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Serological characterisation of human rotaviruses propagated in cell cultures

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Summary

Fourteen strains of human rotaviruses were propagated in MA 104 cell culture using serum-free medium containing 10 µg/ml trypsin. Eleven of the strains were provided by laboratories in Japan and the U.S.A., and the remaining three from our own laboratory. The serological relationships between these strains were determined by rotavirus subgroup-specific enzyme-immunoassays (ELISA) and neutralisation of infectivity with serotype-specific antisera. All the isolates clearly belonged to one of two subgroups, but four distinct serotypes were represented. Furthermore, the recently reported fourth serotype (ST4) and two isolates from Japan (Hochi and Hosokawa) appeared to be related to a South American rotavirus strain reported in 1978 as a probable fourth serotype.

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References

  1. Beards, G. M., Pilfold, J. N., Thouless, M. E., Flewett, T. H.: Rotavirus serotypes by serum neutralisation. J. Med. Virol.5, 231–237 (1980).

    Google Scholar 

  2. Drosdov, S. G., Shekoyan, L. A., Korolev, M. B., Andzhaparidoze, A. G.: Human rotaviruses in cell culture: isolation and passaging. Voprosy Virusologii4, 389–392 (1979). (In Russian.)

    Google Scholar 

  3. Flewett, T. H., Thouless, M. E., Pilfold, J. N., Bryden, A. S., Candeias, J. A. N.: More serotypes of human rotaviruses. Lancetii, 632 (1978).

    Google Scholar 

  4. Greenberg, H. B., Kalica, A. R., Wyatt, R. G., Jones, R. W., Kapikian, A. Z., Chanock, R. M.: Rescue of non-cultivable human rotavirus by gene reassortment during mixed infection with Ts mutants of a cultivable bovine rotavirus. PNAS78, 420–424 (1981).

    Google Scholar 

  5. Greenberg, H. B., Wyatt, R. G., Kapikian, A. Z., Kalica, A. R., Flores, J., Jones, R. W.: Rescue and serotypic characterisation of non-cultivable human rotavirus by gene reassortment. Infect. Immun.37, 104–109 (1982).

    Google Scholar 

  6. Greenberg, H. B., McAuliffe, V., Valdesuso, J., Wyatt, R. G., Flores, J., Kalica, A. R., Hoshino, Y., Singh, N.: Serological analysis of the subgroup protein of rotavirus using monoclonal antibodies. Infect. Immun.39, 91–99 (1983).

    Google Scholar 

  7. Killen, H. M., Dimmock, N. J.: Identification of a neutralisation-specific antigen of a calf rotavirus. J. gen. Virol.62, 297–311 (1982).

    Google Scholar 

  8. Sato, K., Inaba, Y., Shinozuka, T., Matsumoto, M.: Isolation of human rotavirus in cell cultures. Arch. Virol.69, 155–160 (1981).

    Google Scholar 

  9. Sato, K., Inaba, Y., Miura, Y., Tokunisa, S., Matsumoto, M.: Antigenic relationships between rotaviruses from different species as studied by neutralisation and immunofluorescence. Arch. Virol.73, 45–50 (1982).

    Google Scholar 

  10. Thouless, M. E., Bryden, A. S., Flewett, T. H.: Serotypes of human rotavirus. Lanceti, 39 (1978).

    Google Scholar 

  11. Thouless, M. E., Beards, G. M., Flewett, T. H.: Serotyping and subgrouping of rotavirus strains by the ELISA test. Arch. Virol.73, 219–230 (1982).

    Google Scholar 

  12. Urasawa, T., Urasawa, S., Taniguchi, K.: Sequential passages of human rotaviruses in MA 104 cells. Microbiol. Immunol.25, 1025–1035 (1981).

    Google Scholar 

  13. Woode, G. N., Bridger, J. C., Jones, J. M., Flewett, T. H., Bryden, A. S., Davies, H. A., White, G. B. B.: Morphological and antigenic relationships between rotaviruses from acute gastroenteritis of children, calves, piglets, mice and foals. Infect. Immun.14, 804–810 (1976).

    Google Scholar 

  14. Wyatt, R. G., Greenberg, H. B., James, W. D., Pittman, A. L., Kalica, A. R., Flores, J., Chanock, R. M., Kapikian, A. Z.: Definition of human rotavirus serotypes by plaque reduction assay. Infect. Immun.37, 110–115 (1982).

    Google Scholar 

  15. Wyatt, R. G., James, W. D., Pittman, A. L., Yasutaka, H., Greenberg, H. B., Kalica, A. R., Flores, J., Kapikian, A. Z.: Direct isolation in cell culture of human rotaviruses and their characterisation into four serotypes. J. Clin. Microbiol.18, 310–317 (1983).

    Google Scholar 

  16. Zissis, G., Lambert, J. P.: Different serotypes of human rotaviruses. Lanceti, 39 (1978).

    Google Scholar 

  17. Zissis, G., Lambert, J. P.: Enzyme-linked immunosorbent assays for serotyping of human rotavirus strains. J. Clin. Microbiol.11, 1–5 (1982).

    Google Scholar 

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Authors and Affiliations

  1. Regional Virus Laboratory and W.H.O. Collaborating Centre for Reference and Research on Rotaviruses, East Birmingham Hospital, Birmingham, United Kingdom

    G. M. Beards & T. H. Flewett

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  1. G. M. Beards
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  2. T. H. Flewett
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Beards, G.M., Flewett, T.H. Serological characterisation of human rotaviruses propagated in cell cultures. Archives of Virology 80, 231–237 (1984). https://doi.org/10.1007/BF01310663

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  • DOI: https://doi.org/10.1007/BF01310663

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