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Gallid herpesvirus 1 (infectious laryngotracheitis virus): cloning and physical maps of the SA-2 strain


Clones representing 90% of the genome of Gallid herpesvirus 1 (infectious laryngotracheitis virus; ILTV) were obtained and used in hybridization experiments to constructEcoRI,KpnI amdSmaI physical maps. The genome was 155 kilobase pairs (kbp) and comprised of a long unique sequence (120 kbp) and a short unique sequence (17 kbp) bounded by repeat sequences each of 9 kbp. An unrelated second pair of repeat sequences was located at 0.67 and 0.88 map untis. A terminal repeat of the unique long region (UL) was also detected, but no isomerization of UL was detected.

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  1. 1.

    Bagust TJ, Calnek BW, Fahey KJ (1986) Gallid-1 herpesvirus infection in the chicken. 3. Reinvestigation of the pathogenesis of infectious laryngotracheitis in acute and early post-acute respiratory disease. Avian Dis 30: 179–190

    Google Scholar 

  2. 2.

    Ben-Porat T, Rixon FJ, Blankenship ML (1979) Analysis of the structure of the genome of pseudorabies virus. Virology 95: 285–294

    Google Scholar 

  3. 3.

    Browning GF, Studdert MJ (1989) Physical mapping of a genome of equine herpesvirus 2 (equine cytomegalovirus). Arch Virol 104: 77–86

    Google Scholar 

  4. 4.

    Buckmaster AE, Scott SD, Sanderson MJ, Boursnell MEG, Ross NLJ, Binns MM (1988) Gene sequence and mapping data from Marek's disease virus and herpesvirus of turkeys: implications for herpesvirus classification. J Gen Virol 69: 2033–2042

    Google Scholar 

  5. 5.

    Collins J, Hohn B (1978) Cosmids: type of plasmid gene cloning vector that is pack-ageable in vitro in bacteriophage lambda heads. Proc Natl Acad Sci USA 75: 4242

    Google Scholar 

  6. 6.

    Denhardt DT (1966) A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23: 641–646

    Google Scholar 

  7. 7.

    Ecker JR, Hyman RW (1982) Varicella zoster virus DNA exists as two isomers. Proc Natl Acad Sci USA 79: 156–160

    Google Scholar 

  8. 8.

    Fahey KJ, Bagust TJ, York JJ (1983) Laryngotracheitis herpesvirus infection in the chicken: the role of humoral antibody in immunity to a graded challenge infection. Avian Pathol 12: 505–514

    Google Scholar 

  9. 9.

    Fukuchi K, Sudo M, Lee Y-S, Tanaka A, Nonoyama M (1984) Structure of Marek's disease virus DNA: detailed restriction enzyme map. J Virol 51: 102–109

    Google Scholar 

  10. 10.

    Griffin AM (1989) Identification of 21 genes of infectious laryngotracheitis virus using random sequencing of genomic DNA. J Gen Virol 70: 3085–3089

    Google Scholar 

  11. 11.

    Hanson LE (1984) Laryngotracheitis. In: Hofstad MS, Barnes HJ, Calnek BW, Reid WM, Yoder HW Jr (eds) Diseases of poultry, 8th edn. Iowa State University Press, Ames, IA, pp 444–451

    Google Scholar 

  12. 12.

    Henry BE, Robinson RA, Dauenhauer SA, Atherton SS, Hayward GS, O'Callaghan DJ (1981) Structure of the genome of equine herpesvirus type 1. Virology 115: 97–114

    Google Scholar 

  13. 13.

    Hohn B, Collins J (1980) A small cosmid for efficient cloning of large DNA fragments. Gene 11: 291

    Google Scholar 

  14. 14.

    Igarashi T, Takahashi M, Donovan J, Jessip J, Smith M, Hirai K, Tanaka A, Nonoyama M (1987) Restriction enzyme map of herpesvirus of turkey DNA and its colinear relationship with Marek's diseases virus DNA. Virology 157: 351–358

    Google Scholar 

  15. 15.

