Skip to main content
SpringerLink
Log in

Molecular epidemiology of human rotaviruses in Manipur: genome analysis of rotaviruses of long electropherotype and subgroup I

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

In 1987/88 a winter outbreak of infantile gastroenteritis occurred in Manipur, India which was mainly due to rotaviruses of long electropherotype and subgroup (SG) I. The VP7 gene of one of these viruses (M48) has been cloned and sequenced. It was found to be very closely related to the VP7 genes of the G2 serotype human rotaviruses RV-5 and S2. Follow-up epidemiology of this event in Manipur during 1989–1992 yielded mainly rotaviruses of more conventional characteristics (94 isolates of SG II and long electropherotype, and 90 isolates of SG I and short electropherotype), but also 6 isolates of SG I with long electropherotype, indicating that these viruses continue to circulate in the Manipur community. One isolate of short electropherotype was of subgroup II, and one long electropherotype isolate reacted with the group A but not with either the subgroup I or subgroup II monoclonal antibodies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aboudy J, Shif I, Zilberstein I, Gotlieb-Stematsky T (1988) Use of polyclonal and monoclonal antibodies and analysis of viral RNA in the detection of unusual group A human rotaviruses. J Med Virol 25: 351–359

    Google Scholar 

  2. Ahmed MU, Urasawa S, Taniguchi K, Urasawa T, Kobayashi N, Wakasugi F, Islam AIMM, Sahikh HA (1991) Analysis of human rotavirus strains prevailing in Bangladesh in relation to nationwide floods brought by the 1988 monsoon. J Clin Microbiol 29: 2273–2279

    Google Scholar 

  3. Beards G, Desselberger U, Flewett TH (1989) Temporal and geographical distributions of human rotavirus serotypes, 1983–1988. J Clin Microbiol 27: 2827–2833

    Google Scholar 

  4. Beards G, Xu L, Ballard A, Desselberger U, McCrae MA (1992) A serotype 10 human rotavirus. J Clin Microbiol 30: 1432–1435

    Google Scholar 

  5. Bellinzoni RB, Mattion NM, Matson DO, Blackhall J, La Torre JL, Scodeller EA, Urasawa S, Taniguchi K, Estes MK (1990) Porcine rotaviruses antigenically related to human rotavirus serotypes 1 and 2. J Clin Microbiol 28: 633–636

    Google Scholar 

  6. Blackhall J, Bellinzoni R, Mattion N, Estes MK, La Torre JL, Magnusson G (1992) A bovine rotavirus serotype 1: serologic characterization of the virus and nucleotide sequence determination of the structural glycoprotein VP7 gene. Virology 189: 833–837

    Google Scholar 

  7. Both GW, Mattick JS, Bellamy AR (1983) Serotype specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence. Proc Natl Acad Sci USA 80: 3091–3095

    Google Scholar 

  8. Browning GF, Chalmers RM, Fitzgerald TA, Snodgrass DR (1991a) Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype. J Gen Virol 72: 1059–1064

    Google Scholar 

  9. Browning GF, Fitzgerald TA, Chalmers RM, Snodgrass DR (1991b) A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI23. J Clin Microbiol 29: 2043–2046

    Google Scholar 

  10. Das M, Dunn SJ, Woode GN, Greenberg HB, Rao CD (1993) Both surface proteins (VP4 and VP7) of an asymptomatic neonatal rotavirus strain (1321) have high levels of sequence identity with the homologous proteins of a serotype 10 bovine rotavirus. Virology 194: 374–379

    Google Scholar 

  11. Desselberger U (1993) Towards rotavirus vaccines. Rev Med Virol 3: 15–21

    Google Scholar 

  12. Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the Vax. Nucleic Acids Res 12: 387–395

    Google Scholar 

  13. Dunn SJ, Greenberg HB, Ward RL, Nakagomi O, Burns JW, Vo PT, Pax KA, Das M, Gowda K, Rao CD (1993) Serotypic and genotypic characterization of human serotype 10 rotaviruses from asymptomatic neonates. J Clin Microbiol 31: 165–169

    Google Scholar 

  14. Dyall-Smith ML, Holmes IH (1984) Sequence homology between human and animal rotavirus serotype-specific glycoproteins. Nucleic Acids Res 12: 3973–3982

    Google Scholar 

  15. Estes MK, Cohen J (1989) Rotavirus gene structure and function. Microbiol Rev 53: 410–449

    Google Scholar 

  16. Follett EAC, Sanders RC, Beards GM, Hundley F, Desselberger U (1984) Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the West of Scotland 1981/82 and 1982/83. J Hyg 92: 209–222

