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Expression of the structural proteins of dengue 2 virus and yellow fever virus by recombinant vaccinia viruses

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Summary

Vaccinia virus recombinants were constructed which contained cDNA sequences encoding the structural region of dengue 2 virus (PR159/S1 strain) or yellow fever virus (17D strain). The flavivirus cDNA sequences were expressed under the control of the vaccinia 7.5k early/late promotor. Cultured cells infected with these recombinants expressed immunologically reactive flavivirus structural proteins, precursor prM and E. These proteins appeared to be cleaved and glycosylated properly since they comigrated with the authentic proteins from dengue 2 virus- and yellow fever virus-infected cells. Mice immunized with the dengue/vaccinia recombinant showed a dengue-specific immune response that included low levels of neutralizing antibodies. Immunization of mice with the yellow fever/vaccinia recombinant was less effective at inducing an immune response to yellow fever virus and in only some of the mice were low titers of neutralizing antibodies produced.

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References

  1. Anderson DJ, Blobel G (1983) Immunoprecipitation of proteins from cell-free translations. Methods Enzymol 96: 111–120

    Google Scholar 

  2. Bancroft WH, Scott RM, Eckels KH, Hoke CH, Simms TE, Jesrani KDT, Summers PL, Dubois DR, Tsoulos D, Russell PK (1984) Dengue virus type 2 vaccine: reactogenicity and immunogenicity in soldiers. J Infect Dis 149: 1005–1010

    Google Scholar 

  3. Bray M, Zhao B, Markoff L, Eckels, KH, Chanock RM, Lai CJ (1989) Mice immunized with recombinant vaccinia virus expressing dengue 4 virus structural proteins with or without nonstructural protein NS1 are protected against fatal dengue virus encephalitis. J Virol 63: 2853–2856

    Google Scholar 

  4. Brinton, MA (1986) Replication of flaviviruses. In: Schlesinger S, Schlesinger M (eds) Togaviridae and Flaviviridae. Plenum, New York, pp 327–365

    Google Scholar 

  5. Cane PA, Gould EA (1988) Reduction of yellow fever virus mouse neurovirulence by immunization with a bacterially synthesized nonstructural protein (NS1) fragment. J Gen Virol 69, 1241–1246

    Google Scholar 

  6. Cardiff RD, Lund JK (1976) Distribution of dengue-2 virus antigens by electron immunocytochemistry. Infect Immun 13: 1699–1709

    Google Scholar 

  7. Cleaves GR (1985) Identification of dengue type 2 virus specific high molecular weight proteins in virus-infectd BHK cells. J Gen Virol 66: 2767–2771

    Google Scholar 

  8. Deubel V, Kinney RM, Esposito JJ, Cropp CB, Vorndam AV, Monath TP, Trent DW (1988) Dengue 2 virus envelope protein expressed by a recombinant vaccinia virus fails to protect monkeys against dengue. J Gen Virol 69: 1921–1929

    Google Scholar 

  9. Eckels KH, Brandt WE, Harrison VR, McCown JH, Russell PK (1976) Isolation of a temperature-sensitive dengue-2 virus under conditions suitable for vaccine development. Infect Immun 14: 1221–1227

    Google Scholar 

  10. Eckels KH, Harrison VR, Summers PL, Russell PK (1980) Dengue-2 vaccine: preparation from a small-plaque virus clone. Infect Immun 27: 175–180

    Google Scholar 

  11. Eckels KH, Scott RMcN, Bancroft WH, Brown J, Dubois DR, Summers PL, Russell PK, Halstead SB (1984) Selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. V. Human response to immunization with a candidate vaccine prepared in fetal rhesus lung cells. Am J Trop Med Hyg 33: 684–689

    Google Scholar 

  12. Elango N, Prince GA, Murphy BR, Venkatesan S, Chanock RM, Moss B (1986) Resistance to human respiratory syncytial virus (RSV) infection induced by immunization of cotton rats with recombinant vaccinia virus expressing the RSV G glycoprotein. Proc Natl Acad Sci USA 83: 1906–1910

    Google Scholar 

  13. Falgout B, Chanock R, Lai CJ (1989) Proper processing of dengue virus nonstructural glycoprotein NS1 requires the N-terminal hydrophobic signal sequence and the downstream nonstructural protein NS2A. J Virol 63: 1852–1860

    Google Scholar 

  14. Gubler DJ (1988) Dengue. In: Monath TP (ed) The arboviruses: epidemiology and ecology, vol II. CRC Press, Boca Raton, FL, pp 223–260

