive Raji cell clones were established from persistently-infected Raji cells using limiting dilutions and all of the cells in these clones were dengue-2 virus antigen-positive. These findings demonstrate that a variety of human mononuclear cell lines can be infected with dengue-2 virus and may be useful as models for the analysis of dengue virus-human cell interactions in dengue virus infections.

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Authors and Affiliations

  1. Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts, U.S.A.

    I. Kurane, U. Kontny, J. Janus & F. A. Ennis

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  1. I. Kurane
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  2. U. Kontny
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  3. J. Janus
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Kurane, I., Kontny, U., Janus, J. et al. Dengue-2 virus infection of human mononuclear cell lines and establishment of persistent infections. Archives of Virology 110, 91–101 (1990). https://doi.org/10.1007/BF01310705

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  • Received: 26 July 1989

  • Accepted: 19 September 1989

  • Issue Date: March 1990

  • DOI: https://doi.org/10.1007/BF01310705

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Keywords

  • Infectious Disease
  • Virus Infection
  • Infected Cell
  • Immunofluorescent Staining
  • Cell Clone
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