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Nucleotide sequences and expression inEscherichia coli of the coat protein genes from two strains of melon necrotic spot virus

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Summary

The nucleotide sequences of coat protein (CP) genes of two Japanese strains of melon necrotic spot virus·(MNSV-NH and MNSV-S) were determined. The size of the CP genes of both strains was 1170 nucleotides. Comparisons of the deduced amino acid sequences among MNSV strains showed more than 95% homology, and those among other carmoviruses showed 31–34% homology. cDNAs of MNSV CP genes were cloned into anEscherichia coli expression fusion vector and MNSV-NH and MNSV-S CPs were successfully produced. Furthermore, synthetic oligonucleotide primers were used for differentiating MNSV strains by reverse transcription-polymerase chain reaction method.

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Authors and Affiliations

  1. Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga

    K. Ohshima & N. Sako

  2. Nagasaki Agricultural and Forestry Experiment Station, Isahaya, Nagasaki, Japan

    K. Matsuo

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  1. K. Ohshima
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  2. K. Matsuo
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  3. N. Sako
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Ohshima, K., Matsuo, K. & Sako, N. Nucleotide sequences and expression inEscherichia coli of the coat protein genes from two strains of melon necrotic spot virus. Archives of Virology 138, 149–160 (1994). https://doi.org/10.1007/BF01310046

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  • DOI: https://doi.org/10.1007/BF01310046

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