Summary
Lactic dehydrogenase virus (LDV) infected cells were localised in cryostat sections of infected adult and suckling mice by fluorescent antibody staining. In almost every organ except brain and spinal cord, LDV infected cells were present in interstitial connective tissue, including dermis and submucosa of gastrointestinal and urinogenital tracts. They were also present in liver sinusoids and red pulp of spleen. The tissue distribution, shape, and fluorescence staining pattern of infected cells were similar in adult and suckling mice. The reactivity with monoclonal antibody to mouse macrophages (F4/80) and to Ia antigens indicated that infected cells were Ia antigen positive macrophages, and this was confirmed in double labelling experiments. Peak numbers of LDV infected cells were seen in every organ 12–18 hours post infection (p.i.), disappearing rapidly thereafter until at 48 hours p.i. they could no longer be detected. At the same time Ia positive cells fell to undetectable levels, although macrophages were still present in reduced numbers. At 7 days p.i. the total number of macrophages in sections had returned to normal, but the number of Ia positive cells remained at low levels.
Macrophages recovered from peritoneal cavity and spleen of intraperitoneally infected mice were also studied. Ia positive cells had virtually disappeared from peritoneal cavity at 24–48 hours, and from spleen at 24–72 hours. Three weeks after infection the proportion of Ia positive cells was still low when compared with that of normal mice suggesting selective loss of these cells.
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Austyn, J. M., Gordon, S.: F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol.11, 805–815 (1981).
Crispens, C. G., Jr.: Studies on the response of SJL/J mice to infection with the lactate dehydrogenase virus. Arch. ges. Virusforsch.35, 177–182 (1971).
Frelinger, J. A., Niederhuber, J. E., David, C. S., Shreffler, D. C.: Evidence for the expression of Ia (H-2 associated) antigens on thymus derived lymphocytes. J. Exp. Med.140, 1273–1284 (1974).
Fukumoto, T., McMaster, W. R., Williams, A. F.: Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and Class I antigens in rat thymus. Eur. J. Immunol.12, 237–243 (1982).
Hammerling, G. J., Mauve, G., Goldberg, E., McDevitt, H. C.: Tissue distribution of Ia antigens: Ia on spermatozoa, macrophages and epidermal cells. Immunogenetics1, 428–437 (1975).
Hauser, S. L.: Immunohistochemical staining of human brain with monoclonal antibodies that identify lymphocytes, monocytes and the Ia antigen. J. Neuroimmunology5, 197–205 (1983).
Hart, D. N. J., Fabre, J. W.: Endogenously produced Ia antigens within cells of convoluted tubules of rat kidney. J. Immunol.126, 2109–2113 (1981).
Hart, D. N. J., Fabre, J. W.: Demonstration and characterisation of Ia-positive dendritic cells in the interstitial connective tissues of rat heart and other tissues, but not brain. J. Exp. Med.154, 347–361 (1981).
Inada, T., Mims, C. A.: Mouse Ia antigens are receptors for lactate dehydrogenase virus. Nature309, 59–61 (1984).
Klareskog, L., Tjernlund, U. M., Forsum, U., Peterson, P. A.: Epidermal Langerhans cells express Ia antigens. Nature268, 248–250 (1977).
Lu, C. T., Calamal, E. G., Unanue, E. R.: A defect in the antigen-presenting macrophages from neonatal mice. Nature282, 327–329 (1979).
Mayrhofer, G., Pugh, C. W., Barclay, A. N.: The distribution, ontogeny and origin in the rat of Ia positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine. Eur. J. Immunol.13, 112–182 (1983).
Notkins, A. L.: Lactic dehydrogenase virus. Bacteriol. Rev.29, 143–160 (1965).
Pierres, M., Kourilsky, F. M., Rebouah, J. P., Dosseto, M., Caillol, D.: Distinct epitopes on Ik gene products identified by monoclonal antibodies. Eur. J. Immunol.10, 950–957 (1980).
Porter, D. D., Porter, H. G., Deerhake, B. B.: Immunofluorescence assay for antigen and antibody in lactic dehydrogenase virus infection of mice. J. Immunol.102, 431–436 (1968).
Riley, V.: Persistence and other characteristics of the lactate dehydrogenase-elevating virus (LDH virus). Progr. Med. Virol.18, 198–213 (1974).
Rodgers, B. C., Mims, C. A.: Interaction of influenza virus with mouse macrophages. Infect. Immun.31, 751–757 (1981).
Rowden, G., Lewis, M. G., Sullivan, A. K.: Ia antigen expression on human epidermal Langerhans cells. Nature248, 247–248 (1977).
Rowson, K.E.K., Mahy, B.W.J.: Lactic Dehydrogenase Virus. (Virol. Monographs, Vol. 13). Wien-New York: Springer 1975.
Sachs, D. H., Cone, J. L.: A mouse “B” cell alloantigen determined by gene(s) linked to the major histocompatibility complex. J. Exp. Med.138, 1289–1304 (1973).
Steinman, R. M., Cohn, Z. A.: Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, Quantitation, tissue distribution. J. Exp. Med.137, 1142–1162 (1973).
Stueckmann, J. A., Ritzi, D. M., Holth, M., Smith, M. S., Swart, W. J., Cafruny, W. A., Plagemann, P. G. W.: Replication of lactate dehydrogenase-elevating virus in macrophages. I. Evidence for cytocidal replication. J. gen. Virol.59, 245–262 (1982).
Stueckmann, J. A., Holth, M., Swart, W. J., Kowalchyk, K., Smith, M. S., Wolstenholke, A. J., Cafruny, W. A., Plagemann, P. G. W.: Replication of lactate dehydrogenase-elevating virus in macrophages. II. Mechanism of persistent infection in mice and cell culture. J. gen. Virol.59, 263–272 (1982).
Ting, J. P. T., Shigekawa, B. L., Scottlinthicum, D., Weiner, O. P., Frelinger, J. A.: Expression and synthesis of murine immune response-associated (Ia) antigens by brain cells. Proc. Natl. Acad. Sci. U.S.A.78, 3170–3174 (1981).
Tong, S. L., Stueckmann, J., Plagemann, P. G. W.: Autoradiographic method for detection of lactate dehydrogenase-elevating virus-infected cells in primary mouse macrophage cultures. J. Virology22, 219–227 (1977).
Wekerle, H., Ketelsen, U. P.: Thymic nurse cells-Ia-bearing epithelium involved in T-lymphocyte differentiation? Nature283, 402–404 (1980).
Wroblewski, F., Landue, J. S.: Lactic dehydrogenase activity in blood. Proc. Exp. Soc. Biol. Med.90, 210–213 (1963).
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Inada, T., Mims, C.A. Pattern of infection and selective loss of Ia positive cells in suckling and adult mice inoculated with lactic dehydrogenase virus. Archives of Virology 86, 151–165 (1985). https://doi.org/10.1007/BF01309821
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DOI: https://doi.org/10.1007/BF01309821