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Binding of neutralizing monoclonal antibodies to empty capsids of poliovirus can be blocked by monospecific antisera to structural polypeptides VP1 and VP2

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Summary

Binding of two neutralizing monoclonal antibodies (Nt-mAbs) to natural empty capsids (NEC) of poliovirus, type 1, was blocked to the extent of 83 per cent to 98 per cent by monospecific rabbit antisera directed against the structural polypeptides VP1 and VP2. Monospecific antisera against VP3 or VP4, however, did not show this blocking effect. It is therefore assumed that VP1 and VP2 are located close together at the antigenic sites for the two mAbs.

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References

  1. Blondel, B., Akacem, O., Crainic, R., Couillin, P., Horodniceanu, F.: Detection by monoclonal antibodies of an antigenic determinant critical for poliovirus neutralization present on VP1 and on heat-inactivated virions. Virology126, 707–710 (1983).

    Google Scholar 

  2. Dernick, R., Heukeshoven, J., Hilbrig, M.: Induction of neutralizing antibodies by all three structural poliovirus polypeptides. Virology130, 243–246 (1983).

    Google Scholar 

  3. Drzeniek, R., Bilello, P.: Absence of glycoproteins in poliovirus particles. J. gen. Virol.25, 125–132 (1974).

    Google Scholar 

  4. Emini, E. A., Jameson, B. A., Lewis, A. J., Larsen, G. R., Wimmer, E.: Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies. J. Virol.43, 997–1005 (1982).

    Google Scholar 

  5. Engvall, E., Perlmann, P.: Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. J. Immunol.109, 129–135 (1972).

    Google Scholar 

  6. Evans, D. M. A., Minor, P. D., Schild, G. S., Almond, J. W.: Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3. Nature304, 459–462 (1983).

    Google Scholar 

  7. Haardt, F.: Diplomarbeit Fachbereich Biologie, Universität Hamburg (1982).

  8. Hammarstroem, S., Engvall, E., Johannson, B. G., Svensson, S., Sundblad, G., Goldstein, I. J.: Nature of the tumor-associated determinant(s) of carcinoembryonic antigen. Proc. Natl. Acad. Sci. U.S.A.72, 1528–1532 (1975).

    Google Scholar 

  9. Hawkes, R., Niday, E., Gordon, J.: A dot-immunobinding assay for monoclonal and other antibodies. Anal. Biochem.119, 142–147 (1982).

    Google Scholar 

  10. Heukeshoven, J., Dernick, R.: Chemische Analyse und Struktur des Poliovirus. I. Cystein/Cystin-Gehalt, vollständige Aminosäureanalyse und Hydrophobizität von Poliovirus und seinen natürlichen leeren Kapsiden. Z. Naturforsch.36, 164–172 (1981).

    Google Scholar 

  11. Hummeler, K., Anderson, T. F., Brown, R. A.: Identification of poliovirus particles of different antigenicity by specific agglutination as seen in the electron microscope. Virology16, 84–90 (1962).

    Google Scholar 

  12. Icenogle, J., Gilbert, S. F., Grieves, J., Anderegg, J., Rueckert, R. R.: A neutralizing monoclonal antibody against poliovirus and its reaction with related antigens. Virology115, 211–215 (1981).

    Google Scholar 

  13. Katze, M. G., Crowell, R. L.: Indirect enzyme-linked immunosorbent assay (ELISA) for the detection of Coxsackie group B antibodies. J. gen. Virol.48, 225–229 (1980).

    Google Scholar 

  14. Kennett, R. H., Denis, K. A., Tung, A. S., Klinman, N. R.: In:Melchers, F., Potter, M., Warner, N. (eds.), Lymphocyte Hybridomas. Second Workshop on Functional Properties of Tumors of T and B Lymphocytes, Vol. 81, 77–91. Berlin-Heidelberg-New York: Springer 1978.

    Google Scholar 

  15. Massey, R. J., Schochetman, G.: Topographical analysis of viral epitopes using monoclonal antibodies: mechanism of neutralization. Virology115, 20–32 (1981).

    Google Scholar 

  16. Meloen, R. H., Rowlands, D. J., Brown, F.: Comparison of the antibodies elicited by the individual structural polypeptides of foot-and-mouth disease and polioviruses. J. gen. Virol.45, 761–763 (1979).

