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In situ localization of ATPase activity in cells of plants infected by maize dwarf mosaic potyvirus

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Summary

Cells of healthy maize plants as well as those infected by maize dwarf mosaic potyvirus were examined by electron microscopy for the location of ATPase activity. In healthy and virus infected plants, ATPase activity was found in plasma membranes, chloroplast thylakoid membranes, nuclear membranes and in mitochondria. In virus-infected cells, ATPase activity was also observed in cytoplasmic vesicles which were found in close proximity to the virus-specific cytoplasmic inclusion bodies (CI), at the ends of the arms of the CI and in plasmodesmata.

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References

  1. Calder VL, Ingerfield M (1990) The roles of cylindrical inclusion protein of a potyvirus in the induction of vesicles and in cell-cell spread. J Struct Biol 105: 62–66

    Google Scholar 

  2. Chaffey NJ, Harris N (1985) Localization of ATPase activity on the plasmolemma of scutellar epithelial cells of germinating barley. J Exp Bot 36: 1612–1619

    Google Scholar 

  3. Eggen R, Van Kaman A (1988) RNA replication in comoviruses. In: Domongo E, Holland JJ, Ahlquist P (eds) RNA genetics, vol 1. CRC Press, Boca Raton, pp 49–69

    Google Scholar 

  4. Ford RE, Tosic M, Shukla DD (1989). Maize dwarf mosaic virus. AAB Descriptions of plant viruses, no. 34.

  5. Francki RIB, Milne RG, Hatta T (1985) Potyvirus group. In: Francki RIB, Milne RG, Hatta T (eds) Atlas of plant viruses, vol 2. CRC Press, Boca Raton, pp 183–217

    Google Scholar 

  6. Gianinazzi-Pearson V, Gianinazzi S (1982) Enzymatic studies on the metabolism of vesicular arbuscular mycorrhizas. IV. Ultra enzymological evidence (ATPase) for actual transfer processes in the host arbuscule interface. New Phytol 90: 37–43

    Google Scholar 

  7. Gorbalenya AE, Blinov VM, Donchenko AP, Koonin EV (1988). An NTP binding motif is the most conserved sequence in a highly divergent monophyletic group of proteins involved in positive strand RNA viral replication. J Mol Evol 28: 256–268

    Google Scholar 

  8. Habili N, Symons RH (1989) Evolutionary relationships between luteoviruses and other RNA plant viruses based on sequence motifs in their putative RNA polymerases and nucleic acid helicases. Nucleic Acids Res 17: 9543–9555

    Google Scholar 

  9. Hatta T, Francki RIB (1978) Enzyme cytochemical identification of single stranded and double stranded RNAs in virus-infected plant and insect cells. Virology 88: 105–117

    Google Scholar 

  10. Hoefert LL (1969) Proteinaceous and virus-like inclusions in cells infected with beet mosaic virus. Virology 37: 297–308

    Google Scholar 

  11. Joshi PA, Stewart JM, Graham ET (1988) Ultrastructural localization of ATPase activity in cotton fiber during elongation. Protoplasma 143: 1–10

    Google Scholar 

  12. Kim KS, Fulton JP (1969) Electron microscopy of pokeweed leaf cells infected with pokeweed mosaic virus. Virology 37: 297–308

    Google Scholar 

  13. Kras CJ, Ford RE (1969) Ultrastructure of corn systemically infected with maize dwarf mosaic virus. Phytopathology 59: 431–439

    Google Scholar 

  14. Lain S, Reichmann JL, Garcia JA (1990). RNA helicase a novel activity associated with a protein encoded by a positive strand RNA virus. Nucleic Acids Res 18: 7003–7006

    Google Scholar 

  15. Lain S, Martin MT, Reichmann JL, Garcia JA (1991). Novel catalytic activity associated with positive strand RNA virus infection: nucleic acid stimulated ATPase activity of the plum pox potyvirus helicase like protein. J Virol 65: 1–6

    Google Scholar 

  16. Langenberg WG (1986) Virus protein association with cylindrical inclusions of two viruses that infect wheat. J Gen Virol 67: 1161–1168

    Google Scholar 

  17. Lawson RH, Hearon SS (1971) The association of pinwheel inclusions with plasmodesmata. Virology 44: 454–456

    Google Scholar 

  18. Martelli GP, Russo M (1976) Unusual cytoplasmic inclusions induced by watermelon mosaic virus. Virology 72: 352–362

    Google Scholar 

  19. Strohmann H, Bickell-Sandkotter S (1984) Structure, function and regulation of chloroplast ATPase. Annu Rev Plant Physiol 35: 97–120

    Google Scholar 

  20. Troxler M, Egger D, Pfister T, Bienz K (1992) Intracellular localization of poliovirus RNA by in situ hybridization at the ultrastructural level using single stranded riboprobes. Virology 191: 687–697

    Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences, Wayne State University, Detroit, Michigan, USA

    S. Chen, P. Das & V. Hari

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  1. S. Chen
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  2. P. Das
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  3. V. Hari
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Chen, S., Das, P. & Hari, V. In situ localization of ATPase activity in cells of plants infected by maize dwarf mosaic potyvirus. Archives of Virology 134, 433–439 (1994). https://doi.org/10.1007/BF01310581

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  • DOI: https://doi.org/10.1007/BF01310581

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