    Google Scholar 

  15. Hahn YS, Galler R, Hunkapiller R, Dalrymple JM, Strauss JH, Strauss EG (1988) Nucleotide sequence of dengue 2 DNA and comparison of the encoded proteins with those of other flaviviruses. Virology 162: 167–180

    Google Scholar 

  16. Halstead SB (1988) Pathogenesis of dengue: challenge to molecular biology. Science 239: 476–481

    Google Scholar 

  17. Halstead SB, Venkateshan CN, Gentry MK, Larsen LK (1984) Heterogeneity of infection enhancement of dengue 2 strains by monoclonal antibodies. J Immunol 132: 1529–1532

    Google Scholar 

  18. Hardy WR, Strauss JH (1988) Processing the nonstructural polyproteins of Sindbis virus: study of the kinetices in vivo using monospecific antibodies. J Virol 62: 998–1007

    Google Scholar 

  19. Heinz FX (1986) Epitope mapping of flavivirus glycoproteins. Adv Virus Res 31: 103–168

    Google Scholar 

  20. Hruby DE, Guarino LA, Kates JR (1979) Vaccinia virus replication. J Virol 29: 705–715

    Google Scholar 

  21. Kinney R, Esposito JJ, Mathews JH, Johnson BJB, Roehrig JT, Barrett ADT, Trent DW (1988) Recombinant vaccinia virus/Venezuelan equine encephalitis (VEE) virus protects mice from peripheral VEE challenge. J Virol 62: 4697–4702

    Google Scholar 

  22. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature 227: 680–685

    Google Scholar 

  23. Mackett M, Smith GL (1986) Vaccinia virus expression vectors. J Gen Virol 67: 2067–2082

    Google Scholar 

  24. Mackett J, Smith GL, Moss B (1984) General method for production and selection of infectious vaccinia recombinants expressing foreign genes. J Virol 49: 857–864

    Google Scholar 

  25. Maniatis T, Fritch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  26. Markoff L (1989) In vitro processing of dengue virus structural proteins: cleavage of the pre-membrane protein. J Virol 63: 3345–3352

    Google Scholar 

  27. Monath TP (1986) Pathology of flaviviruses. In: Schlesinger S, Schlesinger M (eds) The Togaviridae and Flaviviridae. Plenum, New York, pp 375–440

    Google Scholar 

  28. Nowak T, Farber PM, Wengler G, Wengler G (1989) Analyses of the terminal sequences of West Nile virus structural proteins and of the in vitro translation of these proteins allow the proposal of a complete scheme of the proteolytic cleavages involved in their synthesis. Virology 169: 365–376

    Google Scholar 

  29. Rice CM, Dalgarno L, Galler R, Hahn YS, Strauss EG, Strauss JH (1988) Molecular cloning of flavivirus genomes for comparative analysis and expression. In: Bauer H, Klenk H-D, Scholtissek C (eds) Modern trends in virology, proceedings of the international symposium, June 1986, Giessen. Springer, Berlin Heidelberg New York Tokyo, pp 83–97

    Google Scholar 

  30. Rice CM, Franke C, Strauss JH, Hruby DE (1985) Expression of Sindbis structural proteins via recombinant vaccinia virus: synthesis, processing, and incorporation into mature Sindbis virions. J Virol 56: 227–239

    Google Scholar 

  31. Rice CM, Lenches EM, Eddy SR, Shin SJ, Sheets RL, Strauss JH (1985) Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229: 726–733

    Google Scholar 

  32. Rice CM, Strauss EG, Strauss JH (1986) Structure of the flavivirus genome. In: Schlesinger S, Schlesinger M (eds) The Togaviridae and Flaviviridae. Plenum, New York, pp 279–326

    Google Scholar 

  33. Rice CM, Strauss JH (1982) Association of Sindbis virion glycoproteins and their precursors. J Mol Biol 154: 325–348

    Google Scholar 

  34. Ruiz-Linares A, Cahour A, Despres P, Girard M, Bouloy M (1989) Processing of the yellow fever virus polyprotein: role of cellular proteases in maturation of the structural proteins. J Virol 53: 4199–4209

    Google Scholar 

  35. Schlesinger JJ, Brandriss MW, Cropp CB, Monath TP (1986) Protection against yellow fever in monkeys by immunization with yellow fever virus nonstructural protein NS1. J Virol 60: 1153–1155

