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Phagocytosis reduces HIV-1 production in human monocytes/macrophages infected in vitro

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Summary

The addition of ingestible particles (opsonized erythrocytes or latex beads) or a phorbol ester activates monocytes — derived human macrophages (MDHM) cultured in vitro, and markedly reduces virion release from HIV-infected MDHM as well as their ability to transmit the infection to cocultured lymphoid CD 4-positive CEM cells.

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References

  1. von Briesen H, Andreesen R, Rübsamen-Waigmann H (1990) Systemic classification of HIV biological subtypes on lymphocytes and monocytes/macrophages. Virology 178: 597–602

    Google Scholar 

  2. Brunner D, Pedersen NC (1989) Infection of peritoneal macrophages in vitro and in vivo with feline immunodeficiency virus. J Virol 63: 5483–5488

    Google Scholar 

  3. Di Rienzo AM, Petronini PG, Guétard D, Favilla R, Borghetti AF, Montagnier L, Piedimonte G (1992) Modulation of cell growth and host protein synthesis during HIV infection in vitro. J AIDS 5: 921–929

    Google Scholar 

  4. Greene WC (1990) Regulation of HIV-1 gene expression. Annu Rev Immunol 8: 453–475

    Google Scholar 

  5. Griffin GE, Leung K, Folks TM, Kunkel S, Nabel GJ (1989) Activation of HIV gene expression during monocyte differentiation by induction of NF-kB. Nature 339: 70–73

    Google Scholar 

  6. Hammer SM, Gillis JM, Groopman JE, Rose RM (1986) In vitro modification of human immunodeficiency virus infection by granulocyte-macrophage colony stimulating factor and interferon. Proc Natl Acad Sci USA 83: 8734–8738

    Google Scholar 

  7. Koyanagi Y, O'Brien WA, Zhao JQ, Golde DW, Gasson JC, Chen ISY (1988) Cytokines alter production of HIV-1 from primary mononuclear phagocytes. Science 241: 1673–1675

    Google Scholar 

  8. Meltzer MS, Skillman DR, Gomatos PJ, Kalter DC, Gendelman HE (1990) Role of mononuclear phagocytes in the pathogenesis of human immunodeficiency virus infection. Annu Rev Immunol 8: 169–194

    Google Scholar 

  9. Meltzer MS, Skillman DR, Hoover DL, Hanson BD, Turpin JA, Kalter DC, Gendelman HE (1990) Macrophages and the human immunodeficiency virus. Immunol Today 11: 217–223

    Google Scholar 

  10. Narayan O, Clemens JE (1989) Biology and pathogenesis of lentivirus. J Gen Virol 70: 1617–1639

    Google Scholar 

  11. Narayan O, Kennedy-Stoskopf S, Sheffer D, Griffin DE, Cork LC (1983) Slow virus replication: the role of macrophages in the persistence and expression of visna viruses of sheep and goats. Infect Immun 41: 67–73

    Google Scholar 

  12. Osborn L, Kunkel S, Nabel GJ (1989) Tumor necrosis factor α and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kB. Proc Natl Acad Sci USA 86: 2336–2340

    Google Scholar 

  13. Pick E (1986) Microassays for superoxide and hydrogen peroxide production and nitroblue tetrazolium reduction using an enzyme immunoassay microplate reader. Methods Enzymol 132: 407–421

    Google Scholar 

  14. Piedimonte G, Petronini PG, Guétard D, Favier V, Borghetti AF, Montagnier L (1990) Protease activation during HIV infection in a CD 4-positive cell line. AIDS Res Hum Retroviruses 6: 251–259

    Google Scholar 

  15. Pomerantz RJ, Feinberg MB, Trono D, Baltimore D (1990) Lipopolysaccharide is a potent monocyte/macrophage-specific stimulator of human immunodeficiency virus type 1 expression. J Exp Med 172: 253–261

    Google Scholar 

  16. Stossel TP (1987) The molecular biology of phagocytes and the molecular basis of nonneoplastic phagocyte disorders. In: Stomatoyannopoulos G, Nienhuis AW, Leder P, Majerus PW (eds) The molecular bases of blood diseases. Saunders, Philadelphia, pp 499–533

    Google Scholar 

  17. Valentin A, Albert J, Fenyö EM, Åsiö B (1990) HIV-1 infection of normal human macrophage culture: implication for silent infection. Virology 177: 790–794

    Google Scholar 

  18. Wain-Hobson S, Vartanian JP, Henry M, Chenciner N, Cheynier R, Delassus S, Martins LP, Sala M, Nugeyre MT, Guétard D, Klatzmann D, Gluckman JC, Rozenbaum W, Barré-Sinoussi F, Montagnier L (1991) LAV revisited: origins of the early HIV-1 isolates from Institut Pasteur. Science 252: 961–965

    Google Scholar 

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Authors and Affiliations

  1. Institute of General Pathology, University of Parma, Parma

    G. Piedimonte, Lucia Silvotti, Anna Donatini & A. F. Borghetti

  2. Institute of Internal Medicine — Clinical Immunology, University of Ancona, Ancona

    Maria Montroni & G. Silvestri

  3. Institute of Biochemistry, University of Urbino, Urbino, Italy

    Luigia Rossi & M. Magnani

Authors
  1. G. Piedimonte
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  2. Maria Montroni
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  3. G. Silvestri
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  4. Lucia Silvotti
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  5. Anna Donatini
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  6. Luigia Rossi
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  7. A. F. Borghetti
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  8. M. Magnani
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Piedimonte, G., Montroni, M., Silvestri, G. et al. Phagocytosis reduces HIV-1 production in human monocytes/macrophages infected in vitro. Archives of Virology 130, 463–469 (1993). https://doi.org/10.1007/BF01309674

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  • DOI: https://doi.org/10.1007/BF01309674

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