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Establishment of persistent HIV-1 infection in vitro is accompanied by reduction of NF-κB activity

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Summary

Gel retardation analyses showed that NF-κB binding activity was substantially lower in nuclear extracts of HIV persistently infected HUT78 cells compared to their uninfected parental cells, although rates of total NF-κB protein synthesis were similar in both cell types.

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References

  1. Shaw GM, Hahn BH, Arya SK, Groopman JE, Gallo RC, Wong-Staal F (1984) Molecular characterization of human T-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome. Science 226: 1165–1171

    Google Scholar 

  2. Lifson JD, Feinberg MB, Reyes GR, Rabin L, Banapour B, Chakrabarti S, Moss B, Wong-Staal F, Steimer KS, Engleman EG (1986) Induction of CD4-dependent cell fusion by the HTLV-III/LAV envelope glycoprotein. Nature 323: 725–728

    Google Scholar 

  3. Rosenberg ZF, Fauci AS (1991) Immunopathogenesis of HIV infection. FASEB J 5: 2382–2390

    Google Scholar 

  4. Garcia-Blanco MA, Cullen BR (1991) Molecular basis of latency in pathogenic human viruses. Science 254: 815–820

    Google Scholar 

  5. Haase AT (1986) Pathogenesis of lentivirus infections. Nature 322: 130–136

    Google Scholar 

  6. Cullen BR, Greene WC (1989) Regulatory pathways governing HIV-1 replication. Cell 58: 423–426

    Google Scholar 

  7. Mustafa F, Robinson H (1993) Context-dependent role of human immunodeficiency virus type 1 auxiliary genes in the establishment of chronic virus producers. J Virol 67: 6909–6915

    Google Scholar 

  8. Nabel G, Baltimore D (1987) An inducible transcription factor activates expression of human immunodeficiency virus in T cells. Nature 326: 711–713

    Google Scholar 

  9. Jones KA, Kadonaga JT, Luciw PA, Tjian R (1986) Activation of the AIDS retrovirus promoter by the cellular transcription factor, Spl. Science 232: 755–759

    Google Scholar 

  10. Fisher AG, Feinberg MB, Josephs SF, Harper ME, Marselle LM, Reyes G, Gonda MA, Aldovini A, Debouk C, Gallo RC, Wong-Staal F (1986) The trans-activator gene of HTLV-III is essential for virus replication. Nature 320: 367–371

    Google Scholar 

  11. Kliewer S, Garcia J, Pearson L, Soultanakis E, Dasgupta A, Gaynor R (1989) Multiple transcriptional regulatory domains in the human immunodeficiency virus type 1 long terminal repeat are involved in basal and E1A/E1B-induced promoter activity. J Virol 63: 4616–4625

    Google Scholar 

  12. Horvat RT, Wood C, Josephs SF, Balachandran M (1991) Transactivation of the human immunodeficiency virus promoter by human herpesvirus 6 (HHV-6) strains GS and Z-29 in primary human T lymphocytes and identification of transactivating HHV-6(GS) gene fragments. J Virol 65: 2895–2902

    Google Scholar 

  13. Gendelman HE, Phelps W, Feigenbaum L, Ostrove JM, Adachi A, Howley PM, Khoury G, Ginsberg HS, Martin MA (1986) Trans-activation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses. Proc Natl Acad Sci USA 83: 9759–9763

    Google Scholar 

  14. Baeuerle PA (1991) The inducible transcription activator NF-κB: regulation by distinct protein subunits. Biochim Biophys Acta 1072: 63–80

    Google Scholar 

  15. Bohnlein E, Lowenthal JW, Siekevitz M, Ballard DW, Franza BR, Greene WC (1988) The same inducible nuclear proteins regulate mitogen activation of both the interleukin-2 receptor-alpha gene and type 1 HIV. Cell 53: 827–836

    Google Scholar 

  16. Osborn L, Kunkel S, Nabel G (1989) Tumor necrosis factor α and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor κB. Proc Natl Acad Sci USA 86: 2336–2340

    Google Scholar 

  17. Morrow WJW, Homsy J, Eichberg JW, Krowka J, Pan L, Gaston I, Legg H, Lerche N, Thomas J, Levy JA (1989) Long-term observation of baboons, rhesus monkeys, and chimpanzees inoculated with HIV and given periodic immunosuppressive treatment. AIDS Res Hum Retroviruses 5: 233–245

    Google Scholar 

  18. Li P, Burrell CJ (1992) Synthesis of human immunodeficiency virus DNA in a cell-to-cell transmission model. AIDS Res Hum Retroviruses 8: 253–259

