Summary
Comparative immunochemical analysis of different tobamoviruses indicated that the previously reported coat protein sequence of Odontoglossum ringspot virus was likely erroneous. This sequence has been determined again by direct sequencing of the genomic RNA and was found to differ from the previously proposed sequence in 31 of the 157 amino acid residues. The extent of antigenic cross-reactivity between ORSV protein and other tobamovirus proteins was measured by ELISA and found to correlate satisfactorily with the degree of sequence homology.
Similar content being viewed by others
References
Al Moudallal Z, Briand JP, Van Regenmortel MHV (1985) A major part of the polypeptide chain of tobacco mosaic virus protein is antigenic. EMBO J 4: 1231–1235
Altschuh D, Al Moudallal Z, Briand JP, Van Regenmortel MHV (1985) Immunochemical studies of tobacco mosaic virus. VI. Attempts to localize viral epitopes with monoclonal antibodies. Mol Immunol 22: 329–337
Bloomer AC, Champness JN, Bricogne G, Staden R, Klug A (1978) Protein disk of tobacco mosaic virus at 2.8 Å resolution showing the interactions within and between subunits. Nature 276: 362–368
Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the Vax. Nucleic Acids Res 12: 387–395
Dore I, Altschuh D, Al Moudallal Z, Van Regenmortel MHV (1987) Immunochemical studies of tobacco mosaic virus. VII. Use of comparative surface accessibility of residues in antigenically related viruses for delineating epitopes recognized by monoclonal antibodies. Mol Immunol 24: 1351–1358
Dore I, Dekker EL, Porta C, Van Regenmortel MHV (1987) Detection by ELISA of two tobamoviruses in orchids using monoclonal antibodies. J Phytopathol 120: 317–326
Dore I, Weiss E, Altschuh D, Van Regenmortel MHV (1988) Visualization by electron microscopy of the location of tobacco mosaic virus epitopes reacting with monoclonal antibodies in enzyme immunoassay. Virology 162: 279–289
Geliebter J, Zeff RA, Melvold RW, Nathenson SG (1986) Mitotic recombination in germ cells generated two major histocompatibility complex mutant genes shown to be identical by RNA sequence analysis: Kbm9 and Kbm6. Proc Natl Acad Sci USA 83: 3371–3375
Goelet P, Lomonossoff GP, Butler PJG, Akam ME, Gait MJ, Karn J (1982) Nucleotide sequence of tobacco mosaic virus RNA. Proc Natl Acad Sci USA 79: 5818–5822
Hennig B (1970) Die primäre Proteinstruktur von Stämmen des Tabakmosaikvirus: Aminosäurenzusammensetzungen und Partialsequenzen der tryptischen Peptide der Hüllproteine von Odontoglossum Ringspot Virus, Birnen-TMV 1 und Cowpea Virus. Doctoral dissertation, University of Tübingen, Tübingen, Federal Republic of Germany.
Hennig B (1972) Coat protein — tobacco mosaic virus Odontoglossum ringspot strain. In: Dayhoff MO (ed) Atlas of protein sequence and structure, vol 5. National Biomedical Research Foundation, Washington, DC, D-287
Jaeglé M, Van Regenmortel MHV (1985) Use of ELISA for measuring the extent of serological cross-reactivity between plant viruses. J Virol Methods 11: 189–198
Lerner RA (1984) Antibodies of predetermined specificity in biology and medicine. Adv Immunol 36: 1–44
Meshi T, Kiyama R, Ohno T, Okada Y (1983) Nucleotide sequence of the coat protein and the 3′ noncoding region of cucumber green mottle mosaic virus (watermelon strain) RNA. Virology 127: 54–64
Ohno T, Aoyagi M, Yamanashi Y, Saito H, Ikawa S, Meshi T, Okada Y (1984) Nucleotide sequence of the tobacco mosaic virus (tomato strain). Genome and comparison with the common strain genome. J Biochem 96: 1915–1923
Okada Y (1986) Cucumber green mottle mosaic virus. In: Van Regenmortel MHV, Fraenkel H (eds) The rod-shaped plant viruses, vol 2. Plenum, New York, pp 267–281
Paul HL (1975) Odontoglossum ringspot virus. CMI/AAB Descriptions of Plant Viruses, no 155
Paul HL, Wetter C, Wittmann HG, Brandes J (1965) Untersuchungen am Odontoglossum Ringspot Virus, einem Verwandten des Tabakmosaik-Virus. I. Physikalische, chemische, serologische und symptomatologische Befunde. Z Vererbungsl 97: 186–203
Sanger F, Nicklen S, Coulsen AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Van Regenmortel MHV (1975) Antigenic relationship between strains of tobacco mosaic virus. Virology 64: 415–420
Van Regenmortel MHV (1981) Tobamoviruses. In: Kurstak E (ed) Handbook of plant virus infections and comparative diagnosis. Elsevier/North-Holland, Amsterdam, pp 541–564
Van Regenmortel MHV, Fraenkel-Conrat H (eds) (1986) The rod-shaped plant viruses, vol 2. Plenum, New York
Van Regenmortel MHV, (1986) The antigenic structure of TMV. In: Van Regenmortel MHV, Fraenkel-Conrat H (eds) The rod-shaped plant viruses, vol 2. Plenum, New York, pp 79–104
Varma A (1986) Sunn-hemp mosaic virus. In: Van Regenmortel MHV, Fraenkel-Conrat H (eds) The rod-shaped plant viruses, vol 2. Plenum, New York, pp 249–266
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dubs, M.C., Van Regenmortel, M.H.V. Odontoglossum ringspot virus coat protein: sequence and antigenic comparisons with other tobamoviruses. Archives of Virology 115, 239–249 (1990). https://doi.org/10.1007/BF01310533
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01310533