    Ish-Horowicz D, Burke JF (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9: 2989

    Google Scholar 

  16. 16.

    Jordan FTW (1981) Immunity to infectious laryngotracheitis. In: Rose ME, Payne LN, Freeman BM (eds) Avian immunology. British Poultry Science, Edinburgh, pp 245–254

    Google Scholar 

  17. 17.

    Kotiw M, Wilks CR, May JT (1982) Differentiation of infectious laryngotracheitis virus strains using restriction endonucleases. Avian Dis 26: 718–731

    Google Scholar 

  18. 18.

    Leib DA, Bradbury JM, Gaskell RM, Hughes CS, Jones RC (1986) Restriction endonuclease patterns of some European and American isolates of avian infectious laryngotracheitis virus. Avian Dis 30: 835–837

    Google Scholar 

  19. 19.

    Mayfield JE, Good PJ, VanOort HJ, Campbell AR, Reed DE (1983) Cloning and cleavage site mapping of DNS from bovine herpesvirus 1 (Cooper strain). J Virol 47: 259–264

    Google Scholar 

  20. 20.

    Odagiri Y (1982) Experimental pathology of infectious laryngotracheitis of poultry. Bull Univ Osaka Prefect [B] 34: 75–131

    Google Scholar 

  21. 21.

    Purcell DA, McFerran JB (1969) Influence of method of infection on the pathogenesis of infectious laryngotracheitis. J Comp Pathol 79: 285–291

    Google Scholar 

  22. 22.

    Robertson GM, Egerton JR (1981) Replication of infectious laryngotracheitis virus in chickens following vaccination. Aust Vet J 57: 119–123

    Google Scholar 

  23. 23.

    Roizman B (1982) The familyHerpesviridae: general description, taxonomy, and classification. In: Roizman B (ed) The herpesviruses, vol 1. Plenum, New York, pp 1–23

    Google Scholar 

  24. 24.

    Roizman B, Carmichael LE, Deinhardt F, de-The G, Nahmias AJ, Plowright W, Rapp F, Sheldrick P, Takahashi M, Wolf K (1981)Herpesviridae. Definition, provisional nomenclature, and taxonomy. Intervirology 16: 201–217

    Google Scholar 

  25. 25.

    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  26. 26.

    Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

    Google Scholar 

  27. 27.

    Straus SE, Aulakh HS, Ruyechan WT, Hay J, Casey TA, Vande Woude GF, Owens J, Smith HA (1981) Structure of varicella-zoster virus DNA. J Virol 40: 516–525

    Google Scholar 

  28. 28.

    Sullivan DC, Atherton SS, Staczek J, O'Callaghan DJ (1984) Structure of the genome of equine herpesvirus type 3. Virology 132: 352–367

    Google Scholar 

  29. 29.

    Whalley JM, Robertson GR, Davison AJ (1981) Analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleasesEcoRI,BglII andBamHI. J Gen Virol 57: 307–323

    Google Scholar 

  30. 30.

    Wilkie NM (1973) The synthesis and substructure of herpesvirus DNA: the distribution of alkali-labile single strand interruptions in HSV-1 DNA. J Gen Virol 21: 453–467

    Google Scholar 

  31. 31.

    Yanisch-Perron C, Vieira J, Messing J (1985) Improved M 13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC 19 vectors. Gene 33: 103–119

    Google Scholar 

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Johnson, M.A., Prideaux, C.T., Kongsuwan, K. et al. Gallid herpesvirus 1 (infectious laryngotracheitis virus): cloning and physical maps of the SA-2 strain. Archives of Virology 119, 181–198 (1991).

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  • Infectious Disease
  • Repeat Sequence
  • Unique Sequence
  • Terminal Repeat
  • Hybridization Experiment