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Replication of Influenza B virus in chicken embryos is suppressed by exogenous aprotinin

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Summary

Chicken embryo proteinases, one of which is a blood clotting factor Xa-like proteinase, are known to effectively cleave the haemagglutinin (HA) of Influenza B viruses to permit their replication in chicken embryonated eggs. Here we show that injection of the serine proteinase inhibitor, aprotinin, into the allantoic cavity of eggs infected with Influenza B/Hong Kong/73 and B/Lee/40 viruses suppresses the viral HA cleavage and reduces the virus proteolytic activation and replication. Effective inhibition dose was determined as ∼10.0 µg of aprotinin per embryo that corresponds to 0.1 µM concentration. However, heparin, which is known to be a direct inhibitor of the Factor Xa, was not able to suppress Influenza B virus hemagglutinin cleavage and replication in chicken embryo system. These data shed light on the pattern of proteinases involved in the Influenza B virus proteolytic activation and indicate that aprotinin possesses antiviral potential against Influenza B viruses.

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Authors and Affiliations

  1. The D.I. Ivanovsky Virology Institute, Moscow, Russia

    O. P. Zhirnov, P. B. Golyando & A. V. Ovcharenko

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  1. O. P. Zhirnov
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  2. P. B. Golyando
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  3. A. V. Ovcharenko
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Zhirnov, O.P., Golyando, P.B. & Ovcharenko, A.V. Replication of Influenza B virus in chicken embryos is suppressed by exogenous aprotinin. Archives of Virology 135, 209–216 (1994). https://doi.org/10.1007/BF01309780

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  • DOI: https://doi.org/10.1007/BF01309780

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