Summary
The selective proton NMR relaxation rates were measured in the system made by the local anesthetic procaine, human erythrocytes and the hemagglutinating Echo virus type 11. The NMR technique provided a very good tool for using drugs firmly bound to receptor membranes as biological “markers” of the virus-cell interaction.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Rodriguez Boulan, E., Pendergast, M.: Polarized distribution of viral envelope proteins in the plasma membrane of infected epithelial cells. Cell20, 45–54 (1980).
Dubois-Dalq, M., Rentier, B.: Structural studies of the surface of virus-infected cells. Progr. Med. Virol.26, 158–213 (1980).
Wyke, A. M., Impraim, C. C., Knutton, S., Pasternak, C. A.: Components involved in virally mediated membrane fusion and permeability changes. Biochem. J.190, 625–638 (1980).
Pinkett, M. O., Anderson, W. B.: Alterations in plasma membrane proteins associated with the proteolytic activation of adenylate cyclase. Arch. Biochem. Biophys.200, 261–268 (1980).
Rubenstein, E.: Diseases caused by impaired communication among cells. Sci. Amer.242, 78–87 (1980).
McNutt, N. S., Hoffstein, S.: Membranes and cytoskeleton: role in pathologic processes. Fed. Proc.40, 206–213 (1981).
Carrasco, L.: Membrane leakiness after viral infection and a new approach to the development of antiviral agents. Nature272, 694–699 (1978).
Foster, K. A., Gill, K., Micklem, K. J., Pasternak, C. A.: Survey of virally mediated permeability changes. Biochem. J.190, 639–646 (1980).
Impraim, C. C., Foster, K. A., Micklem, K. J., Pasternak, C. A.: Nature of virally mediated changes in membrane permeability to small molecules. Biochem. J.186, 847–860 (1980).
Valensin, G., Gaggelli, E., Tiezzi, E., Valensin, P. E., Bianchi Bandinelli, M. L.: The water proton spin-lattice relaxation rates in virus-infected cells. Biophys. Chem.10, 143–146 (1979).
Valensin, P. E., Bianchi Bandinelli, M. L., Gaggelli, E., Tiezzi, E., Valensin G.: Recognition of early virus-induced changes in cell cultures by Nuclear Magnetic Resonance. Microbiologica3, 25–34 (1980).
Valensin, P. E., Bianchi Bandinelli, M. L., di Cairano, M. L., Valensin, G., Gaggelli, E., Tiezzi, E.: Proton spin-lattice relaxation rates in erythrocytes adsorbed with hemagglutinating viruses. Biophys. Chem.14, 357–362 (1981).
Valensin, G., Gaggelli, E., Tiezzi, E., Valensin, P. E., Bianchi Bandinelli, M. L., di Cairano, M. L.: Virus-cell interactions: Nuclear Magnetic Resonance behavior of intracellular water. Microbiologica5, 195–205 (1982).
Lee, A. G.: Model for action of local anaesthetics. Nature262, 545–548 (1976).
Lee, A. G.: Effects of charged drugs on the phase transition temperatures of phospholipid bilayers. Biochim. Biophys. Acta514, 95–104 (1978).
Pang, K.-Y., Chang, T.-L., Miller, K. W.: On the coupling between anesthetic induced membrane fluidization and cation permeability in lipid vesicles. Mol. Pharmacol.15, 729–738 (1979).
Boulanger, Y., Schreier, S., Leitch, L. C., Smith, I. C. P.: Multiple binding sites for local anesthetics in membranes: characterization of the sites and their equilibria by deuterium NMR of specifically deuterated procaine and tetracaine. Can. J. Biochem.58, 986–995 (1980).
Voeikov, V. L., Lefkowitz, R. J.: Effects of local anesthetics on guanyl nucleotide modulation of the catecholamine-sensitive adenylate cyclase system and on β-adrenergic receptors. Biochim. Biophys. Acta629, 266–281 (1980).
Ayad, S. R., Morgan, S. L.: The effect of temperature and the local anaesthetics, tetracaine, on prostaglandin E1 stimulation of cAMP in normal and malignant cell lines. Exp. Cell Res.109, 9–14 (1977).
Jain, M. K., Wagner, R. C.: Introduction to biological membranes. New York: Wiley & Sons 1980.
Valensin, G., Casini, A., Lepri, A., Gaggelli, E.: Conformational features of cell-bound drugs as detected by selective proton relaxation rate investigations. Biophys. Chem.17, 297–300 (1983).
Nuzzo, F., de Carli, L., Rita, G., Russi, M., Fischer-Fantuzzi, L.: Osservazioni su un caso di relativa stabilità del corredo cromosomico in un ceppo di rene di scimmia in coltura continua. Atti Assoc. Genet. Ital.8, 111–123 (1963).
Dodge, J. T., Mitchell, C., Hanahan, D. J.: The preparation and chemical characteristics of Hemoglobin-free ghosts of human erythrocytes. Arch. Biochem. Biophys.100, 119–130 (1963).
Kohn, A.: Early interactions of viruses with cellular membranes. Adv. Virus Res.24, 223–276 (1979).
Hauser, H., Penkett, S. A., Chapman, D.: Nuclear Magnetic Resonance spectroscopic studies of procaine hydrochloride and tertacaine hydrochloride at lipiwater interfaces. Biochim. Biophys. Acta183, 466–475 (1969).
Author information
Authors and Affiliations
Additional information
With 3 Figures
Rights and permissions
About this article
Cite this article
Valensin, P.E., Bianchi-Bandinelli, M.L., di Cairano, M.L. et al. NMR parameters of local anesthetics as biological markers of the cell-virus interactions. Archives of Virology 83, 241–249 (1985). https://doi.org/10.1007/BF01309920
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309920