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A comparison of the biological properties of type 2 plaque-producing agent derived from the Cal-1 strain of Marek's disease virus with other related viruses

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Summary

The serological and biological properties of the type 2 plaque-producing agent (PPA) derived from the Cal-1 strain of Marek's disease virus (MDV) were compared with those of reference strains of the three serotypes of MDV and herpesvirus of turkeys (HVT) groups; namely JM, HPRS-24 strains of MDV and the FC-126 strain of HVT for serotype 1, 2, and 3, respectively. By agar gel precipitation (AGP), indirect fluorescent antibody and virus neutralization tests, type 2 PPA was related but not identical to the FC-126 strain. By the AGP test, type 2 PPA showed a poor ability to synthesize B antigen and the A antigen was different from that of strain FC-126. To compare the virological characteristics of type 2 PPA with the reference strains, the release of cell-free virus into supernatants of infected cell cultures and titers of cell-free virus extracted sonically from infected cell cultures were examined. Cell-free type 2 PPA virus was easily detected in the supernatants and extracted from infected cell cultures. These properties were similar to reference strains of serotype 2 and 3. Next, the structural similarities of viral DNAs of type 2 PPA and strain FC-126 were examined by Southern blot hybridization. The restriction endonuclease-cleavage patterns of DNA of type 2 PPA were very similar but not identical to those of the FC-126 strain. In chickens inoculated with type 2 PPA, splenic lymphocytes supported a non-productive latent infection as did also those from chickens inoculated with the FC-126 or HPRS-24 strains. From these results, type 2 PPA appears to belong to serotype 3 of MDV and HVT groups. The origin of type 2 PPA is discussed.

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Authors and Affiliations

  1. Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, College of Dairying, Ebetsu, Hokkaido

    R. Kirisawa & Y. Kawakami

  2. Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara

    K. Hirai

  3. Aburahi Laboratories, Shionogi Research Laboratories, Shionogi & Co., Ltd., Koka-cho, Shiga

    S. Yachida

  4. Department of Pathology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka

    S. Kato

  5. Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan

    T. Mikami

Authors
  1. R. Kirisawa
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  2. K. Hirai
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  3. S. Yachida
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  4. Y. Kawakami
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  5. S. Kato
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  6. T. Mikami
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Kirisawa, R., Hirai, K., Yachida, S. et al. A comparison of the biological properties of type 2 plaque-producing agent derived from the Cal-1 strain of Marek's disease virus with other related viruses. Archives of Virology 89, 29–43 (1986). https://doi.org/10.1007/BF01309877

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  • DOI: https://doi.org/10.1007/BF01309877

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