Summary
To investigate the role of Hantaan virus envelope glycoprotein in infection, a panel of monoclonal antibodies (MAbs) was examined in vitro with several serological tests and in vivo by passive transfer experiments in mice. An antigenic site, specific for the inhibition of infected cell focus was detected with the focus inhibition neutralization test (FINT), in addition to the neutralization related antigenic sites, which were revealed by the ordinary focus reduction neutralization test (FRNT). Suckling mice were given the MAbs by passive transfer followed by lethal Hantaan virus challenge. All neutralizing MAbs detected by either FRNT or FINT protected all mice from lethal infection, confirming the importance of the antigenic sites as a protective antigen. Mice given non-neutralizing MAbs by passive transfer, however, began to die earlier than the control group; mean time to death (18.2±2.1 to 21.5±2.8 days) being significantly shorter than that of the control group (25.8±1.8, p<0.01, Mann-Whitney,U probability test). Virus titers in brains of mice which died early, were about 10 times higher than those of control mice. These results indicated the early death phenomenon of mice which was mediated by the antivirus antibody.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Arikawa J, Schmaljohn AL, Dalrymple JM, Schmaljohn CS (1989) Characterization of Hantaan virus envelope glycoprotein antigenic determinants defined by monoclonal antibodies. J Gen Virol 70: 615–624
Arikawa J, Takashima I, Hashimoto N (1985) Cell fusion by haemorrhagic fever with renal syndrome (HFRS) viruses and its application for titration of virus infectivity and neutralization antibody. Arch Virol 86: 303–313
Arikawa J, Takashima I, Hashimoto N, Takahashi K, Yagi K, Hattori K (1986) Epidemiological studies of hemorrhagic fever with renal syndrome (HFRS) related virus infection among urban rats in Hokkaido, Japan. Arch Virol 88: 231–240
Fanko MC, Gibbs CJ Jr, Lee PW, Gajdusek DC (1983) Monoclonal antibodies specific for hantaan virus. Proc Natl Acad Sci USA 80: 4149–4153
Gould EA, Buckley A (1989) Antibody-dependent enhancement of yellow fever and Japanese encephalitis virus neurovirulence. J Gen Virol 70: 1605–1608
Gould EA, Buckley A, Groeger BK, Cane PA, Doenhoff M (1987) Immune enhancement of yellow fever virus neurovirulence for mice: studies of mechanisms involved. J Gen Virol 68: 3105–3112
Grosfeld H, Velan B, Leitner M, Cohen S, Lustig S, Lachmi BE, Shafferman A (1989) Semliki forest virus E2 envelope epitopes induce a non-neutralizing humoral response which protects mice against lethal challenge. J Virol 63: 3416–3422
Jiang YT (1983) A preliminary report on hemorrhagic fever with renal syndrome in China. Chinese Med J 96: 265–268
Kim GR, McKee Jr KT (1985) Pathogenesis of hantaan virus infection in suckling mice: clinical, virologic and serologic observations. Am J Trop Med Hyg 34: 388–395
Lähdevirta J, Savola J, Brummer-Korvenkontio M, Berndt R, Illikainen R, Vaheri A (1984) Clinical and serological diagnosis of Nephropathia epidemica, the mild type of hemorrhagic fever with renal syndrome. J Infect Dis 9: 230–238
Lee HW, Lee PW, Baek LJ, Chu YK (1989) Seroepidemiological features of haemorrhagic fever with renal syndrome in the Republic of Korea from 1980 to 1987. JE HFRS Bull 3: 45–48
Lee HW, Lee PW, Baek LJ, Chu YK (1990) Geographical distribution of hemorrhagic fever with renal syndrome and hantaviruses. Arch Virol [Suppl] 1: 5–18
Lee HW, Lee PW, Johnson KM (1978) Isolation of the etiological agent, Korean hemorrhagic fever. J Infect Dis 137: 298–308
McLain L, Dimmock NJ (1989) Protection of mice from lethal influenza by adoptive transfer of non-neutralizing haemagglutination-inhibiting IgG obtained from the lungs of infected animals treated with defective interfering virus. J Gen Virol 70: 2615–2624
Myasnikov YuA, Rezapkin GV, Shuikova ZV, Tkachenko EA, Ivanova AA, Nurgaleeva RG, Stepanenko AG, Vereshchagin NN, Loginov AI, Bagan RN, Zaitseva AA, Levacheva ZA, Bobylkova TV, Ishcheryakova AM, Boruta VV (1984) Antibodies to the HFRS virus in the human population of European RSFSR as detected by radioimmunoassay. Arch Virol 79: 109–115