    Google Scholar 

  19. Griffin G, Leung K, Folks TM, Kunkel S, Nabel GJ (1990) NF-κB and HIV. Nature 343: 218–219

    Google Scholar 

  20. Schreiber E, Matthias P, Muller MM, Schaffner W (1989) Rapid detection of octamer binding proteins with ‘mini-extracts’, prepared from a small number of cells. Nucleic Acids Res 17: 6419

    Google Scholar 

  21. Dignam JP, Lebovitz RM, Roeder RG (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 11: 1475–1489

    Google Scholar 

  22. Gimble JG, Duh E, Ostrove JM, Gendelaman HE, Max EE, Rabson AB (1988) Activation of the human immunodeficiency virus long terminal repeat by herpes simplex virus type 1 is associated with induction of a nuclear factor that binds to the NF-κB core enhancer sequence. J Virol 62: 4104–4112

    Google Scholar 

  23. Baldwin JR AS, Azizkhan JC, Jensen ME, Berg AA, Coodly LR (1991) Induction of NF-κB DNA-binding activity during the G0-to-G1 transition in mouse fibroblasts. Mol Cell Biol 10: 4943–4951

    Google Scholar 

  24. Pietroboni GR, Harnett GB, Bucens MR (1989) Centrifugal enhancement of human immunodeficiency virus (HIV) and human herpesvirus type 6 (HHV-6) infection in vitro. J Virol Methods 24: 85–90

    Google Scholar 

  25. Ghosh S, Gifford AM, Riviere LR, Tempst P, Nolan GP, Baltimore D (1990) Cloning of the p50 DNA binding subunit of NF-κB: homology to rel and dorsal. Cell 62: 1019–1029

    Google Scholar 

  26. Kawakami K, Scheidereit C, Roeder RG (1988) Identification and purification of a human immunoglobulin enhancer/binding protein (NF-κB) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro. Proc Natl Acad Sci USA 85: 4700–4704

    Google Scholar 

  27. Baeuerle PA, Baltimore D (1989) A 65-kd subunit of active NF-κB is required for inhibition of NF-κB by I-κB. Genes Dev 3: 1689–1699

    Google Scholar 

  28. Ruben SM, Dillon PJ, Schreck R, Henkel T, Chen C, Maher M, Baeuerle PA, Rosen CA (1991) Isolation of rel-related human cDNA that potentially encodes the 65-kD subunit of NF-κB. Science 251: 1490–1493

    Google Scholar 

  29. Baeuerle PA, Baltimore D (1988) Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-κB transcription factor. Cell 53: 211–217

    Google Scholar 

  30. Ghosh S, Baltimore D (1990) Activation in vitro of NF-κB by phosphorylation of its inhibitor IκB. Nature 344: 678–682

    Google Scholar 

  31. Kieran M, Bland V, Logeat F, Vandekerckhove J, Lottspeich F, Le Bail O, Urban M, Kourilsky P, Baeuerle PA, Israel A (1990) The DNA binding subunit of NF-κB is identical to factor KBF1 and homologous to the rel oncogene product. Cell 62: 1007–1018

    Google Scholar 

  32. Zhang X, Bellett AJD, Tha Hla R, Braithwaite AW, Mullbacher A (1991) Adenovirus type 5 E3 gene products interfere with the expression of the cytolytic T cell immunodominant Ela antigen. Virology 180: 199–206

    Google Scholar 

  33. Riviere Y, Bllank V, Kourilsky P, Israel A (1990) Processing of the precursor of NF-κB by the HIV-1 protease during acute infection. Nature 350: 625–626

    Google Scholar 

  34. Raziuddin, Mikovits JA, Calvert I, Ghosh S, Kung HF, Ruscetti FW (1991) Negative regulation of human immunodeficiency virus type 1 expression in monocytes: role of the 65-kDa plus 50-kDa NF-kappa B dimer. Proc Natl Acad Sci USA 88: 9426–9430

    Google Scholar 

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Authors and Affiliations

  1. Institute of Medical and Veterinary Science, Division of Medical Virology, National Centre for HIV Virology, Adelaide

    X. Zhang, P. Li & C. J. Burrell

  2. Department of Microbiology and Immunology, University of Adelaide, Adelaide, Australia

    C. J. Burrell

Authors
  1. X. Zhang
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  2. P. Li
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  3. C. J. Burrell
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Zhang, X., Li, P. & Burrell, C.J. Establishment of persistent HIV-1 infection in vitro is accompanied by reduction of NF-κB activity. Archives of Virology 138, 169–176 (1994). https://doi.org/10.1007/BF01310048

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  • DOI: https://doi.org/10.1007/BF01310048

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