Summary
In preliminary studies, a system for the propagation of respiratory syncytial virus (RSV) using cytodex 3 microcarriers was established. Optimal growth conditions were defined in culture to be a microcarrier concentration of 1.5 g/L with a cell inoculum density of 4 × 104 cells/ml tissue culture medium. Under these conditions growth coefficients were 3-fold greater in microcarriers when compared to conventional monolayer culture in roux culture flasks. Maximum yield of virus antigen was achieved after 8 days culture.
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Hayle, A.J. Culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers. Archives of Virology 89, 81–88 (1986). https://doi.org/10.1007/BF01309881
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DOI: https://doi.org/10.1007/BF01309881