Summary
Four monoclonal antibodies generated against the recombinant core antigen of hepatitis B virus are investigated for antigen binding. All exhibit a similar affinity to polystyrene-sorbed antigen but only one of them interacts with native form of HBcAg (an assembled particle) in solution. The presence of 0.1% sodium dodecylsulphate is required for the binding of other three antibodies. The phenomenon can be interpreted as inaccessibility of the corresponding epitopes unless the multimeric antigen structure is disrupted. The core antigen coated on polystyrene is considered as a similar exposed structure.
References
Bichko V, Pushko P, Dreilina D, Pumpen P, Gren E (1985) Subtype ayw variant of HBV DNA. Primary structure analysis. FEBS Lett 185: 208–212
Bichko V, Schödel F, Nassal M, Grene E, Berzinsh I, Borisova G, Miska S, Peterson DL, Gren E, Pushko P, Will H (1993) Epitopes recognized by antibodies to denatured core protein of HBV. Mol Immunol 30: 221–231
Birnbaum F, Nassal M (1990) HBV nucleocapsid assembly: primary structure requirements in the core protein. J Virol 64: 3319–3330
Borisova GP, Pumpen PP, Bichko VV, Pushko PM, Kalis JV, Dishler AV, Gren EJ (1984) Structure and expression of gene coding core antigen of human HBV inE. coli.. Dokl Akad Nauk SSSR (in Russian) 279: 1245–1248
Borisova G, Bundule M, Grinstein E, Dreilina D, Dreimane A, Kalis J, Kozlovskaya T, Loseva V, Ose V, Pumpen P, Pushko P, Snikere D, Stankevica E, Tsibinogin V, Gren E (1987) Recombinant capsid structures for exposure of protein antigenic epitopes. Mol Gen Life Sci Adv 6: 169–174
Borisova GP, Berzins I, Pushko PM, Pumpen P, Gren EJ, Tsibinogin VV, Loseva V, Ose V, Ulrich R, Siakkou H, Rosenthal HA (1989) Recombinant core particles of HBV exposing foreign antigenic determinants on their surface. FEBS Lett 259: 121–124
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72: 248–254
Clarke BE, Newton SE, Carroll AR, Francis MJ, Appleyard G, Syred A, Highfield PE, Rowlands DJ, Brown F (1987) Peptide vaccines based on enhanced immunogenicity of epitopes presented with T-cell determinants on hepatitis B core protein. Nature 330: 381–384
Dekker EL, Porta C, Van Regenmortel MHV (1989) Limitations of different ELISA procedures for localizing epitopes in viral coat protein subunits. Arch Virol 105: 269–286
Deutsch VR, Biadsi S, Eldor A, Muhlrad A, Kahane I (1990) Characterization of monoclonal antibodies to human platelet myosin that recognize highly conserved epitopes within the 50 kDa fragment. Biochim Biophys Acta 1038: 286–290
Ferns RB, Tedder RS (1984) Monoclonal antibodies to HBeAg derived from HBcAg-their use in characterization and detection of HBeAg. J Gen Virol 65: 899–908
Francis MJ, Hastings GZ, Brown AL, Grace KG, Rowlands DJ, Brown F, Clarke BE (1990) Immunological properties of hepatitis B core antigen fusion proteins. Proc Natl Acad Sci USA 87: 2545–2549
Friguet B, Chaffote AF, Djavadi-Ohanoiance L, Goldberg ME (1985) Measurements of the true affinity constant in solution of antigen-antibody complexes by ELISA. J Immunol Methods 77: 305–319
Ganem D, Varmus HE (1987) The molecular biology of hepatitis B virus. Annu Rev Biochem 56: 651–693
Hoogenrad NJ, Wraight CJ (1986) The effect of pristane on ascites tumor formation and monoclonal antibody production. In: Langone JJ, Van Vunakis H (eds) Methods in enzymology, vol 121. Academic Press, New York, pp 375–381
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Machida A, Ohnuma H, Takai E, Tsuda F, Tanaka T, Naito M, Munekata E, Miyakawa Y, Mayumi M (1989) Antigenic sites on the C-terminal domain of the capsid protein of HBV distinct from hepatitis B core or e antigen. Mol Immunol 26: 413–421
Malm B (1987) A method suitable for the isolation of monoclonal antibodies from large volumes of serum-containing hybridoma cell culture supernatants. J Immunol Methods 104: 103–109
Ohori H, Shimizu N, Yamada E, Onodera S, Ishida N (1984) Immunological and morphological properties of HBeAg subtypes 1 and 2 in HBV core particles. J Gen Virol 65: 405–414
Pasek M, Goto T, Gilbert W, Zink B, Schaller H, MacKay P, Leadbetter G, Murray K (1979) Hepatitis B virus genes and their expression inE. coli. Nature 282: 575–579
Salfeld J, Pfaff E, Noah M, Schaller H (1989) Antigenic determinants and functional domains in core antigen and e antigen from HBV. J Virol 63: 798–808
Sällberg M, Ruden U, Wahren B, Noah M, Magnius LO (1991) Human and murine B-cells recognize the HBeAg/beta (or HBe2) epitope as a linear determinant. Mol Immunol 28: 719–726
Skrivelis V, Steinberg Yu, Bichko V, Gren E, Tsimanis A (1993) The structure of the variable regions of mouse monoclonal antibodies to HBV core antigen. Scand J Immunol 37: 637–644
Stahl S, MacKay P, Magazin M, Bruce SA, Murray K (1982) HBV core antigen: synthesis inE. coli and application in diagnosis. Proc Natl Acad Sci USA 79: 1606–1610
Stahl S, Murray K (1989) Immunogenicity of peptide fusions to HBV core antigen. Proc Natl Acad Sci USA 86: 6283–6287
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Apsalons, U., Bichko, V. The affinities of monoclonal antibodies against core antigen of hepatitis B virus. Archives of Virology 134, 393–402 (1994). https://doi.org/10.1007/BF01310576
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DOI: https://doi.org/10.1007/BF01310576