Summary
Herpes simplex virus (HSV) hyperresistant neuroblastoma cells (clone Cl300 RII) were latently infected with HSV-1 if cultured in presence of HSV-neutralizing antibody for one or two passages after infection and then passaged further without antibody. By superinfecting HSV-1 latently infected Cl300 RII cells with HSV-2, progeny virus with HSV-1 characteristics was regularly rescued. Such retrieval of HSV-1 decreased with passage of the latently infected cells.
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References
Augusti-Tocco, Sato, G.: Establishment of functional clonal lines of neurons from mouse neuroblastoma. Proc. Natl. Acad. Sci. U.S.A.64, 311–315 (1969).
Brown, S. M., Subak-Sharpe, J. H., Warren, K. G., Wroblewska, Z., Koprowski, H.: Detection by complementation of defective or uninducible (herpes simplex type 1) virus genomes latent in human ganglia. Proc. Natl. Acad. Sci. U.S.A.76, 2364–2368 (1979).
Colberg-Poley, A. M., Isom, H. C., Rapp, F.: Reactivation of herpes simplex virus type 2 from a quiescent state by human cytomegalovirus. Proc. Natl. Acad. Sci. U.S.A.76, 5948–5951 (1979).
Colberg-Poley, A. M., Isom, H. C., Rapp, F.: Involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2. J. Virol.37, 1051–1059 (1981).
Herschman, H. R.: In:Marks, N., Rodnight, R. (eds.): Research methods in neurochemistry, Vol. 2, 101. New York: Plenum 1974.
Lundén, R., Vahlne, A., Lycke, E.: Measles virus infection of mouse neuroblastoma (Cl300) cells. Proc. Soc. Exp. Biol. Med.165, 55–62 (1980).
Nilheden, E., Jeansson, S., Vahlne, A.: Typing of herpes simplex virus by an enzyme-linked immunosorbent assay with monoclonal antibodies. J. Clin. Microbiol.17, 677–680 (1983).
Nilheden, E., Jeansson, S., Vahlne, A.: Amplification of herpes simplex virus resistance in mouse neuroblastoma (Cl300) cells. Arch. Virol.83, 269–283 (1985).
Park, M., Lonsdale, D. M., Timbary, M. C., Subak-Sharpe, J. H., Macnab, J. C. M.: Genetic restrieval of viral genome sequences from herpes simplex virus transformed cells. Nature285, 412–415 (1980).
Vahlne, A., Lycke, E.: Herpes simplex virus infection of mouse neuroblastoma cells. Proc. Soc. Exp. Biol. Med.156, 82–87 (1977).
Vahlne, A., Nilheden, E., Svennerholm, B.: Multiplicity activation of herpes simplex virus in mouse neuroblastoma (Cl300) cells. Arch. Virol.70, 345–356 (1981).
Wigdahl, B. L., Isom, H. C., de Clercq, E., Rapp, F.: Activation of herpes simplex virus (HSV) type 1 genome by temperature-sensitive mutants of HSV type 2. Virology116, 468–479 (1982).
Wigdahl, B. L., Scheck, A. C., de Clercq, E., Rapp, F.: High efficiency latency and activation of herpes simplex virus in human cells. Science217, 1145–1146 (1982).
Wigdahl, B. L., Ziegler, R. J., Sneve, M., Rapp, F.: Herpes simplex virus latency and reactivation in isolated rat sensory neurons. Virology127, 159–167 (1983).
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Nilheden, E., Jeansson, S. & Vahlne, A. Herpes simplex virus latency in a hyperresistant clone of mouse neuroblastoma (Cl300) cells. Archives of Virology 83, 319–325 (1985). https://doi.org/10.1007/BF01309927
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DOI: https://doi.org/10.1007/BF01309927