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HSV infected RAJI-cells specify HSV specific immediate early and/or early DNA-binding proteins

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Summary

Herpes Simplex Virus specified DNA-binding proteins were purified from virus infected VERO and RAJI cells. The expression of the proteins differed depending on the type of the host cell. Polypeptides corresponding to HSV-1 specific ICP 8 and HSV-2 specific ICSP 11/12 were the major products of HSV-infected VERO cells. In RAJI cells polypeptides with a corresponding molecular weight, together with a cellular protein having almost similar mobility on SDS gels could be detected. Using immunoblotting technique the HSV origin of these 135K molecular weight proteins synthesized in the HSV-1 and HSV-2-infected RAJI cells could be confirmed.

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References

  1. Aurelian, L., Manak, M. M., McKinlay, M., Smith, C. C., Klacsmann, K. T., Gupta, P. K.: The herpesvirus hypothesis — are Koch's postulates satisfied? Gynecol. Oncology12, 56–87 (1981).

    Google Scholar 

  2. Arsenakis, M., Roizman, B.: A post-α gene function turns off the capacity of a host protein to bind DNA in cells infected with herpes simplex virus 1. J. Virol.49, 813–818 (1984).

    Google Scholar 

  3. Bonner, W. M., Laskey, R. A.: A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem.46, 83–88 (1974).

    Google Scholar 

  4. Dreesman, G. R., Burek, J., Adam, E., Kaufman, R. H., Melnick, J. L., Powell, K. L., Purifoy, D. J. M.: Expression of herpesvirus induced antigens in human cervical cancer. Nature282, 591–593 (1980).

    Google Scholar 

  5. Epstein, A. L., Jacquemont, B.: Virus polypeptide synthesis induced by herpes simplex virus in non-permissive rat XC cells. J. gen. Virol.64, 1499–1508 (1983).

    Google Scholar 

  6. Freeman, M. J., Powell, K. L.: DNA-binding properties of a herpes simplex virus immediate early protein. J. Virol.44, 1084–1087 (1982).

    Google Scholar 

  7. Flannery, V. L., Courtney, R. J., Schaffer, P. A.: Expression of an early non-structural antigen of herpes simplex virus in cells transformed by herpes simplex virus. J. Virol.21, 284–301 (1977).

    Google Scholar 

  8. Godowski, P. J., Knipe, D. M.: Mutations in the major DNA-binding protein gene of herpes simplex virus type 1 results in increased levels of viral gene expression. J. Virol.47, 478–486 (1983).

    Google Scholar 

  9. Galloway, D. A., Swain, M. A.: Organization of the left-hand end of the herpes simplex virus type 2 Bg/II N fragment. J. Virol.49, 724–730 (1984).

    Google Scholar 

  10. Giraldo, G., Beth, E., Hämmerling, U., Tarro, G., Kourilsky, F. M.: Detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anticomplement immunofluorescence and use of a blocking assay to demonstrate their specificity. Int. J. Cancer19, 107–116 (1977).

    Google Scholar 

  11. Hammer, S. M., Richter, B. S., Hirsch, M. S.: Activation and suppression of herpes simplex virus in a human T lymphoid cell line. J. Immunol.127, 144–148 (1981).

    Google Scholar 

  12. Kallin, B., Klein, G.: Epstein-Barr virus carried by Raji cells a mutant in early functions? Intervirology19, 47–51 (1983).

    Google Scholar 

  13. Kessler, I. I.: Human cervical cancer as a venereal disease. Cancer Res.36, 783–791 (1976).

    Google Scholar 

  14. Knipe, D. M., Quinlan, M. P., Spang, A. E.: Characterization of two conformational forms of the major DNA-binding protein enclosed by Herpes Simplex virus 1. J. Virology44, 736–741 (1982).

    Google Scholar 

  15. Lehtinen, M., Lehtinen, T., Koivisto, V., Paavonen, J., Leinikki, P.: Serum antibodies to the major HSV-2 specified DNA-binding protein in patients with an acute HSV infection or cervical neoplasia. J. Med. Virol.16, 245–256 (1985a).