    Google Scholar 

  17. Garbarg-Chenon A, Bricout F, Nicolas JC (1986) Serological characterization of human reassortant rotaviruses. J Virol 59: 510–513

    Google Scholar 

  18. Gentsch JR, Das BK, Jiang B, Bhan MK, Glass RI (1993) Similarity of the VP4 protein of human rotavirus strain 116E to that of the bovine B 223 strain. Virology 194: 424–430

    Google Scholar 

  19. Gerna G, Sarasini A, Matteo AD, Zentilin L, Miranda P, Parea M, Baldanti F, Arista S, Milanesi G, Battaglia M (1990) Serotype 3 human rotavirus strains with subgroup I specificity. J Clin Microbiol 28: 1342–1347

    Google Scholar 

  20. Gerna G, Sarasini A, Parea M, Arista S, Miranda P, Brussow H, Hoshino Y, Flores J (1992) Isolation and characterization of two distinct human rotavirus strains with G6 specificity. J Clin Microbiol 30: 9–16

    Google Scholar 

  21. Ghosh SK, Naik TN (1989) Detection of a large number of subgroup I human rotaviruses with a “long” RNA electropherotype. Arch Virol 105: 119–127

    Google Scholar 

  22. Glass RI, Keith J, Nakagomi O, Nakagomi T, Askaa J, Kapikian AZ, Chanock RM, Flores J (1985) Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhoea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology 141: 292–298

    Google Scholar 

  23. Gorziglia M, Aguirre Y, Hoshino Y, Esparza J, Blumenthal I, Askaa J, Thompson M, Glass RI, Kapikian AZ, Chanock RM (1986) VP7 serotype-specific glycoprotein of OSU porcine rotavirus: coding assignment and gene sequence. J Gen Virol 67: 2445–2454

    Google Scholar 

  24. Gorziglia M, Nishikawa K, Green K, Taniguchi K (1988) Gene sequence of the VP7 serotype specific glycoprotein of Gottfried porcine rotavirus. Nucleic Acids Res 16: 775

    Google Scholar 

  25. Green KY, Hoshino Y, Ikegami N (1989) Sequence analysis of the gene encoding the serotype-specific glycoprotein (VP7) of two new human rotavirus serotypes. Virology 168: 429–433

    Google Scholar 

  26. Green KY, Midthun K, Gorziglia M, Hoshino Y, Kapikian AZ, Chanock RM, Flores J (1987) Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology 161: 153–159

    Google Scholar 

  27. Gunn PR, Sato F, Powell KFH, Bellamy AR, Napier JR, Harding DRK, Hancock WS, Siegman LJ, Both GW (1985) Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis. J Virol 54: 791–797

    Google Scholar 

  28. Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD (1982) Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver stained polyacrylamide gels. J Clin Microbiol 16: 473–477

    Google Scholar 

  29. Hum CP, Dyall-Smith ML, Holmes IH (1989) The VP7 gene of a new G serotype of human rotavirus (B37) is similar to G3 proteins in the antigenic C region. Virology 170: 55–61

    Google Scholar 

  30. Kantharidis P, Dyall-Smith ML, Holmes IH (1987) Marked sequence variation between segment 4 genes of human RV-5 and simian SA11 rotaviruses. Arch Virol 93: 111–121

    Google Scholar 

  31. Kapikian AZ, Chanock RM (1990) Rotaviruses. In: Fields BN, Knife DM, Chanock RM, Hirsch MS, Melmick JL, Monath TP, Roizman B, (eds) Virology, 2nd edn. Raven Press, New York, pp 1353–1404

    Google Scholar 

  32. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685

    Google Scholar 

  33. Matsuda Y, Nakagomi O (1989) Antigenic and molecular characterization of bovine rotaviruses isolated in Japan. Res Virol 140: 337–350

    Google Scholar 

  34. Nakagomi T, Katsushima N, Nakagomi O (1988) Relative frequency of human rotavirus subgroups I and II in relation to ‘short’ and ‘long’ electropherotypes of viral RNA. Ann Inst Pasteur 139: 295–300

    Google Scholar 

  35. Nakagomi T, Nakagomi O (1989) RNA-RNA hybridization identifies a human rotavirus that is genetically related to feline rotavirus. J Virol 63: 1431–1434

    Google Scholar 

  36. Nakagomi O, Ohshina A, Aboudy Y, Shif I, Mochizuki M, Nakagomi T, Gotlieb-Stematsky T (1990) Molecular identification by RNA-RNA hybridization of a human rotavirus that is closely related to rotaviruses of feline and canine origin. J Clin Microbiol 28: 1198–1203

