Summary
The cRNA:vRNA hybridization technique was used to analyse H3N2 influenza virus isolates obtained from influenza patients in the United Kingdom and the U.S.A. (Alaska) during the epidemic season of 1982–1983. The majority of isolates differed from reference H3N2 influenza virus strains A/Bangkok/1/79 and A/Philippines/2/82 as well as from one another in the homology of nearly all the genes. No identical strains were detected among the isolates including the ones isolated in the same town and at the same time.
Similar content being viewed by others
References
Bonner, W. M., Laskey, R. H.: A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem.46, 83–88 (1974).
Burnet, F., Bull, D.: Changes in influenza virus associated with adaptation to passage in chick embryos. Austr. J. exp. Biol. Med. Sci.21, 55–69 (1943).
Cox, N. J., Bai, Z. S., Kendal, A. P.: Laboratory based surveillance of influenza A (H1N1) and influenza A (H3N2) viruses in 1980–1981. Antigenic and genomic analysis. Bull. W.H.O.61, 143–149 (1983).
Gaillard, R. K., Joklik, W. K.: Quantitation of the relatedness of reovirus serotypes 1, 2 and 3 at the gene level. Virology123, 152–164 (1982).
Ghendon, Y., Klimov, A., Blagoveshenskaya, O., Genkina, D.: Investigation of recombinants of human influenza and fowl plaque viruses. J. gen. Virol.43, 183–191 (1979).
Ghendon, Y., Klimov, A., Gorodkova, N., Döhner, L.: Genome analysis of influenza A virus strains isolated during an epidemic of 1979–1980. J. gen. Virol.56, 303–313 (1981).
Hay, A. J., Lomniczi, B., Bellamy, A. R., Shehel, J. J.: Transcription of influenza virus genome. Virology83, 337–355 (1977).
Hugentobler, A. L., Schild, G. C., Oxford, J. S.: Differences in the electrophoretic migration rates of polypeptides and RNAs of recent isolates of influenza B viruses. Arch. Virol.69, 197–207 (1981).
Ito, J., Joklik, W. K.: Temperature-sensitive mutants of reovirus. I. Patterns of gene expression by mutants of groups C, D and E. Virology50, 189–201 (1972).
Klimov, A. I., Tikhonova, A. M., Gorodkova, N. V., Herrman, B., Döhner, L., Ghendon, Y. Z.: Genome analysis of influenza virus strains isolated in 1979–1983. Acta Virol.26, 479–486 (1984).
Krystal, M., Buonagurio, D., Young, J. F., Palese, P.: Sequential mutations in the NS genes of influenza virus field strains. J. Virol.45, 547–554 (1983).
Nakajima, S., Cox, N. J., Kendal, A. P.: Antigenic and genomic analysis of influenza A (H1N1) viruses from different regions of the world, February 1978 to March 1980. Infect. Immun.32, 287–294 (1981).
Oxford, J. S., Corcoran, T., Schild, G. C.: Intratypic electrophoretic heterogenicity of structural and nonstructural polypeptides of human influenza A viruses. J. gen. Virol.56, 431–436 (1981).
Oxford, J. S., Abbo, H., Corcoran, T., Webster, R. G., Smith, A. J., Grill, E. A., Schild, G. C.: Antigenic and biochemical analysis of field isolates of influenza virus B: evidences for intra- and inter-epidemic variations. J. gen. Virol.64, 2367–2377 (1983).
Palese, P., Young, J.: Variation of influenza A, B and C viruses. Science215, 1468–1474 (1982).
Pereira, M. S., Chakraverty, P., Cunningham, P., Webster, R. G.: The use of monoclonal antibodies for the antigenic analysis of influenza A viruses. Bull. W.H.O.63 (1985).
Schild, G. C., Oxford, J. S., de Jong, J. C., Webster, R. G.: Evidence for host-cell selection of influenza virus antigenic variants. Nature303, 706–709 (1983).
Webster, R. G., Laver, W. G., Air, G. M., Schild, G. C.: Molecular mechanisms of variation in influenza viruses. Nature296, 115–121 (1982).
Author information
Authors and Affiliations
Additional information
With 3 Figures
Rights and permissions
About this article
Cite this article
Klimov, A.I., Ghendon, Y.Z. Analysis of the genome of influenza A virus strains (H3N2) isolated during the epidemic season of 1982–1983. Archives of Virology 86, 253–262 (1985). https://doi.org/10.1007/BF01309829
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309829