Summary
The DNA and polypeptide patterns of feline herpesvirus-1 (FHV-1), a virus usually associated with feline respiratory infections, were compared to those of five herpesvirus isolates from dogs. These canine isolates had been shown to be antigenically similar to FHV-1 by cross neutralization tests. DNA from FHV-1 (C-27 strain) and each canine isolate was digested with either Bam H I, Eco R 1 or Sal I and analyzed on 0.8 per cent agarose gels. The restriction digest patterns of the canine isolates were nearly identical to C-27 for all three restriction enzymes. Interestingly, all of the canine isolates showed a small extension of the largest Bam H I fragment (14.5 kb) that was not present in the C-27 strain. Bam H I digested FHV-1 DNA from clinical cases in cats had digest patterns that were very similar to the canine isolates and also showed an extension of the 14.5 kb fragment. Southern blotting experiments revealed that DNA from the canine isolates has extensive homology to C-27 DNA. SDS-PAGE analysis of radiolabeled polypeptides from C-27 and the canine isolates showed identical virion-associated polypeptide profiles. In addition, a goat anti-FHV-1 antiserum precipitated three glycoprotein antigens from the canine isolates with migration patterns that were identical to the three major antigenic glycoproteins found on C-27. Hind III and Eco R 1 digestion patterns of canine herpesvirus DNA showed no similarity to C-27 DNA. In addition, canine herpesvirus DNA had no homology to C-27 DNA under the stringent conditions used.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Carmichael, L. E.: Herpes canis: Aspects of pathogenesis and immune response. J. Amr. Vet. Med. Assoc.156, 1714–1721 (1970).
Eberle, R., Courtney, R.: Preparation and characterization of specific antisera to individual glycoprotein antigens comprising the major glycoprotein region of herpes simplex type 1. J. Virol.35, 982–987 (1980).
Evermann, J. F., Bryan, G. M., McKeirnan, A. J.: Isolation of a calicivirus from a case of canine glossitis. Canine Pract.8, 36–39 (1981).
Evermann, J. F., McKeirnan, A. J., Ott, R. L., Reed, L. A.: Diarrheal condition in dogs associated with viruses antigenically related to feline herpesvirus. Cornell Vet.72, 285–291 (1982).
Gustafson, D. P.: Pseudorabies. In:Leman, A. D., Glock, R. D., Mengeling, W. L., Penny, R. H. C., Scholl, E., Straw, B. (eds.), Diseases of Swine, 209–223. Ames, Iowa: Iowa State University Press 1981.
Hermann, S. C., Gaskell, R. M., Ehlers, B., Ludwig, H.: Characterization of the feline herpesvirus genome and molecular epidemiology of isolates from natural outbreaks and latent infections. In:Wittman, G., Gaskell, R. M., Rziha, H. J. (eds.), Latent Virus Infections in Veterinary Medicine, 321–336. Boston, Mass.: Nijhoff 1984.
Horvath, Z. A., Bartha, L., Papp, L., Juhasz, M.: On feline rhinotracheitis. Acta Vet.15, 415–420 (1965).
Johnson, R. H., Spadbrow, P. B.: Isolation from dogs with severe enteritis of a parvovirus related to feline panleucopaenia virus, letter. Aust. Vet. J.55, 151 (1979).
Karpas, A., King, N. W., Garcia, F. G., Calva, F., Cross, R. E.: Canine tracheobronchitis: Isolation and characterization of the agent with experimental production of the disease. Proc. Soc. Expt. Biol. Med.127, 45–52 (1982).
Kessler, S. W.: Cell membrane antigen isolation with the staphylococcal protein-A antibody adsorbent. J. Immunol.117, 1482–1490 (1978).
Lemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London)227, 680–685 (1970).
Laskey, R. A., Mills, A. D.: Quantitative film detection of3H and14C in polyacrylamide gels by fluorography. Eur. J. Biochem.56, 335–341 (1975).
Maniatis, T., Fritsch, E., Sambrook, J.: Molecular Cloning: A Laboratory Manual, 109–112. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1982.
Maes, R. K., Fritsch, S. L., Herr, L. L., Rota, P. A.: Immunogenic proteins of feline rhinotracheitis virus. J. Virol.51, 259–262 (1984).
Pereira, L., Cassai, R., Honess, R., Roizman, B., Temi, M., Nahmias, A.: Variability in the structural polypeptides of herpes simplex virus 1 strains: Potential application in molecular epidemiology. Infect. Immun.13, 271–275 (1976).
Poste, G., King, N.: Isolation of a herpesvirus from the canine gential tract: Association with infertility, stillbirth, and abortions. Vet. Rec.88, 229–233 (1971).
Povery, R. C.: A review of felinerhinotracheitis virus. Comp. Immun. Microbiol. Infect. Dis.2, 373–387 (1979).
Roizman, B.: The family herpesviridae: general description, taxonomy, and classification. In:Roizman, B. (ed.), The Herpesviruses, Vol. 1, 1–23. New York: Plenum 1981.
Rota, P., Maes, R. K., Ruyechan, W.: Physical characterization of the genome of feline herpesvirus-1. Abstracts of the Ninth International Herpesvirus Workshop, Seattle, Wash., 124. 1984.
Sormiento, M., Hoffey, M., Spear, P.: Membrane proteins specified by herpes simplex virus III: Role of glycoprotein VP7 (B2) in virion infectivity. J. Virol.29, 1149–1158 (1979).
Southern, E.: Detection of specific fragments among DNA fragments separated by gel electrophoresis. J. Mol. Biol.98, 503–517 (1975).
Author information
Authors and Affiliations
Additional information
With 5 Figures
Rights and permissions
About this article
Cite this article
Rota, P.A., Maes, R.K. & Evermann, J.F. Biochemical and antigenic characterization of feline herpesvirus-1-like isolates from dogs. Archives of Virology 89, 57–68 (1986). https://doi.org/10.1007/BF01309879
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309879