Summary
Four influenza A-H3N2 viruses isolated in pigs from different herds in Central Italy in the period 1981/82 have been antigenically and biochemically analysed. Three of them A/Sw/Italy/2/81, A/Sw/Italy/7/81, A/Sw/Italy/8/82 were found to be serologically related to A/Bangkok/1/79 (H3N2). These three viruses were shown to have an identical electrophoretic pattern, as regards virus induced polypeptides and were clearly distinguishable from the virus A/Sw/Italy/6/81 which was antigenically related to A/England/42/72 (H3N2) and A/Sw/Taiwan/7310/70 as shown by specific monoclonal and polyclonal antisera.
The observed biochemical variations underline the importance of the changes occurring by genetic reassortments or mutations in human influenza viruses, during their maintenance in pigs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Advanced Laboratory Techniques for Influenza Diagnosis. Atlanta: Department Health Education and Welfare 1975.
Chapman, M. S., Lamort, P. H., Harkuess, J. W.: Serological evidence of continuing infection of swine in Great Britain with A virus (H3N2). J. Hyg. Camb.80, 415–422 (1978).
de Jong, W. W., Lweers, A., Cohen, L. H.: Influence of single amino acid substitutions on electrophoretic mobility of sodium dodecyl sulfate-protein complexes. Biochem. Biophys. Res. Commun.83, 532–539 (1978).
Hinshaw, V. S., Bean, W. J., Webster, R. G., Easterday, B. C.: The prevalence of influenza viruses in swine and antigenic and genetic relatedness of influenza viruses from man and swine. Virology84, 51–62 (1978).
Hugentobler, A. L., Schild, G. C., Oxford, J. S.: Differences in the electrophoretic migration rates of polypeptides and RNAs of recent isolates of B viruses. Arch. Virol.69, 197–207 (1981).
Inglis, S. C., Carrol, A. R., Lamv, R. A., Mahy, B. W. J.: Polypeptides specified by the influenza virus genome. I Evidence for eight distinct gene products specified by fowl plague virus. Virology74, 489–503 (1976).
Kamar, C., Suwicha, K., Nadhirat, S., Nerome, K., Nakayama, M., Oya, A.: Isolation and serological characterization of influenza A virus from a pig in Thailand. Japan J. Med. Sci. Biol.34, 175–178 (1981).
Kundin, W. D.: Hong Kong A2 influenza virus infection among swine during a human epidemic in Taiwan. Nature (London)228, 957–958 (1970).
Kundin, W. D.:Easterday, B. C.: Hong Kong influenza infection in swine. Experimental and field observations. Bull. W.H.O.47, 489–491 (1972).
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227, 680–685 (1970).
Ottis, K., Bachmann, P. A.: Occurrence of Hsw 1N1 Subtype Influenza A Viruses in Wild Ducks in Europe. Arch. Virol.63, 185–190 (1980).
Ottis, K., Sidoli, L., Bachmann, P. A., Webster, R. G., Kaplan, M. M.: Human influenza A Viruses in Pigs: Isolation of a H3N2 strain antigenically related to A/England/42/72 and evidence for continuous circulation of human viruses in the pig population. Arch. Virol.73, 103–108 (1982).
Oxford, J. S., McGeoch, D. J., Schild, G. C., Beare, A. S.: Analysis of virion RNA segments and polypeptides of influenza A virus recombinants of defined virulence. Nature273, 778–779 (1978).
Oxford, J. S., Corcoran, T., Schild, G. C.: Naturally occurring temperature-sensitive Influenza A Viruses of the H1N1 and H3N2 Subtypes. J. Gen. Virol.48, 383–389 (1980).
Oxford, J. S., Schild, G. C., Alexandrova, G.: Electrophoretic migration rate differences of polypeptides of Human Influenza A Viruses: partial analysis of the genome of influenza vaccine recombinant viruses. Arch. Virol.65, 277–291 (1980).
Oxford, J. S., Corcoran, T., Schild, G. C.: Intratypic electrophoretic variation of structural and non-structural polypeptides of human influenza A viruses. J. Gen. Virol.56, 431–436 (1981).
Oxford, J. S., Corcoran, T., Hugentobler, L.: Quantitative analysis of the protein composition of influenza A and B viruses using high resolution SDS polyacrylamide gels. J. Biol. Stand.9, 483–491 (1981).
Shortridge, K. F., Webster, R. G., Butterfield, W. K., Campbell, C. H.: Persistence of Hong Kong influenza virus variant in pigs. Science196, 1454–1455 (1976).
Shortridge, K. F., Webster, R. G.: Geographical distribution of swine (Hsw 1N1) and Hong Kong (H3N2) influenza variants in pigs in South East Asia. Intervirology11, 9–15 (1979).
Skehel, J. J.: Polypeptides Synthesis in Influenza Virus-Infected Cells. Virology49, 23–36 (1972).
White, D. O.: Current Topics in Microbiology and Immunology6, 63 (1974).
Author information
Authors and Affiliations
Additional information
With 3 Figures
Rights and permissions
About this article
Cite this article
Mancini, G., Donatelli, I., Rozera, C. et al. Antigenic and biochemical analysis of influenza “A” H3N2 viruses isolated from pigs. Archives of Virology 83, 157–167 (1985). https://doi.org/10.1007/BF01309913
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309913