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Host-virus interaction as defined by amplification of viral DNA and serology in lentivirus-infected sheep

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Summary

To correlate the presence of ovine lentivirus (OvLV) as detected by polymerase chain reaction (PCR) with detection of antibody, 42 sheep from a flock with enzootic OvLV infection were studied. The results of agar gel immunodiffusion (AGID), ELISA, and immunoblotting assays were compared, and leukocytes (blood, bone marrow, lymph node, and lung cells) were assessed for viral DNA by PCR usingpol and LTR primers; amplified products were detected by specific DNA and RNA probes. Based on the number of animals that had detectable viral DNA, the specificities of AGID, ELISA, and immunoblotting were 77%, 92%, and 95 or 100% (depending on which criterion was used to interpret immunoblot results), respectively. Only in animals with OvLV-associated disease was OvLV DNA detected in leukocyte DNA prior to the amplification of virus in culture and only in this group was high titer antibody detected to the OvLV major surface (gp 105) and transmembrane (gp 55) antigens. Animals that were both antibody and PCR-negative lacked histopathologic evidence of disease. From this study there was no indication that OvLV infection without the development of antibody occurs, and detection of OvLV DNA in animals with weak or partial serological reactions likely indicates early OvLV infection rather than false-positive PCR results.

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References

  1. Barban V, Querat G, Sauze N, Filippi P, Vigne R, Russo P, Vitu C (1984) Lentiviruses are naturally resident in a latent form in long-term ovine fibroblast cultures. J Virol 52: 680–682

    Google Scholar 

  2. Behyner DE, Riemann HP, Utterback W, Elmi CD, Franti CE (1991) Mass screening of cattle sera against 14 infectious disease agents, using an ELISA system for monitoring health in livestock. Am J Vet Res 52: 1699–1705

    Google Scholar 

  3. Bosgiraud C, Rucheton M, Coste J, Lemaire JM, Nicolas JA, Simeon de Buochberg M, Beaubatie L (1989) Detection of visna-maedi virus antibodies and antigens by immunoblotting. Ann Rech Vet 20: 187–193

    Google Scholar 

  4. Brodie SJ, Marcom KA, Pearson LD, Anderson BC, de la Concha-Bermejillo A, Ellis JA, DeMartini JC (1992) The effects of virus load in the pathogenesis of lentivirus-induced LIP. J Infect Dis 166: 531–541

    Google Scholar 

  5. Celum CL, Coombs RW, Lafferty W, Inui TS, Louie PH, Gates CA, McCreedy BJ, Egan R, Grove T, Alexander S, Koepsell T, Weiss N, Fisher L, Corey L, Holmes KK (1991) Indeterminate human immunodeficiency virus type 1 Western blots: Seroconversion risk, specificity of supplemental tests, and an algorithm for evaluation. J Infect Dis 164: 656–664

    Google Scholar 

  6. Chou NJ, Lee TH, Hatzakis A, Mandalaki T, McLane MF, Essex M (1988) Antibody responses in early human immunodeficiency virus type 1 infection in hemophiliacs. J Infect Dis 157: 805–811

    Google Scholar 

  7. Clements JE, Gdovin SL, Montelaro RC, Narayan O (1988) Antigenic variation in lentiviral diseases. Annu Rev Immunol 6: 139–159

    Google Scholar 

  8. Cutlip RC, Jackson TA, Laird GA (1977) Immunodiffusion test for ovine progressive pneumonia. Am J Vet Res 38: 1081–1084

    Google Scholar 

  9. Dawson M (1988) Lentivirus diseases of domesticated animals. J Comp Pathol 99: 401–419

    Google Scholar 

  10. Dodd RY, Fang CT (1990) The Western immunoblot procedure for HIV antibodies and its interpretation. Arch Pathol Lab Med 114: 240–245

    Google Scholar 

  11. Eisenstein BI (1990) The polymerase chain reaction. N Engl J Med 322: 178–183

    Google Scholar 

  12. Ellaurie M, Rubinstein A (1991) Correlation of serum antigen and antibody concentration with clinical features in HIV infection. Arch Dis Child 66: 200–203

    Google Scholar 

  13. Ellis JA, Lairmore MD, O'Toole DT, Campos M (1991) Differential induction of tumour necrosis factor alpha in ovine pulmonary macrophages following infection withCorynebacterium pseudotuberculosis, Pasteurella haemolytica, or lentiviruses. Infect Immun 59: 3254–3260

    Google Scholar 

  14. Ensoli F, Fiorelli V, Mezzaroma I, D'Offizi GP, Aiuti F (1990) Proviral sequences detection of human immunodeficiency virus in seronegative subjects by polymerase chain reaction. Mol Cell Probes 4: 153–161

    Google Scholar 

  15. Fahey JL, McKelvey EM (1965) Quantitative determination of serum immunoglobulins in antibody-agar plates. J Immunol 94: 84–90