    Google Scholar 

  36. Schlesinger JJ, Brandriss MW, Monath TP (1983) Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition, and immune precipitation of the virus envelope protein. Virology 125: 8–17

    Google Scholar 

  37. Schlesinger JJ, Brandriss MW, Walsh EE (1985) Protection against 17D yellow fever encephalitis in mice by passive transfer of monoclonal antibodies to the nonstructural glycoprotein gp48 and by active immunization with gp48. J Immunol 135: 2805–2809

    Google Scholar 

  38. Schlesinger JJ, Brandriss MW, Walsh EE (1987) Protection of mice against dengue 2 encephalitis by immunization with dengue 2 virus nonstructural glycoprotein NS1. J Gen Virol 68: 853–857

    Google Scholar 

  39. Shapiro D, Brandt WE, Russell PK (1972) Change involving a viral membrane glycoprotein during morphogenesis of group B arboviruses. Virology 50: 906–911

    Google Scholar 

  40. Smith GL, Murphy BR, Moss B (1983) Construction and characterization of an infectious vaccinia virus recombinant that expressed the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters. Proc Natl Acad Sci USA 80: 7155–7159

    Google Scholar 

  41. Smith GW, Wright PJ (1985) Synthesis of proteins and glycoproteins in the dengue type 2 virus-infected Vero andAedes albopictus cells. J Gen Virol 66: 559–571

    Google Scholar 

  42. Smith TJ, Brandt WE, Swanson JL, McCown JM, Buesher EL (1970) Physical and biological properties of dengue 2 virus and associated antigens. J Virol 5: 524–532

    Google Scholar 

  43. Stevens TM, Schlesinger RW, Schulze IT (1965) Studies on the nature of dengue viruses. I. Correlation of particle density, infectivity and RNA content of type 2 virus. Virology 27: 103–112

    Google Scholar 

  44. Summers PL, Eckels KH, Dalrymple JM, Scott RM, Boyd VA (1984) Antibody response to dengue 2 vaccine measured by two different radioimmunoassay methods. J Clin Microbiol 195: 651–659

    Google Scholar 

  45. Sweet BH, Sabin AB (1954) Properties and antigenic relationships of hemagglutinins associated with the dengue viruses. J Immunol 73: 363–373

    Google Scholar 

  46. Wertz GW, Stott EJ, Young KKY, Anderson K, Ball LA (1987) Expression of the fusion protein of human respiratory syncytial virus from recombinant vaccinia virus vectors and protection of vaccinated mice. J Virol 61: 293–301

    Google Scholar 

  47. Westaway EG (1987) Flavivirus replication strategy. Adv Virus Res 33: 45–90

    Google Scholar 

  48. Zhang Y, Hayes, EP, McCarty TC, Dubois DR, Summers PL, Eckels KH, Chanock RM, Lai C-J (1988) Immunization of mice with dengue structural proteins and nonstructural protein NS1 expressed by a baculovirus recombinant induces resistance to dengue virus encephalitis. J Virol 62: 3027–3031

    Google Scholar 

  49. Zhao B, Prince G, Horswood R, Eckels K, Summers P, Chanock R, Lai C-J (1987) Expression of dengue virus structural proteins and nonstructural protein NS1 by a recombinant vaccinia virus. J Virol 61: 4019–4022

    Google Scholar 

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Author notes

  1. Y. S. Hahn

    Present address: Department of Pathology, Washington University School of Medicine, St. Louis, Missouri, USA

  2. R. Galler

    Present address: Fundacao Oswaldo Cruz, Departmento de Bioquimica e Biologia Molecular, Rio de Janeiro, Brazil

  3. C. M. Rice

    Present address: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, California

    Y. S. Hahn, Edith M. Lenches, R. Galler, C. M. Rice & J. H. Strauss

  2. Virology Division, U.S. Army Medical Research Institute of Infectious Diseases, Ft. Detrick, Frederick, Maryland, USA

    J. Dalrymple

Authors
  1. Y. S. Hahn
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  2. Edith M. Lenches
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  3. R. Galler
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  4. C. M. Rice
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  5. J. Dalrymple
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  6. J. H. Strauss
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Hahn, Y.S., Lenches, E.M., Galler, R. et al. Expression of the structural proteins of dengue 2 virus and yellow fever virus by recombinant vaccinia viruses. Archives of Virology 115, 251–265 (1990). https://doi.org/10.1007/BF01310534

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  • DOI: https://doi.org/10.1007/BF01310534

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