    Google Scholar 

  17. Minor, P. D., Schild, G. C., Bootman, J., Evans, D. M. A., Ferguson, M., Reeve, P., Spitz, M., Stanway, G., Cann, A. J., Hauptmann, R., Clarke, L. D., Mountford, R. C., Almond, J. W.: Location and primary structure of a major antigenic site for poliovirus neutralization. Nature301, 674–679 (1983).

    Google Scholar 

  18. Putnak, J. R., Phillips, B. A.: Differences between empty capsids formedin vivo and those formedin vitro: a role for the morphopoietic factor. J. Virol.40, 173–183 (1981).

    Google Scholar 

  19. Putnak, J. R., Phillips, B. A.: Poliovirus empty capsid morphogenesis: evidence for conformational differences between self- and extract-assembled empty capsids. J. Virol.41, 792–800 (1982).

    Google Scholar 

  20. Reggiardo, Z., Vazques, E., Schnaper, L.: ELISA tests for antibodies against mycobacterial glycolipids. J. Immunol. Meth.34, 55–60 (1980).

    Google Scholar 

  21. Rombaut, B., Vrijsen, R., Brioen, P., Boeye, A.: A pH-dependent antigenic conversion of empty capsids of poliovirus studied with the aid of monoclonal antibodies to N and H antigen. Virology122, 215–218 (1982).

    Google Scholar 

  22. Sharff, M. D., Levintow, L.: Quantitative study of the formation of poliovirus antigens in infected HeLa cells. Virology19, 491–500 (1963).

    Google Scholar 

  23. Stone, M. R., Nowinski, R. C.: Topological mapping of murine leukemia virus proteins by competition binding assay with monoclonal antibodies. Virology100, 370–381 (1980).

    Google Scholar 

  24. Taniguchi, K., Urasawa, S., Urasawa, T.: Monoclonal antibodies reactive with different sites of N and H antigenic particles of poliovirus. J. gen. Virol.64, 2803–2808 (1983).

    Google Scholar 

  25. Uhlig, H., Rutter, G., Dernick, R.: Evidence for several unrelated neutralization epitopes of poliovirus type 1, strain Mahoney, provided by neutralization tests and quantitative enzyme-linked immunosorbent assay (ELISA). J. gen. Virol.64, 2809–2812 (1983).

    Google Scholar 

  26. Uhlig, H., Rutter, G., Dernick, R.: Monoclonal antibodies reveal in blocking reactions the antigenicity and antibody response of poliovirus. In: Monoclonal Antibodies, Standardization of Their Characterization and Use. (Developments in Biological Standardization.) Basel: Karger (in press) 1984.

    Google Scholar 

  27. Wiegers, K. J., Drzeniek, R.: Preparative isoelectric focusing of poliovirus polypeptides in urea-sucrose gradients. J. gen. Virol.47, 423–430 (1980).

    Google Scholar 

  28. Wiegers, K. J., Dernick, R.: Monospecific antisera against capsid polypeptides of poliovirus type 1 distinguish antigenic structures of poliovirus proteins. J. gen. Virol.64, 777–785 (1983).

    Google Scholar 

  29. Yamaguchi-Koll, U., Wiegers, K. J., Drzeniek, R.: Isolation and characterization of ‘dense particles’ from poliovirus-infected HeLa cells. J. gen. Virol.26, 307–319 (1975).

    Google Scholar 

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Author notes

  1. Frauke Haardt

    Present address: Physiologisch-chemisches Institut, Universität Hamburg, Martinistrasse 52, D-2000, Hamburg 20, Germany

Authors and Affiliations

  1. Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Hamburg, Germany

    H. Uhlig, Frauke Haardt & R. Dernick

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  1. H. Uhlig
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  2. Frauke Haardt
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  3. R. Dernick
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Uhlig, H., Haardt, F. & Dernick, R. Binding of neutralizing monoclonal antibodies to empty capsids of poliovirus can be blocked by monospecific antisera to structural polypeptides VP1 and VP2. Archives of Virology 83, 295–303 (1985). https://doi.org/10.1007/BF01309924

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  • DOI: https://doi.org/10.1007/BF01309924

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