Archives of Virology

, Volume 138, Issue 1–2, pp 143–148 | Cite as

Passive protection against bovine rotavirus in calves by specific immunoglobulins from chicken egg yolk

  • M. Kuroki
  • M. Ohta
  • Y. Ikemori
  • R. C. Peralta
  • H. Yokoyama
  • Y. Kodama
Brief Report


The efficacy of chicken egg yolk immunoglobulins (yIg) from hens immunized with bovine rotavirus (BRV) serotype G6 (strain Shimane) or serotype G10 (strain KK-3) for protection against homologous BRV in calves was investigated. A significant protection by anti-BRV yIg having 6400 neutralizing antibody titer per dose could be achieved in calves (P<0.01).


Infectious Disease Antibody Titer Neutralize Antibody Significant Protection Neutralize Antibody Titer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Babiuk LA (1990) Viral gastroenteritis in ruminants. In: Dinter Z, Morein B (eds) Virus infections of ruminants. Elsevier, Amsterdam, pp 555–566Google Scholar
  2. 2.
    Bartz CR, Conklin RH, Tunstall CB, Steele JH (1980) Prevention of murine rotavirus infection with chicken egg yolk immunoglobulins. J Infect Dis 142: 439–441Google Scholar
  3. 3.
    Bellinzoni RC, Blackhall J, Baro N, Auza N, Mattion N, Casaro A, La Torre JL, Scodeller EA (1989) Efficacy of an inactivated oil-adjuvanted rotavirus vaccine in the control of calf diarrhoea in beef herds in Argentina. Vaccine 7: 263–268Google Scholar
  4. 4.
    Broome RL, Vo PT, Ward RL, Clark HF, Greenberg HB (1993) Murine rotavirus genes encoding outer capsid proteins VP4 and VP7 are not major determinants of host range restriction and virulence. J Virol 67: 2448–2455Google Scholar
  5. 5.
    Brunser O, Espinoza J, Figueroa G, Araya M, Spencer E, Hilpert H, Link-Amster H, Brüssow H (1992) Field trial of an infant formula containing anti-rotavirus and anti-Escherichia coli milk antibodies from hyperimmunized cows. J Pediatr Gastroenterol Nutr 15: 63–72Google Scholar
  6. 6.
    Cornaglia EM, Fernández FM, Gottschalk M, Barrandeguy ME, Luchelli A, Pasini MI, Saif LJ, Parraud JR, Romat A, Schudel AA (1992) Reduction in morbidity due to diarrhea in nursing beef calves by use of an inactivated oil-adjuvanted rotavirus-Escherichia coli vaccine in the dam. Vet Microbiol 30: 191–202Google Scholar
  7. 7.
    Ebina T, Tsukada K, Umezu K, Nose M, Tsuda K, Hatta H, Kim M, Yamamoto T (1990) Gastroenteritis in suckling mice caused by human rotavirus can be prevented with egg yolk immunoglobulin (IgY) and treated with a protein-bound polysaccharide preparation (PSK). Microbiol Immunol 34: 617–629Google Scholar
  8. 8.
    Fenner FJ, Gibbs EPJ, Murphy FA, Rott R, Studdert MJ, White DO (1993) Diseases caused by rotaviruses. Chapter 31. In: Fenner FJ et al. (eds) Veterinary virology, 2nd edn. Academic Press, San Diego, pp 550–552Google Scholar
  9. 9.
    Fichtali J, Charter EA, Lo KV, Nakai S (1992) Separation of egg yolk immunoglobulins using an automated liquid chromatography system. Biotechnol Bioeng 40: 1388–1394Google Scholar
  10. 10.
    Fukusho A, Shimizu Y, Ito Y (1981) Isolation of cytopathic porcine rotavirus in cell roller culture in the presence of trypsin. Arch Virol 69: 49–60Google Scholar
  11. 11.
    Ikemori Y, Kuroki M, Peralta RC, Yokoyama H, Kodama Y (1992) Protection of neonatal calves against fatal enteric colibacillosis by administration of egg yolk powder from hens immunized with K99-piliated enterotoxigenicEscherichia coli. Am J Vet Res 53: 2005–2008Google Scholar
  12. 12.
    Kuroki M, Ikemori Y, Yokoyama H, Peralta RC, Icatlo Jr FC, Kodama Y (1993) Passive protection against bovine rotavirus-induced diarrhea in murine model by specific immunoglobulins from chicken egg yolk. Vet Microbiol 37: 135–146Google Scholar
  13. 13.
    Matsuda Y, Nakagomi O, Offit PA (1990) Presence of three P types (VP4 serotypes) and two G types (VP7 serotypes) among bovine rotavirus strains. Arch Virol 115: 199–207Google Scholar
  14. 14.
    Murakami Y, Nishioka N, Hashiguchi Y, Kuniyasu C (1983) Serotypes of bovine rotaviruses distinguished by serum neutralization. Infect Immun 40: 851–855Google Scholar
  15. 15.
    Sato K, Inaba Y, Takahashi E, Ito Y, Kurogi H, Akashi H, Satoda K, Omori T, Matumoto M (1978) Isolation of a reovirus-like agent (rotavirus) from neonatal calf diarrhea in Japan. Microbiol Immunol 22: 499–503Google Scholar
  16. 16.
    Snodgrass DR, Fitzgerald T, Campbell I, Scott FMM, Browning GF, Miller DL, Herring AJ, Greenberg HB (1990) Rotavirus serotypes 6 and 10 predominate in cattle. J Clin Microbiol 28: 504–507Google Scholar
  17. 17.
    Suzuki Y, Sanekata T, Sato M, Tajima K, Matsuda Y, Nakagomi O (1993) Relative frequencies of G (VP7) and P (VP4) serotypes determined by polymerase chain reaction assays among Japanese bovine rotaviruses isolated in cell culture. J Clin Microbiol 31: 3046–3049Google Scholar
  18. 18.
    Theil KW (1990) Group A rotaviruses. In: Saif LJ, Theil KW (eds) Viral diarrheas of man and animals. CRC Press, Boca Raton, pp 35–72Google Scholar
  19. 19.
    Tsunemitsu H, Shimizu M, Hirai T, Yonemichi H, Kudo T, Mori K, Onoe S (1989) Protection against bovine rotaviruses in newborn calves by continuous feeding of immune colostrum. Jpn J Vet Sci 51: 300–308Google Scholar
  20. 20.
    Yokoyama H, Peralta RC, Horikoshi T, Hiraoka J, Ikemori Y, Kuroki M, Kodama Y (1993) A two-step procedure for purification of hen egg yolk immunoglobulin G: utilization of hydroxypropylmethylcellulose phthalate and synthetic affinity ligand gel (Avid AL®). Poult Sci 72: 275–281Google Scholar
  21. 21.
    Yolken RH, Leister F, Wee S-B, Miskuff R, Vonderfecht S (1988) Antibodies to rotaviruses in chickens' eggs: a potential source of antiviral immunoglobulins suitable for human consumption. Pediatrics 81: 291–295Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. Kuroki
    • 1
  • M. Ohta
    • 1
  • Y. Ikemori
    • 1
  • R. C. Peralta
    • 1
  • H. Yokoyama
    • 1
  • Y. Kodama
    • 1
  1. 1.Immunology Research Institute in GifuSano, Gifu-CityJapan

Personalised recommendations