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Inhibition of African swine fever virus in cultured swine monocytes by phosphonoacetic acid (PAA) and by phosphonoformic acid (PFA)

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Summary

The use of phosphonoacetic (PAA) and phosphonoformic acid (PFA) as inhibitors of African swine fever virus (ASFV) replication in porcine monocytes/macrophages (MO) was investigated. At concentrations sufficient to inhibit replication, hemadsorption, and cytopathogenic damage by high inocula of ASFV, both antiviral agents were cytostatic and suppressed the DNA-synthetic growth response of porcine MO to the MO-specific colony-stimulating factor-1 (CSF-1). PAA and PFA inhibited ASFV-associated DNA-synthesis in the cytoplasm of infected swine MO. Using ASFV-specific monoclonal antibodies in immunebinding assays and in immunoprecipitation analysis of radiolabeled proteins of infected MO, PAA and PFA inhibited the synthesis of ASFV proteins of 13, 73, and 150/220 kDa, and caused a variable inhibition in the synthesis of a 12 kDa ASFV protein. These antiviral drugs, however, did not prevent the appearance of an early 32 kDa ASFV protein. The cytostatic and virus-suppressive effects of PAA and PFA could be reversed. ASFV resumed growth in infected MO cultures, if the cells maintained in medium with CSF-1 were removed from the antivirals before 1 week of drug exposure. With prolonged exposure to PAA or PFA (beyond 1 week), ASFV could not be recovered from infected MO cultures.

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  1. F. Villinger

    Present address: Yerkes Regional Primate Research Center, Emory University, Atlanta, Georgia, USA

Authors and Affiliations

  1. Plum Island Animal Disease Center, USDA-ARS, Greenport, New York, U.S.A

    F. Villinger, E. V. Genovesi, D. J. Gerstner, T. C. Whyard & R. C. Knudsen

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  1. F. Villinger
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  2. E. V. Genovesi
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  5. R. C. Knudsen
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Villinger, F., Genovesi, E.V., Gerstner, D.J. et al. Inhibition of African swine fever virus in cultured swine monocytes by phosphonoacetic acid (PAA) and by phosphonoformic acid (PFA). Archives of Virology 115, 163–184 (1990). https://doi.org/10.1007/BF01310528

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  • DOI: https://doi.org/10.1007/BF01310528

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