    Google Scholar 

  37. Nishikawa K, Hoshino Y, Gorziglia M (1991) Sequence of the VP7 gene of chicken rotavirus Ch2 strain of serotype 7 rotavirus. Virology 185: 853–857

    Google Scholar 

  38. Noel JS, Beards GM, Cubitt WD (1991) Epidemiological survey of human rotavirus serotypes and electropherotypes in young children admitted to two children's hospitals in northeast London from 1984–1990. J Clin Microbiol 29: 2213–2219

    Google Scholar 

  39. Richardson MA, Iwamoto A, Ikegami N, Nomoto A, Furuichi Y (1984) Nucleotide sequence of the gene encoding the serotype-sepecific antigen of human (Wa) rotavirus: Comparison with the homologous genes from simian SA11 and UK bovine rotaviruses. J Virol 51: 860–862

    Google Scholar 

  40. Rodger SM, Holmes IH (1979) Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. J Virol 30: 839–846

    Google Scholar 

  41. Ruiz AM, Lopez IV, Lopez S, Espejo RT, Arias CF (1988) Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype. J Virol 62: 4331–4336

    Google Scholar 

  42. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning — a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Springer Harbor

    Google Scholar 

  43. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    Google Scholar 

  44. Staden R (1984) A computer program to enter DNA gel reading data into a computer. Nucleic Acids Res 12: 499–503

    Google Scholar 

  45. Steele D, Alexander JJ (1988). The relative frequency of subgroup I and II rotaviruses in black infants in Sourth Africa. J Med Virol 24: 321–327

    Google Scholar 

  46. Steele AD, van Niekerk MC, Geyer A, Bos P, Alexander JJ (1992). Further characterization of human rotaviruses isolated from asymptomatically infected neonates in South Africa. J Med Virol 38: 22–26

    Google Scholar 

  47. Sukumaran M, Gowda K, Maiya PP, Srinivas TP, Kumar MS, Aijaz S, Reddy RR, Padilla L, Greenberg HB, Rao CD (1992) Exclusive asymptomatic neonatal infections by human rotavirus strains having subgroup I specificity and “long” RNA electropherotype. Arch Virol 126: 239–251

    Google Scholar 

  48. Tabor S, Richardson CC (1989) Selective inactivation of the exonuclease activity of bacteriophage T7 DNA polymerase by in vitro mutagenesis. J Biol Chem 264: 6447–6458

    Google Scholar 

  49. Taniguchi K, Urasawa T, Kobayashi N, Gorziglia M, Urasawa S (1990) Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity. J Virol 64: 5640–5644

    Google Scholar 

  50. Taniguchi K, Urasawa T, Pongsuwanna J, Choonthanom M, Tayavasu C, Urasawa S (1991) Molecular and antigenic analyses of serotypes 8 and 10 of bovine rotaviruses in Thailand. J Gen Virol 72: 2929–2937

    Google Scholar 

  51. Urasawa S, Hasegawa A, Urasawa T, Taniguchi K, Wakasugi F, Suzuki H, Inouye S, Pongprot B, Supawadee J, Suprasert S, Rangsiyanond P, Tonusin S, Yamazi Y (1992) Antigenic and genetic analyses of human rotaviruses in Chiang Mai, Thailand: Evidence for a close relationship between human and animal rotaviruses. J Infect Dis 166: 227–234

    Google Scholar 

  52. Xu L, Harbour D, McCrae MA (1990) the application of polymerase chain reaction to the detection of rotaviruses in faeces. J Virol Methods 27: 29–38

    Google Scholar 

  53. Xu L, Harbour D, McCrae MA (1991) Sequence of the gene encoding the major neutralization antigen (VP7) of serotype 10 rotavirus. J Gen Virol 72: 177–180

    Google Scholar 

Download references

Author information

Author notes

  1. S. Shen

    Present address: Lanzhou Institute of Biological Products, Lanzhou, Gansu, China

Authors and Affiliations

  1. Clinical Microbiology and Public Health Laboratory, and Division of Virology, Department of Pathology, University of Cambridge, Cambridge, UK

    T. Krishnan, B. Burke, S. Shen & U. Desselberger

  2. National Institute of Cholera and Enteric Diseases, Calcutta, India

    T. Krishnan & T. N. Naik

Authors
  1. T. Krishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Burke
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. N. Naik
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. U. Desselberger
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krishnan, T., Burke, B., Shen, S. et al. Molecular epidemiology of human rotaviruses in Manipur: genome analysis of rotaviruses of long electropherotype and subgroup I. Archives of Virology 134, 279–292 (1994). https://doi.org/10.1007/BF01310567

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01310567

Keywords

Search

Navigation