Summary
Segment 10, encoding nonstructural proteins 3 (NS3) and 3a (NS3a) of epizootic haemorrhagic disease virus serotype 1 (EHDV-1) was sequenced. Computer motif recognition programs were used for interpretation of the sequence data to predict a structure for NS3. Integral membrane protein theories were then applied to produce a general topological model for the EHDV-1 NS3 protein. Homology was observed between EHDV-1 NS3 integral membrane motifs and those similarly observed in the cognate proteins of other orbiviruses.
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References
Chalmers GA, Vance HN, Mitchell GJ (1964) An outbreak of epizootic hemorrhagic disease virus in wild ungulates in Alberta. J Wildl Dis 42: 1–6
Wilson WC (1994) Sequence analysis of the nonstructural protein 2 from epizootic haemorrhagic disease viruses. Virus Res 34: 63–68
Wilson WC (1993) Development of a nested-PCR test based on sequence analysis of epizootic haemorrhagic disease viruses non-structural protein 1 (NS1). Virus Res 31: 357–365
Jensen MJ, Cheney IW, Thompson LH, Mecham JO, Wilson WC, Yamakawa M, Roy P, Gorman BM (1994) The smallest gene of the orbivirus, epizootic haemorrhagic disease, is expressed in virus-infected cells as two proteins and the expression differs from that of the cognate gene of bluetongue virus. Virus Res 32: 353–364
Mecham JO, Dean VC (1988) Protein coding assignment for the genome of epizootic haemorrhagic disease virus. J Gen Virol 69: 1255–1262
Mertens PPC, Brown F, Sangar DV (1984) Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology 135: 207–217
Roy P, Marshall JJ, French TJ (1990) Structure of the bluetongue virus genome and its encoded proteins. Curr Top Microbiol Immunol 162: 1–19
Wu X, Chen SY, Iwata H, Compans R, Roy P (1992) Multiple glycoproteins synthesized by the smallest RNA segment (S10) of bluetongue virus. J Virol 66: 7104–7112
Hyatt AD, Zhao Y, Roy P (1993) Release of virus-like particles from insect cells is mediated by BTV nonstructural protein NS3-3A. Virology 193: 592–603
Gould AR (1988) Nucleotide sequence of the Australian bluetongue virus serotype 1 RNA segment 10. J Gen Virol 69: 945–949
Van Staden V, Huismans H (1991) A comparison of the genes which encode non-structural protein NS3 of different orbiviruses. J Gen Virol 72: 1073–1079
von Heijne G (1990) The signal peptide. J Membr Biol 115: 195–201
Wilson WC, Fukusho A, Roy P (1990) Diagnostic complementary DNA probes for genome segments 2 and 3 of epizootic haemorrhagic disease virus serotype 1. Am J Vet Res 51: 855–860
Meyers EW, Miller W (1988) Description of the alignment method used in program PALIGN. CABIOS 4: 11–17
Eisenberg D, Schwarz E, Komaromy M, Wall R (1984) Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J Mol Biol 179: 125–142
Eisenberg D, Weiss RM, Terwilliger TC (1982) The helical hydrophobic moment: a measure of the amphiphilicity of a helix. Nature 299: 371–374
Schiffer M, Edmondson AB (1967) Use of helical wheels to represent the structures of proteins and to identify segments with helical potential. Biophys J 7: 121–135
von Heijne G (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 14: 4683–4690
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Jensen, M.J., Wilson, W.C. A model for the membrane topology of the NS3 protein as predicted from the sequence of segment 10 of epizootic haemorrhagic disease virus serotype 1. Archives of Virology 140, 799–805 (1995). https://doi.org/10.1007/BF01309968
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DOI: https://doi.org/10.1007/BF01309968