    Google Scholar 

  16. Lehtinen, M., Rantala, I., Teisala, K., Heinonen, P. K., Lehtinen, T., Aine, R., Miettinen, A., Grönroos, P., Punnonen, R., Leinikki, P., Paavonen, J.: Detection of herpes simplex virus in patients with acute salpingitis. J. Inf. Dis.152, 145–152 (1985b).

    Google Scholar 

  17. Leinbach, S. S., Summers, W. C.: Herpes simplex virus type 1 infection of isogenic Epstein-Barr virus genome-negative and positive Burkits's lymphoma derived cell lines. J. Virol.30, 248–254 (1979).

    Google Scholar 

  18. Leinikki, P.: Immunofluorescent assay of herpesvirus type 1 and 2 antibodies in rabbit and human sera. Arch. ges. Virusforsch.35, 349–355 (1971).

    Google Scholar 

  19. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.192, 265–275 (1951).

    Google Scholar 

  20. McDougall, J. K., Crum, C. P., Fenoglio, C. M., Goldstein, L. C., Galloway, D. A.: Herpesvirus-specific RNA and protein in carcinoma of the uterine cervix. Proc. Natl. Acad. Sci. U.S.A.79, 3853–3857 (1982).

    Google Scholar 

  21. Melnick, J. L., Adam, E., Lewis, R., Kaufman, R. H.: Cervical cancer cell lines containing herpesvirus markers. Intervirology12, 111–114 (1979).

    Google Scholar 

  22. Nishiyama, Y., Rapp, F.: Regulation of persistent infection with herpes simplex virusin vitro by hydrocortisone. J. Virol.31, 841–844 (1979).

    Google Scholar 

  23. Powell, K. L., Littler, E., Purifoy, D. J. M.: Nonstructural proteins of herpes simplex virus 11. Major virus-specific DNA-binding protein. J. Virol.39, 894–901 (1981).

    Google Scholar 

  24. Preston, C. M.: Control of herpes simplex virus type 1 mRNA synthesis in cells infected with wild type virus or the temperature sensitive mutant tsK. J. Virol.29, 275–284 (1979).

    Google Scholar 

  25. Purifoy, D. J. M., Powell, K. L.: DNA-binding proteins induced by herpes simplex virus type 2 in Hep-2 cells. J. Virol.19, 717–731 (1976).

    Google Scholar 

  26. Rice, G. P. A., Schrier, R. D., Oldstone, M. B. A.: Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products. Proc. Natl. Acad. Sci. U.S.A.81, 6134–6138 (1984).

    Google Scholar 

  27. Rinaldo, C. R., Richter, B. S., Black, P. H., Hirsh, M. S.: Persistent infection of human lymphoid and myeloid cell lines with herpes simplex virus. Infect. Immun.25, 521–525 (1979).

    Google Scholar 

  28. Roubal, J., Kallin, B., Luka, J., Klein, G.: Early DNA-binding polypeptides of Epstein-Barr virus. Virology113, 285–292 (1981).

    Google Scholar 

  29. Seigneurin, J.-M., Desgranges, C., Lavoue, M.-F.,de-The, G.: Herpes simplex-Raji (A44), a new cell line for serologic testing by immunofluorescence. J. Immunol.117, 950–957 (1976).

    Google Scholar 

  30. Sugawara, K., Kawanishi, M., Ito, Y.: Epstein-Barr virus-related DNA-binding proteins induced by n-butyrate in P3HR-1 cells. Virology116, 354–358 (1982).

    Google Scholar 

  31. Teute, H., Braun, R., Kirchner, H., Becker, H., Munk, K.: Replication of herpes simplex virus in human T lymphocytes. Intervirology20, 32–41 (1983).

    Google Scholar 

  32. Yeo, J., Killington, R. A., Watson, D. H., Powell, K. L.: Studies on the cross-reacting antigens in the herpes viruses. Virology108, 256–266 (1981).

    Google Scholar 

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  1. Institute of Biomedical Sciences, University of Tampere, Tampere, Finland

    M. Lehtinen

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Lehtinen, M. HSV infected RAJI-cells specify HSV specific immediate early and/or early DNA-binding proteins. Archives of Virology 87, 107–118 (1986). https://doi.org/10.1007/BF01310547

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  • DOI: https://doi.org/10.1007/BF01310547

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