Nagai T, Tanishita O, Takahashi Y, Yamanouchi T, Domae K, Kondo K, Dantas Jr JR, Takahashi M, Yamanishi K (1985) Isolation of hemorrhagic fever with renal syndrome virus from leukocyte of rats and virus replication in cultures of rat and human macrophages. J Gen Virol 66: 1271–1278
Nakamura T, Yanagihara R, Gibbs JC Jr, Amyx LH, Gajdusek CD (1985) Different susceptibility and resistance of immunocompetent and immunodeficient mice to fatal hantaan virus infection. Arch Virol 86: 109–120
Nakamura T, Yanagihara R, Gibbs CJ Jr, Gajdusek CD (1985) Immune spleen cell-mediated protection against fatal hantaan virus infection in infant mice. J Infect Dis 151: 691–697
Prabhakar BS, Nathanson N (1981) Acute rabies death mediated by antibody. Nature 290: 590–591
Putnak RJ, Schlesinger JJ (1990) Protection of mice against yellow fever virus encephalitis by immunization with a vaccinia virus recombinant encoding the yellow fever virus non-structural proteins, NS1, NS2a and NS2b. J Gen Virol 71: 1697–1702
Schlesinger JJ, Brandriss MW, Putnak JR, Walsh EE (1990) Cell surface expression of yellow fever virus non-structural glycoprotein NS1: consequences of interaction with antibody. J Gen Virol 71: 593–599
Schlesinger JJ, Brandriss MW, Walsh EE (1987) Protection of mice against Dengue 2 virus encephalitis by immunization with the dengue 2 virus nonstructural glycoprotein NS1. J Gen Virol 68: 853–857
Schmaljohn CS, Chu YK, Schmaljohn AL, Dalrymple JM (1990) Antigenic subunits of Hantaan virus expressed by baculovirus and vaccinia virus recombinants. J Virol 64: 3162–3170
Schmaljohn CS, Dalrymple JM (1983) Analysis of Hantaan virus RNA: evidence for a new genus of Bunyaviridae. Virology 131: 482–491
Schmaljohn CS, Hasty SE, Harrison SA, Dalrymple JM (1983) Characterization of Hantaan virions, the prototype virus of hemorrhagic fever with renal syndrome. J Infect Dis 148: 1005–1012
Schmaljohn AL, Johnson ED, Dalrymple JM, Cole GA (1982) Non-neutralizing monoclonal antibodies can prevent lethal alphavirus encephalitis. Nature 297: 70–72
Song G, Hang CS, Liao HX, Fu JL, Gao GZ, Qiu HL, Zhang QF (1984) Antigenic difference between viral strains causing classical and mild types of epidemic hemorrhagic fever with renal syndrome in China. J Infect Dis 150: 889–894
Tamura M, Asada H, Kondo K, Tanishita O, Kurata T, Yamanishi K (1989) Pathogenesis of hantaan virus in mice. J Gen Virol 70: 2897–2906
Tan CHc, Yap EH, Singh M, Deubel V, Chan YC (1990) Passive protection studies in mice with monoclonal antibodies directed against the nonstructural protein NS3 of dengue 1 virus. J Gen Virol 71: 745–748
Tanishita O, Takahashi Y, Okuno Y, Yamanishi K, Takahashi M (1984) Evaluation of focus reduction neutralization test with peroxidase-antiperoxidase staining technique for hemorrhagic fever with renal syndrome virus. J Clin Microbiol 20: 1213–1215
Umino Y, Kohama T, Sato A, Sugiura A (1990) Protective effect of monoclonal antibodies to Newcastle disease virus in passive immunization. J Gen Virol 71: 1199–1203
Webb HE, Wight DGD, Platt GS, Smith CEG (1968) Langat virus encephalitis in mice. I. The effect of the administration of specific antiserum. J Hyg 66: 343–354
Weiss RC, Scott FW (1981) Antibody-mediated enhancement of disease in feline infectious peritonitis: comparisons with dengue hemorrhagic fever. Comp Immun Microbiol Infect Dis 4: 175–189
World Health Organization (1983) Hemorrhagic fever with renal syndrome: memorandum from a WHO meeting. Bull WHO 61: 269–275
Yanagihara R, Gajdusek CD (1987) Hemorrhagic fever with renal syndrome: Global epidemiology and ecology of hantavirus infections. Med Virol 6: 198–202
Yao JS, Kariwa H, Takashima I, Arikawa J, Hashimoto N (1991) Antibody-dependent enhancement of hantavirus infection in macrophage cell lines. Arch Virol 122: 107–118
Zhang XK, Takashima I, Mori F, Hashimoto N (1989) Comparison of virulence between two strains ofRattus serotype hemorrhagic fever with renal syndrome (HFRS) virus in newborn rats. Microbiol Immunol 33: 195–205
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Arikawa, J., Yao, J.S., Yoshimatsu, K. et al. Protective role of antigenic sites on the envelope protein of Hantaan virus defined by monoclonal antibodies. Archives of Virology 126, 271–281 (1992). https://doi.org/10.1007/BF01309700
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309700