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Expression of protein kinase A and protein kinase C during ongoing human cytomegalovirus infection

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Summary

During an HCMV infection, transcription of viral and cellular genes are mutually regulated. Several cellular proteins have been implicated in the regulation of the HCMV major immediate early promoter (MIEP) which have been shown to respond to cAMP as well as activation of protein kinase C (PKC). We have examined the effect of an ongoing HCMV infection at the mRNA level for the catalytic and regulatory subunits of protein kinase A (PKA) and α and β isoforms of PKC. There was a moderate elevation for PKA Cα and RIα at immediate early times (0.5–2 h) after HCMV infection. Later in the infection cycle (24–72 h), mRNA level for PKA regulatory subunit RIα and PKCα were decreased compared with control cells. Messenger RNA levels for the PKA RIIα and RIIβ as well as PKCβ were not affected by HCMV infection. During the infection cycle the PKA subunits and PKC isoforms appeared to be independently regulated. It was also evident that the basal mRNA levels of PKA subunits and the PKC isoforms were sufficient for the PKA and PKC activity required during an HCMV infection in permissive fibroblast cells.

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Authors and Affiliations

  1. Institute of Bacteriology, University of Oslo, Oslo, Norway

    A. K. Kristoffersen & H. Rollag

  2. Institute of Medical Biochemistry, University of Oslo, Oslo, Norway

    K. Taskèn

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  1. A. K. Kristoffersen
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  2. K. Taskèn
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  3. H. Rollag
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Kristoffersen, A.K., Taskèn, K. & Rollag, H. Expression of protein kinase A and protein kinase C during ongoing human cytomegalovirus infection. Archives of Virology 138, 85–93 (1994). https://doi.org/10.1007/BF01310040

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  • DOI: https://doi.org/10.1007/BF01310040

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