Summary
Electron microscopy and prolonged incubation of cell cultures have revealed the presence of a papovavirus in two foetal rhesus monkey kidney cell lines, FRhK-4 and FRhK-6, which are used to grow hepatitis A virus. The papovavirus, designated FRKV, was present in culture fluids from both cell lines and in thin sections of FRhK-4 cells. The size of the virus, 47 nm, places FRKV within the Polyomavirus genus. FRKV has been grown in primary human embryo kidney and calf kidney cell cultures. Haemagglutinin has not been demonstrated.
Preliminary investigations indicate that FRKV is antigenically identical to or closely related to stump-tailed macaque virus and is not one of the primate polyomaviruses SV40, SA12, BK or JC. FRKV reacts with antibody that occurs in pools of foetal and newborn bovine sera suggesting that the virus might be of bovine origin.
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Albert, A. E., ZuRhein, G. M.: Application of immune electron microscopy to the study of the antigenic relationships between three new human papovaviruses. Int. Arch. Allergy appl. Immunol.46, 405–416 (1974).
Almeida, J. D., Waterson, A. P.: The morphology of virus-antibody interaction. Adv. Virus Res.15, 307–338 (1969).
Bernhard, W., Vasquez, C., Tournier, P.: La structure du virus SV 40 étudiée par coloration négative en microscopie électronique. J. Micr., Paris1, 343–350 (1962).
Coackley, W., Maker, D., Smith, V. W.: A possible bovine polyomavirus. Arch. Virol.66, 161–166 (1980).
Crawford, L. V., Crawford, E. M.: A comparative study of polyoma and papilloma viruses. Virology21, 258–263 (1963).
Field, A. M., Gardner, S. D., Goodbody, R. A., Woodhouse, M. A.: Identity of a newly isolated human polyomavirus from a patient with progressive multifocal leucoencephalopathy. J. clin. Path.27, 341–347 (1974).
Flehmig, B.: Hepatitis A-virus in cell culture. I. Propagation of different hepatitis A-virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4). Med. Microbiol. Immunol.168, 239–248 (1980).
Gardner, S. D., Field, A. M., Coleman, D. V., Hulme, B.: New human papovavirus (B. K.) isolated from urine after renal transplantation. Lanceti, 1253–1257 (1971).
Granboulan, N., Tournier, P., Wicker, R., Bernhard, W.: An electron microscope study of the development of SV40 virus. J. Cell Biol.17, 423–441 (1963).
Hilleman, M. R., Provost, P. J., Buynak, E. B., MacLean, A. A.: Progress toward a hepatitis A vaccine. In: Standardization in Immunoprophylaxis of Hepatitis Viruses Infections (Second IABS Symposium on Viral Hepatitis, Athens 1982), Abstr. No. 77. World Health Organization 1982.
Howley, P. M., Newell, N., Shah, K. V., Law, M.-F., Gruss, P., Sauer, G., Kelly, T. J.: Identification of the primate papovavirus HD as the stump-tailed macaque virus. J. Virol.30, 400–403 (1979).
Lecatsas, G., Malherbe, H. H., Strickland-Cholmley, M.: Electronmicroscopical characterisation of simian papovavirus SA 12. J. med. Microbiol.10, 477–478 (1977).
Lecatsas, G., Prozesky, O. W., Scheepers, F.: The cytopathology and development of a human polyoma virus (B. K.). Arch. ges. Virusforsch.44, 319–327 (1974).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409–414 (1961).
Malherbe, H., Harwin, R., Ulrich, M.: The cytopathic effects of vervet monkey viruses. S. African med. J.37, 407–411 (1963).
Mattern, C. F. T., Takemoto, K. K., de Leva, A. M.: Electron microscopic observations on multiple polyoma virus-related particles. Virology32, 378–392 (1967).
Newell, N., Shah, K. V., Kelly, T. J.: Evolutionary relationships of the primate papovaviruses: base sequence homology among the genomes of simian virus 40, stump-tailed macaque virus, and SA12 virus. J. Virol.30, 624–636 (1979).
Newman, J. T., Smith, K. O.: Characteristics of a swine papovavirus. Infect. Immun.5, 961–967 (1972).
