Archives of Virology

, Volume 124, Issue 3–4, pp 321–332 | Cite as

Coxsackie B 1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness

  • K. Modalsli
  • G. Bukholm
  • S. O. Mikalsen
  • M. Degré
Original Papers

Summary

To analyze the possible mechanisms by which coxsackie B 1 virus infection affects the invasiveness ofShigella flexneri, we have studied the influence of intracellular levels of Na+ and K+, ATPase activity, cytoplasmic membrane potential, cAMP level and cell communication through gap junctions. 3 h after adsorption of viable or UV-inactivated coxsackie B 1 virus the Na+-K+ gradient of the cell collapsed, ATPase activity decreased, the cytoplasmic membranic potential-dependent tetraphosphonium ion uptake were reduced. No changes in cAMP or intercellular cell communication were observed.S. flexneri invasiveness in HEp-2 cell pretreated with viable or UV-inactivated coxsackie B 1 virus was enhanced, but bacterial invasiveness was unchanged in K+-depleted HEp-2 cells, cell cultures with high intracellular Na+ content or ouabain pre-treated cells compared to control cells. We found no correlation between the enhanced bacterial invasiveness in the early phase of coxsackie B 1 virus infection in HEp-2 cell cultures and intracellular K+ depletion, high intracellular Na+ content, inhibited Na+-K+ ATPase activity or membranic depolarization.

References

  1. 1.
    Degré M (1970) Synergistic effect in viral-bacterial infection. 2. Influence of viral infection on the phagocytic ability of alveolar macrophages. Acta Pathol Microbiol Scand [B] 78: 41–50Google Scholar
  2. 2.
    Jakab GJ, Warr GA, Sannes PL (1980) Alveolar macrophage ingestion and phagosome-lysosome fusion defect associated with virus pneumonia. Infect Immun 27: 960–968Google Scholar
  3. 3.
    Degré M (1971) Synergistic effect in viral-bacterial infection. 5. Functional studies on the role of the ciliary activity in the mouse trachea. Acta Pathol Microbiol Scand [B] 79: 137–141Google Scholar
  4. 4.
    Davison VV, Sandford BA (1981) Adherence ofStaphylococcus aureus to influenza A virus infected Madin-Darby canine cell culture. Infect Immun 32: 118–126Google Scholar
  5. 5.
    Bukholm G, Holberg-Petersen M, Degré M (1985) Invasiveness ofSalmonella typhimurium in HEp-2 cell cultures preinfected with UV-inactivated coxsackie B 1 virus. Acta Pathol Microbiol Immunol Scand [B] 92: 45–51Google Scholar
  6. 6.
    Bukholm G, Modalsli K, Degré M (1986) Effect of measles-virus infection and interferon treatment on invasiveness ofShigella flexneri in HEp-2 cell cultures. J Med Microbiol 22: 335–341Google Scholar
  7. 7.
    Bukholm G, Bjørnland K, Ellekjaer H, Berdal BP, Degré M (1988) Vesicular stomatitis virus infection enhances invasiveness ofSalmonella thyphimurium. Acta Pathol Microbiol Immunol Scand [B] 96: 400–406Google Scholar
  8. 8.
    Bukholm G (1989) Human rotavirus infection enhances invasiveness of enterobacteria in MA-104 cells. Acta Pathol Microbiol Immunol Scand [B] 96: 1118–1124Google Scholar
  9. 9.
    Modalsli K Bukholm G, Degré M (1990) Coxsackie B 1 virus infection enhances the bacterial invasiveness, the phagocytosis and the membrane permeability in HEp-2 cells. Acta Pathol Microbiol Immunol Scand [B] 98: 489–495Google Scholar
  10. 10.
    Schaefer A, Zibirre R, Kabus P, Kuhne J, Koch G (1982) Alterations in plasma-membrane functions after poliovirus infection. Biosci Rep 22: 613–615Google Scholar
  11. 11.
    Pasternak CA, Micklen KJ (1981) Virally induced alterations in cellular permeability: a basis of cellular physiological damage. Biosci Rep 1: 431–448Google Scholar
  12. 12.
    Foster KA, Gill KJ, Micklen KJ, Pasternack CA (1980) Survey of virally mediated permeability changes. Biochem J 190: 639–646Google Scholar
  13. 13.
    Rueckert RR, Pallansch MA (1981) Preparation and characterization of encephalo-myocarditis (EMC) virus. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 78. Academic Press, New York, pp 315–325Google Scholar
  14. 14.
    Rueckert RR (1985) Multiplication of picornavirus. In: Fields BN, Knipe DM (eds) Fundamental virology. Raven, New York, pp 358–383Google Scholar
  15. 15.
    Bukholm G, Johansen BV, Namork E, Lassen J (1982) Bacterial adhesiveness and invasivenesss in cell culture monolayer 1. A new light optical method evaluated by scanning electron microscopy. Acta Pathol Microbiol Immunol Scand [B] 90: 403–408Google Scholar
  16. 16.
    Madshus IH, Sandvig K, Olsnes S, van Deurs B (1987) Effect of reduced endocytosis by hypotonic shock and potassium depletion of the infection of HEp-2 cells by picornaviruses. J Cell Physiol 131: 14–22Google Scholar
  17. 17.
    Barret A (1972) Lysosomal enzymes. In: Dingle JT (ed) Lysosomes, a laboratory hand book. North-Holland, Amsterdam, pp 46–135Google Scholar
  18. 18.
    Schaefer A, Kuhne J, Zibirre R, Koch G (1982) Poliovirus induced alterations in HeLa cell membrane functions. J Virol 44: 444–449Google Scholar
  19. 19.
    Nair CN (1981) Monovalent cation metabolism and cytopathic effects of poliovirus-infected HeLa cells. J Virol 37: 268–273Google Scholar
  20. 20.
    Carrasco L (1981) Modification of membrane permeability induced by animal viruses early in infection. Virology 113: 623–629Google Scholar
  21. 21.
    Mahoney EM, Scott WA, Landsberger FR, Hamill AL, Cohn ZA (1980) Influence of fatty acyl substitution on the composition and function of macrophage membranes. J Biol Chem 225: 4910–4917Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • K. Modalsli
    • 1
  • G. Bukholm
    • 1
  • S. O. Mikalsen
    • 2
  • M. Degré
    • 1
  1. 1.Kaptein W. Wilhelmsen og Frues Bakteriologiske InstituttUniversity of OsloOsloNorway
  2. 2.Laboratory for Environmental and Occupational Cancer, Institute for Cancer ResearchThe Norwegian Radium HospitalOsloNorway

Personalised recommendations