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Inhibition of the herpes simplex virus-coded thymidine kinase-complex by 9-β-D-arabinofuranosyladenine 5′-monophosphate (ara-AMP) and 9-(2-hydroxyethoxymethyl)guanine-monophosphate (acyclo-GMP)

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Summary

The thymidine kinase-complex isolated from herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2) is associated with the following enzyme activities: ATP:dThd (dCyd) deoxypyrimidine kinase, ATP:dTMP thymidylate kinase, ADP:dThd- and AMP:dThd5′-phosphotransferase. In kinetic experiments it is shown that ara-AMP inhibits AMP:dThd- and ADP:dThd phosphotransferase activity, while acyclo-GMP impairs ADP:dThd phosphotransferase reaction only; the inhibition was found to be non-compertitive. The functional subunit ATP:dThd kinase was not affected by either compound.

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Authors and Affiliations

  1. Abteilung für Experimentelle Virologie, Institut für Medizinische Mikrobiologie, Germany

    J. Labenz & D. Falke

  2. Institut für Physiologische Chemie, Abteilung für Angewandte Molekularbiologie, Johannes-Gutenberg-Universität, Mainz, Germany

    W. E. G. Müller

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  1. J. Labenz
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  2. W. E. G. Müller
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  3. D. Falke
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Labenz, J., Müller, W.E.G. & Falke, D. Inhibition of the herpes simplex virus-coded thymidine kinase-complex by 9-β-D-arabinofuranosyladenine 5′-monophosphate (ara-AMP) and 9-(2-hydroxyethoxymethyl)guanine-monophosphate (acyclo-GMP). Archives of Virology 81, 205–212 (1984). https://doi.org/10.1007/BF01309993

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  • DOI: https://doi.org/10.1007/BF01309993

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