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Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model

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Summary

The glycoprotein C (gC) gene of equine herpesvirus-1 (EHV-1) was expressed in insect cells by a recombinant baculovirus as several products with apparent molecular weights of 66 kDa–80 kDa. The baculovirus EHV-1 gC products were recognised by monoclonal antibody and by EHV-1 convalescent equine sera, indicating conservation of antigenic determinants and confirming this glycoprotein as a target for the equine immune system. Mice immunized with recombinant EHV-1 gC showed accelerated clearance of EHV-1 from respiratory tissues following intranasal challenge. Virus clearance was accompanied by virus specific antibodies and by cell mediated immune responses measured by a delayed type hypersensitivity reaction and lymphocyte stimulation by killed EHV-1 as antigen.

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Authors and Affiliations

  1. Department of Veterinary Medicine, University of Cambridge, Cambridge, U.K.

    D. Tewari & H. J. Field

  2. School of Biological Sciences, Macquarie University, Sydney

    S. V. Nair, M. C. De Ungria, G. L. Lawrence & J. M. Whalley

  3. CSL Ltd., Parkville

    M. Hayden

  4. Department of Veterinary Pathology, University of Sydney, Sydney, Australia

    D. N. Love

Authors
  1. D. Tewari
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  2. S. V. Nair
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  3. M. C. De Ungria
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  4. G. L. Lawrence
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  5. M. Hayden
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  6. D. N. Love
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  7. H. J. Field
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  8. J. M. Whalley
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Tewari, D., Nair, S.V., De Ungria, M.C. et al. Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model. Archives of Virology 140, 789–797 (1995). https://doi.org/10.1007/BF01309967

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  • DOI: https://doi.org/10.1007/BF01309967

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