Skip to main content
SpringerLink
Log in

Humoral and cell-mediated immune responses to the glycoproteins of infectious laryngotracheitis herpesvirus

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

The viral glycoproteins of infectious laryngotracheitis virus, an alphaherpesvirus, were the dominant antigens recognised by immune chickens. Glycoproteins with molecular weights of 205, 160, 115, 90, 67, 60, and 52 k reacted strongly in Western blotting studies with a majority of chicken antisera. Viral glycoproteins immunoprecipitated using monoclonal antibodies were also able to elicit a delayed-type hypersensitivity reaction in chickens previously vaccinated with a live vaccine. The 60 k glycoprotein alone and the antigenically related family of higher molecular weight glycoproteins (205, 160, 115, 90, and 85 k) both elicited significant increased in the thickness of the wattles of immune cockerels. Because the glycoproteins induce both antibody and cell-mediated immune responses they may prove to be important protective immunogens in a subunit vaccine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Burnette WN (1981) ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112: 195–203

    Google Scholar 

  2. Chan WL (1983) Protective immunization of mice with specific HSV-1 glycoproteins. Immunology 49: 343–352

    Google Scholar 

  3. Cremer KJ, Mackett M, Wohlenberg C, Notkins AL, Moss B (1985) Vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein D prevents latent herpes in mice. Science 228: 737–739

    Google Scholar 

  4. Eberle R, Mou S-W (1983) Relative titers of antibodies to individual polypeptide antigens of herpes simplex virus type 1 in human sera. J Infect Dis 148: 436–444

    Google Scholar 

  5. Eberle R, Mou S-W, Zaia JA (1985) The immune response to herpes simplex virus: comparison of the specificity and relative titers of serum antibodies directed against viral polypeptides following primary herpes simplex virus type 1 infections. J Med Virol 16: 147–162

    Google Scholar 

  6. Fahey KJ, Bagust TJ, York JJ (1983) Laryngotracheitis infection in the chicken: the role of humoral antibody in immunity to a graded challenge infection. Avian Pathol 12: 505–514

    Google Scholar 

  7. Fahey KJ, York JJ, Bagust TJ (1984) Laryngotracheitis herpesvirus infection in the chicken II. The adoptive transfer of resistance with immune spleen cells. Avian Pathol 13: 265–275

    Google Scholar 

  8. Hanson LE (1984) Laryngotracheitis. In: Hofstad MS, Barnes HJ, Calnek BW, Reid WM, Yoder HW Jr, (eds) Diseases of poultry, 8 th edn. Iowa State University Press, Ames, Iowa, pp 444–451

    Google Scholar 

  9. Kahlon J, Lakeman FD, Ackermann M, Whitley RJ (1986) Human antibody response to herpes simplex virus-specific polypeptides after primary and recurrent infection. J Clin Microbiol 23: 725–730

    Google Scholar 

  10. Lasky LA, Dowbenko D, Simonsen CC, Berman PW (1984) Protection of mice from lethal herpes simplex virus infection by vaccination with a secreted form of cloned glycoprotein D. Bio/Technology 2: 527–532

    Google Scholar 

  11. Long D, Madara TJ, Ponce de Leon M, Cohen GH, Montgomery PC, Eisenberg RJ (1984) Glycoprotein D protects mice against lethal challenge with herpes simplex virus types 1 and 2. Infect Immun 37: 761–764

    Google Scholar 

  12. Marchioli CC, Yancey RJ Jr, Petrovskis EA, Timmins JG, Post LE (1987) Evaluation of pseudorabies virus glycoprotein gp 50 as a vaccine for Aujeszky's disease in mice and swine: expression by vaccinia virus and Chinese hamster ovary cells. J Virol 61: 3977–3982

    Google Scholar 

  13. Marsden HS (1987) Herpes simplex virus glycoproteins and pathogenesis. In: Russell WC, Almond JW (ed) Molecular basis of virus disease. 40 th symposium of the Society for General Microbiology. Cambridge University Press, Cambridge, pp 259–288

    Google Scholar 

  14. Paoletti E, Lipinskas BR, Samsonoff C, Mercer S, Panicali D (1984) Construction of live vaccines using genetically engineered poxviruses: biological activity of vaccinia virus recombinants expressing the hepatitis B virus surface antigen and the herpes simplex virus glycoprotein D. Proc Natl Acad Sci USA 81: 193–197

    Google Scholar 

  15. Robertson GM (1977) The role of bursa-dependent responses in immunity to infectious laryngotracheitis. Res Vet Sci 22: 281–284

    Google Scholar 

  16. Schrier RD, Pizer LI, Moorhead JW (1983) Type-specific delayed hypersensitivity and protective immunity by isolated herpes simplex virus glycoprotein. J Immunol 130: 1413–1418

    Google Scholar 

  17. Scott NA, Whalley JM, Mattick JS, Underwood PA, Aboud L, Williams KL, Kirkland P (1988) Identification of major antigenic proteins of bovine herpesvirus 1 and their correlation with virus neutralizing activity. Vet Microbiol 16: 109–121

    Google Scholar 

  18. Teglbjaerg CS, Feldborg R, Norrild B (1986) Immunological reactivity of human sera with individual herpes simplex proteins: a comparative study of sera from patients with preinvasive or invasive cervical cancer and from controls. J Med Virol 18: 169–180

    Google Scholar 

  19. Todd D, Hull J, McNair J (1987) Antigenically important proteins of Aujeszky's disease (pseudorabies) virus identified by immunoblotting. Arch Virol 96: 215–224

    Google Scholar 

  20. van Drunen Littel-van den Hurk S, Babiuk LA (1986) Polypeptide specificity of the antibody response after primary and recurrent infection with bovine herpesvirus 1. J Clin Microbiol 23: 274–282

    Google Scholar 

  21. Weir JP, Bennett M, Allen EM, Elkins KL, Martin S, Rouse BT (1989) Recombinant vaccinia virus expressing the herpes simplex virus type 1 glycoprotein C protects mice against herpes simplex virus challenge. J Gen Virol 70: 2587–2594

    Google Scholar 

  22. Wildly P, Gell PGH (1985) The host response to herpes simplex virus. Br Med Bull 41: 86–91

    Google Scholar 

  23. York JJ, Sonza S, Fahey KJ (1987) Immunogenic glycoproteins of infectious laryngotracheitis herpesvirus. Virology 161: 340–347

    Google Scholar 

  24. York JJ, Sonza S, Brandon MR, Fahey KJ (1990) Antigens of infectious laryngotracheitis herpesvirus defined by monoclonal antibodies. Arch Virol 115: 147–162

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia

    Jennifer J. York & K. J. Fahey

Authors
  1. Jennifer J. York
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. J. Fahey
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

York, J.J., Fahey, K.J. Humoral and cell-mediated immune responses to the glycoproteins of infectious laryngotracheitis herpesvirus. Archives of Virology 115, 289–297 (1990). https://doi.org/10.1007/BF01310537

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01310537

Keywords

Search

Navigation