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Identification and nucleotide sequence of a gene in feline herpesvirus type 1 homologous to the herpes simplex virus gene encoding the glycoprotein H

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Summary

A gene encoding the glycoprotein H (gH) homologue of feline herpesvirus type 1 was identified and sequenced. It was located immediately downstream of the thymidine kinase gene within anEco RI 6.6 kbp fragment. In addition, a partial UL 21 homologous gene was located downstream of the gH homologous gene. The primary translation product of the gH homologous gene is predicted to consist of 821 amino acids with a molecular weight of 92.5 kDa. It possesses several characteristics typical of transmembrane glycoproteins, including aN-terminal hydrophobic signal sequence,C-terminal transmembrane domain, and putativeN-linked glycosylation sites. Analysis of this protein revealed amino acid sequence homologies of 33.1% with equine herpesvirus type 1 (EHV-1) gH, 32.6% with EHV-4 gH, 29.1% with varicella-zoster virus gIII, 28.5% with pseudorabies virus gH, and 25.1% with herpes simplex virus type 1 gH. By Northern blot analysis, one of the transcripts specific for the gH homologous gene might be a mRNA of approximately 3.0 kb.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Japan

    K. Maeda, Y. Kawaguchi, N. Kamiya, M. Ono, Y. Tohya, C. Kai & T. Mikami

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  1. K. Maeda
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  2. Y. Kawaguchi
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  3. N. Kamiya
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  4. M. Ono
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  5. Y. Tohya
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  6. C. Kai
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  7. T. Mikami
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Maeda, K., Kawaguchi, Y., Kamiya, N. et al. Identification and nucleotide sequence of a gene in feline herpesvirus type 1 homologous to the herpes simplex virus gene encoding the glycoprotein H. Archives of Virology 132, 183–191 (1993). https://doi.org/10.1007/BF01309852

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  • DOI: https://doi.org/10.1007/BF01309852

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