    Google Scholar 

  16. Gendelman HE, Narayan O, Molineaux S, Clements JE, Ghotbi Z (1985) Slow persistent replication of lentiviruses: Role of tissue macrophages and macrophage-precursors in bone marrow. Proc Natl Acad Sci USA 82: 7086–7090

    Google Scholar 

  17. Haase AT (1986) Pathogenesis of lentivirus infections. Nature 322: 130–136

    Google Scholar 

  18. Hart C, Schochetman G, Spira T, Lifson A, Moore J, Galphin J (1988) Direct detection of HIV RNA expression in seronegative subjects. Lancet 2: 596–599

    Google Scholar 

  19. Ho DD, Pomerantz RJ, Kaplan JC (1987) Pathogenesis of infection with human immunodeficiency virus. N Engl J Med 317: 278–286

    Google Scholar 

  20. Horsburgh CR Jr, Ou CY, Jason J, Holmberg SD, Lifson AR, Moore JL, Ward JW, Seage GR, Mayer KH, Evatt BL, Schochetman G, Jaffe HW (1990) Duration of human immunodeficiency virus infection before detection of antibody. J Infect Dis 162: 542–545

    Google Scholar 

  21. Houwers DJ, Nauta IM (1989) Immunoblot analysis of the antibody response to ovine lentivirus infections. Vet Microbiol 19: 127–139

    Google Scholar 

  22. Imagawa DT, Lee MH, Wolinsky SM, Sano K, Morales F, Kwok S, Sninsky JJ, Nishanian PG, Georgi J, Fahey JL, Dudley J, Visscher BR, Deles R (1989) Human immunodeficiency virus type 1 infection in homosexual men who remain seronegative for prolonged periods. N Engl J Med 320: 1458–1462

    Google Scholar 

  23. Jackson JB, MacDonald KL, Cadwell J, Sullivan C, Kline WE, Hanson M, Sannerud KJ, Stramer SL, Fildes NJ, Kwok SY, Sninsky JJ, Bowman RJ, Polesky HF, Balfour HH, Osterholm MT (1990) Absence of HIV infection in blood donors with indeterminate Western blot tests for antibody to HIV-1. N Engl J Med 322: 217–222

    Google Scholar 

  24. Kajikawa O, Lairmore MD, DeMartini JC (1990) Analysis of antibody responses to phenotypically distinct lentiviruses. J Clin Microbiol 28: 764–770

    Google Scholar 

  25. Kitchin PA, Szotyori Z, Fromholc C, Almond N (1990) Avoidance of false positives. Nature 344: 210

    Google Scholar 

  26. Knowles D Jr, Cheevers W, McGuire T, Stem T, Gorham J (1990) Severity of arthritis is predicted by antibody response to gp 135 in chronic infection with caprine arthritisencephalitis virus. J Virol 64: 2396–2398

    Google Scholar 

  27. Kwok S, Higuchi R (1989) Avoiding false positives with PCR. Nature 339: 237–238

    Google Scholar 

  28. Lairmore MD, Rosadio RH, DeMartini JC (1986) Ovine lentivirus lymphoid interstitial pneumonia: rapid induction in neonatal lambs. Am J Pathol 125: 173–181

    Google Scholar 

  29. Lairmore MD, Akita GY, Russell HI, DeMartini JC (1987) Replication and cytopathic effects of ovine lentivirus strains in alveolar macrophages correlate with in vivo pathogenicity. J Virol 61: 4038–4042

    Google Scholar 

  30. Lange JMA, Paul DA, Huisman HG, De Wolf F, Van Den Berg H, Coutinho RA, Danner SA, Vander Noorda J, Goudsmit J (1986) Persistent HIV antigenemia and decline of HIV core antibodies associated with transition to AIDS. Br Med J 293: 1459–1462

    Google Scholar 

  31. Lantin JP, Graf I, Frei PC (1989) Serological diagnosis of HIV infection: incidence, outcome, and significance of partial reactions found in Western blot analysis. Clin Exp Immunol 76: 332–336

    Google Scholar 

  32. Leis J, Baltimore D, Bishop JM, Coffin J, Fleissner E, Goff SP, Oroszlan S, Robinson H, Skalka AM, Temin HM, Vogt V (1988) Standardized and simplified nomenclature for proteins common to all retroviruses. J Virol 62: 1808–1809

    Google Scholar 

  33. Loche M, Mach B (1988) Identification of HIV-infected seronegative individuals by direct diagnostic test based on hybridization to amplified viral DNA. Lancet 2: 418–421

    Google Scholar 

  34. Marcom KA, Pearson LD, Chung CS, Poulson JM, DeMartini JC (1991) Epitope analysis of capsid and matrix proteins of North American ovine lentivirus field isolates. J Clin Microbiol 29: 1472–1479

    Google Scholar 

  35. Meyerhans A, Cheynier R, Albert J (1989) Temporal fluctuations in HIV quasispecies in vivo are not reflected by sequential HIV isolates. Cell 58: 901–910

