Summary
The BamHI-BamHI fragment of the env gene of bovine leukemia virus (BLV) cloned in pMMEx expression vector was transfected into Chinese hamster cells. Monoclonal antibodies (MAbs) directed against both conformational and sequential epitopes of gp51 of BLV recognized viral polypeptides expressed in hamster cells in Western blotting and enzyme-linked immunosorbent assay.
This is a preview of subscription content, log in via an institution to check access.
References
Bruck C, Renconnet N, Portetelle D, Cleuter Y, Mammerickx M, Burny A, Mamoun R, Guillemain B, Van der Maaten M, Ghysdael J (1984) Biologically active epitopes of bovine leukemia virus glycoprotein gp51: their dependence on protein glycosylation and genetic variability. Virology 136:20–31
Ford DK, Yerganian G (1958) Observations on the chromosomes of Chinese hamster cells in tissue culture. J Natl Cancer Inst 21: 393–425
Lee F, Mulligan R, Berg P, Ringold G (1981) Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumors virus chimaeric plasmids. Nature 294:228–232
Mamoun R, Astier T, Guillemain B, Duplan JF (1983) Bovine lymphosarcoma: processing of bovine leukemia virus-coded proteins. J Gen Virol 64:2791–2795
Portetelle D, Couez D, Bruck C, Kettmann R, Mammerickx M, Van der Maaten M, Brasseur R, Burny A (1989) Antigenic variants of bovine leukemia virus (BLV) are defined by amino acid substitutions in the NH2 part of the envelope glycoprotein gp51. Virology 169:27–33
Seigneurin D, Guillaud PH (1991) Ki-67 antigen, a cell cycle and tumor growth marker (in French). Pathol Biol (Paris) 39:1020–1028
Slavikova K, Kettmann R, Reinerova M, Zajac V, Portetelle D, Burny A (1987) Provirus, integration of bovine leukemia virus into DNA of infected human myeloma cells. Neoplasma 34:653–657
Slavikova K, Zajac V, Klobusicka M, Aida Y, Stevurkova V (1993) gp51 of bovine leukemia virus gene expression in hamster cells. Int J Cancer 53:640–644
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Tsuchida N, Uesugi S (1980) Structure and functions of the Kirsten murine sarcoma virus genome: molecular cloning of biologically active Kirsten murine sarcoma virus DNA. J Virol 38:720–727
Zajac V, Slavikova K (1989) Expression of a bovine leukaemia virus envelope fusion protein inE. coli. Folia Biol 35:35–41
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zajac, V., Slavikova, K. & Babusikova, O. Expression of env gene of bovine leukemia virus in rodent cells. Archives of Virology 135, 201–207 (1994). https://doi.org/10.1007/BF01309779
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309779