Summary
The presence of Hepatitis B virus (HBV) DNA in sera of patients with HBV related diseases is considered a reliable marker of virus active replication. In this paper HBV DNA was assayed by the molecular hybridization method with a32P labeled nick translated HBV probe. The assay was positive in sera of 21 out of 22 HBeAg-positive and 4 out of 8 HBeAg-negative asymptomatic HBsAg carriers. 15 HBsAg-negative sera obtained from healthy donors showed no HBV DNA. Almost 80 per cent of HBeAg and HBV DNA positive sera revealed a DNA-polymerase activity. In order to determine the infectivity of HBsAg carriers, it appears that, whenever possible, the HBV DNA spot hybridization should be performed in conjunction with the DNA-polymerase, HBsAg and HBeAg tests.
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References
Tiollais, P., Charnay, P., Vyas, G. N.: Biology of hepatitis B virus. Science213, 406–411 (1981).
Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol.98, 503–517 (1975).
Brechot, C., Pourcel, C., Louise, A., Rain, B., Tiollais, P.: Presence of integrated hepatitis B virus DNA in cellular DNA of human hepatocellular carcinoma. Nature286, 533–535 (1980).
Edman, J. C., Gray, P. G., Valenzuela, P., Rall, L. B., Rutter, W. J.: Integration of hepatitis B virus sequences and their expression in human hepatoma cell. Nature286, 535–537 (1980).
de Jean, A., Carloni, G., Brechot, C., Tiollais, P., Wain-Hobson, S.: Organization and expression of hepatitis B sequences cloned from hepatocellular carcinoma tissue DNA. J. Cell. Biochem.20, 293–301 (1982).
Brechot, C., Hadchouel, M., Scotto, J., Fonk, M., Potet, F., Vyas, G. N., Tiollais, P.: State of hepatitis B virus DNA in hepatocytes of patients with hepatitis B surface antigen-positive and -negative liver diseases. Proc. Natl. Acad. Sci. U.S.A.78, 3906–3910 (1981).
Brechot, C., Hadchouel, M., Scotto, J., Degos, F., Charnay, P., Trepo, C., Tiollais, P.: Detection of hepatitis B virus DNA in liver and serum: a direct appraisal of the chronic carrier state. Lancetii, 765–768 (1981).
Brechot, C., Pourcel, C., Hadchouel, M., de Jean, A., Louise, A., Scotto, J., Tiollais, P.: State of hepatitis B virus DNA in liver diseases. Hepatology2, 278–285 (1982).
Bonino, F., Hoyer, B., Nelson, J., Engle, R., Verme, G., Gerin, J. L.: Hepatitis B virus DNA in the sera of HBsAg carriers: a marker of active hepatitis B virus replication in the liver. Hepatology1, 386–391 (1981).
Berninger, M., Hemmer, M., Hoyer, B., Gerin, J. L.: An assay for the detection of the DNA genome of hepatitis B virus in serum. J. Med. Virol.9, 57–68 (1982).
Weller, I. V. D., Fowler, M. J. F., Monjardino, J., Thomas, H. C.: The detection of HBV DNA in serum by molecular hybridization: a more sensitive method for the detection of complete HBV particles. J. Med. Virol.9, 273–280 (1982).
Kam, W., Rall, L. B., Smuckler, E. A., Schmid, R., Rutter, W. J.: Hepatitis B viral DNA in liver and serum of asymptomatic carriers. Proc. Natl. Acad. Sci. U.S.A.79, 7522–7526 (1982).
Scotto, J., Hadchouel, M., Hery, C., Yvart, J., Tiollais, P., Brechot, C.: Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: comparison with results for other viral markers. Hepatology3, 279–284 (1983).
Denhardt, D. V.: Membrane filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Comm.23, 641–646 (1966).
Dubois, M. F., Pourcel, C., Rousset, S., Chany, C., Tiollais, P.: Excretion of hepatitis B virus surface antigen particles from mouse cells transformed with cloned viral DNA. Proc. Natl. Acad. Sci. U.S.A.77, 4549–4553 (1980).
Rigby, P. W. J., Dieckmann, M., Rhodes, C., Berg, P.: Labeling deoxyribonucleic acid to high specific activityin vitro by nick translation with DNA-polymerase I. J. Mol. Biol.113, 237–251 (1977).
Kaplan, P. M., Greenman, R. L., Gerin, J. L., Purcell, R. H., Robinson, W. S.: DNA-polymerase associated with human hepatitis B antigen. J. Virol.12, 995–1005 (1973).
Shafritz, D. A., Shouval, D., Sherman, H. I., Hadziyannis, S. J., Kew, M. C.: Integration of hepatitis B virus DNA into the genome of liver cells in chronic liver disease and hepatocellular carcinoma. N. Engl. J. Med.305, 1067–1073 (1981).
Werner, B. G., O'Connel, A. P., Summers, J.: Association of e antigen with Dane particles DNA in sera from asymptomatic carriers of hepatitis B surface antigen. Proc. Natl. Acad. Sci. U.S.A.74, 2149–2151 (1977).
Alter, H. J., Seef, L. B., Kaplan, P. M., McAuliffe, W. J., Wright, E. C., Gerin, J. L., Purcell, R. H., Holland, P. V., Zimmermann, H. J.: Type B hepatitis: the infectivity of blood positive for e antigen and DNA-polymerase after needlestick exposure. N. Engl. J. Med.295, 909–913 (1976).
Ricci, G., de Bac, C., Caramia, F.: Carriers of hepatitis B antigen: an epidemiologic and histologic study. J. Infect. Dis.128, 125–128 (1973).
Miller, R. H., Tran, C. T., Robinson, W. S.: Hepatitis B particles of plasma and liver contain viral DNA-RNA hybrids molecules. Virology139, 53–63 (1984).
Summers, J., Mason, W. S.: Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate. Cell29, 403–415 (1982).
Tur-Kaspa, R., Keshet, E., Eliakim, M., Shouval, D.: Detection and characterization of hepatitis B virus DNA in serum of HBeAg-negative HBsAg carriers. J. Med. Virol.14, 17–26 (1984).
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Carloni, G., Delfini, C., Colloca, S. et al. Incidence of Hepatitis B virus DNA and DNA-polymerase in sera of Italian asymptomatic carriers with the serological markers of HBV. Archives of Virology 87, 97–105 (1986). https://doi.org/10.1007/BF01310546
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DOI: https://doi.org/10.1007/BF01310546