    Google Scholar 

  36. Molitor TW, Light MK, Schipper IA (1979) Elevated concentrations in serum immunoglobulins due to infection by ovine progressive pneumonia virus. Am J Vet Res 40: 69–72

    Google Scholar 

  37. Narayan O, Clements JE (1989) Biology and pathogenesis of lentiviruses. J Gen Virol 70: 1617–1639

    Google Scholar 

  38. Pedersen C, Nielsen CM, Vestergaard BF, Gerstoft J, Krogsgaard K, Nielsen JO (1987) Temporal relation of antigenemia and loss of antibodies to core antigens to development of clinical disease in HIV infection. Br Med J 295: 567–569

    Google Scholar 

  39. Petursson G, Hoff-Jorgensen R (1990) Developments in veterinary immunology: maedivisna and related diseases. Kluwer, Boston

    Google Scholar 

  40. Remington RD, Schork MA (1970) Statistics with applications to the biological and health sciences. Prentice-Hall, Englewood Cliffs

    Google Scholar 

  41. Schechter MT, Neumann PW, Weaver MS, Montaner JSG, Cassol SA, Le TN, Craib KJP, O'Shaughnessy MV (1991) Low HIV-1 proviral DNA burden detected by negative polymerase chain reaction in seropositive individuals correlates with slower disease progression. AIDS 5: 373–379

    Google Scholar 

  42. Scott GB (1991) HIV infection in children: clinical features and management. J Acquir Immune Defic Syndr 4: 109–115

    Google Scholar 

  43. Sheppard HW, Dondero D, Arnon J, Winkelstein W (1991) An evaluation of the polymerase chain reaction in HIV-1 seronegative men. J Acquir Immune Defic Syndr 4: 819–823

    Google Scholar 

  44. Simard CL, Briscoe MR (1990) An enzyme-linked immunosorbent assay for detection of antibodies to maedi-visna virus in sheep. II. Comparison to conventional agar gel immunodiffusion test. Can J Vet Res 54: 451–456

    Google Scholar 

  45. Simard CL, Briscoe MR (1990) An enzyme-linked immunosorbent assay for detection of antibodies to maedi-visna virus in sheep. I. A simple technique for production of antigen using sodium dodecyl sulfate treatment. Can J Vet Res 54: 446–450

    Google Scholar 

  46. Sonigo P, Alizon M, Staskus K, Klatzmann D, Cole S, Danos O, Retzel E, Tiollsais P, Haase A (1985) Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus. Cell 42: 369–382

    Google Scholar 

  47. Stoeckl E, Barrett N, Heinz FX, Banekovich M, Stingl G, Guggenberger K, Dorner F, Kunz C (1989) Efficiency of the polymerase chain reaction for the detection of human immunodeficiency virus type 1 DNA in the lymphocytes of infected persons: comparison to antigen-enzyme-linked immunosorbent assay and virus isolation. N Engl J Med 29: 249–255

    Google Scholar 

  48. Torfason EG, Gudnadottir M, Love A (1992) Comparison of immunoblots with neutralizing and complement fixing antibodies in experimental and natural cases of visnamaedi. Arch Virol 123: 47–58

    Google Scholar 

  49. Travis WD, Fox CH, Devaney KO, Weiss LM, O'Leary T, Ognibene FP, Suffredini AF, Rosen MJ, Cohen MB, Shelhamer J (1992) Lymphoid pneumonitis in 50 adult patients with the human immunodeficiency virus: lymphocytic interstitial pneumonitis versus non-specific interstitial pneumonitis. Hum Pathol 23: 529–541

    Google Scholar 

  50. Vollar A, Bidwell D (1986) Enzyme-linked immunosorbent assay. In: Rose NR, Friedman H, Fahey GL (eds) Manual of clinical laboratory immunology, 3rd edn. American Society for Microbiology, Washington, DC, p 108

    Google Scholar 

  51. Zanoni R, Pauli U, Peterhans E (1990) Caprine arthritis-encephalitis (CAE) and maedivisna viruses detected by the polymerase chain reaction (PCR). Experimentia 46: 316–319

    Google Scholar 

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Author notes

  1. S. J. Brodie

    Present address: Harvard Medical School, New England Regional Primate Center, Southborough, Massachusetts, USA

Authors and Affiliations

  1. Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado

    S. J. Brodie & J. C. DeMartini

  2. Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado

    S. J. Brodie & L. D. Pearson

  3. USDA-ARS, U.S. Sheep Experimental Station, Dubois, Idaho, USA

    G. D. Snowder

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  1. S. J. Brodie
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  2. L. D. Pearson
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  3. G. D. Snowder
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  4. J. C. DeMartini
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Brodie, S.J., Pearson, L.D., Snowder, G.D. et al. Host-virus interaction as defined by amplification of viral DNA and serology in lentivirus-infected sheep. Archives of Virology 130, 413–428 (1993). https://doi.org/10.1007/BF01309670

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  • DOI: https://doi.org/10.1007/BF01309670

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