Parry, J. V.:In vitro propagation of hepatitis A virus and application of product to laboratory diagnosis. In: Proc. of the Second Internat. Hepatitis Workshop, Stirling, 1982, 15–23. Edinburgh: Nuclear Enterprises 1983.
Parry, J. V., Lucas, M. H., Richmond, J. E., Gardner, S. D.: Evidence for a bovine origin of the polyomavirus detected in foetal rhesus monkey kidney cells, FRhK-4 and -6. Arch. Virol.78, 151–165 (1983).
Penney, J. B., Weiner, L. P., Herndon, R. M., Narayan, O., Johnson, R. T.: Virions from progressive multifocal leukoencephalopathy: rapid serological identification by electron microscopy. Science178, 60–62 (1972).
Provost, P. J., Conti, P. A., Giesa, P. A., Banker, F. S., Buynak, E. B., McAleer, W. J., Hilleman, M. R.: Studies in chimpanzees of live, attenuated hepatitis A vaccine candidates. Proc. Soc. exp. Biol. Med.172, 357–363 (1983).
Provost, P. J., Hilleman, M. R.: Propagation of human hepatitis A virus in cell culturein vitro. Proc. Soc. exp. Biol. Med.160, 213–221 (1979).
Rangan, S. R. S., Lowrie, R. C., Roberts, J. A., Johnston, P. B., Warrick, R. P.: Virus from stumptailed monkey(Macaca arctoides) kidney cultures. Lab. Anim. Sci.24, 211–217 (1974).
Reissig, M., Kelly, T. J., Daniel, R. W., Rangan, S. R. S., Shah, K. V.: Identification of the stumptailed macaque virus as a new papovavirus. Infect. Immun.14, 225–231 (1976).
Shah, K., Nathanson, N.: Human exposure to SV40: review and comment. Amer. J. Epidem.103, 1–12 (1976).
Shah, K. V., Rangan, S. R. S., Reissig, M., Daniel, R. W., Beluhan, F. Z.: Congenital transmission of a papovavirus of the stump-tailed macaque. Science195, 404–406 (1977).
Sweet, B. H., Hilleman, M. R.: The vacuolating virus, S.V. 40. Proc. Soc. exp. Biol. Med.105, 420–427 (1960).
Tischer, I., Rasch, R., Tochtermann, G.: Characterization of papovavirus- and picornavirus-like particles in permanent pig kidney cell lines. Zbl. Bakt. Hyg. I Abt. Orig. A.226, 153–167 (1974).
Tumilowicz, J. J., Hung, C.-L., Kramarsky, B.: Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro15, 922–928 (1979).
Turnbull, P. C. B., Richmond, J. E.: A model of salmonella enteritis: the behaviour ofSalmonella enteritidis in chick intestine studied by light and electron microscopy. Br. J. exp. Path.59, 64–75 (1978).
Waldeck, W., Sauer, G.: New oncogenic papova virus from primate cells. Nature, Lond.269, 171–173 (1977).
Wallace, R. E., Vasington, P. J., Petricciani, J. C., Hopps, H. E., Lorenz, D. E., Kadanka, Z.: Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production. In Vitro8, 323–332 (1973).
Wallace, R. E., Vasington, P. J., Petricciani, J. C., Hopps, H. E., Lorenz, D. E., Kadanka, Z.: Development and characterization of cell lines from sub-human primates. In Vitro8, 333–341 (1973).
Wildy, P., Stoker, M. G. P., MacPherson, I. A., Horne, R. W.: The fine structure of polyoma virus. Virology11, 444–457 (1960).
Wrigley, N. G.: The lattice spacing of crystalline catalase as an internal standard of length in electron microscopy. J. Ultrastruct. Res.24, 454–464 (1968).
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Richmond, J.E., Parry, J.V. & Gardner, S.D. Characterisation of a Polyomavirus in two foetal rhesus monkey kidney cell lines used for the growth of hepatitis A virus. Archives of Virology 80, 131–146 (1984). https://doi.org/10.1007/BF01310654
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DOI: https://doi.org/10.1007/BF01310654