Summary
The possibilities of PCR-technique for rapid influenza virus A diagnostic using universal and M- or NS- gene specific primers were demonstrated. The results of PCR-analyses of specimens from people correlated with virological, serological and immunochemical data and the results of spot-hybridization test.
References
Bechtereva T, Pavlov Y, Kramerov V, et al (1989) DNA sequencing with thermostable Tet DNA polymerase fromThermus thermophilus. Nucleic Acids Res 17: 10507
Buonagurio DA, Nakada S, Parvin JD, et al (1986) Evolution of human influenza A viruses over 50 years: rapid, uniform rate of change in NS gene. Science 232: 980–982
Gultyaev A (1991) The computer simulation of RNA folding involving pseudoknot formation. Nucleic Acids Res 19: 2489–2493
Erlich HA (ed) (1989) PCR technology. Principles and applications for DNA amplification. Stockton Press, New York
Havličkova M, Plyusnin A, Tumova B (1990) Influenza virus detection in clinical specimens. Acta Virol 34: 446–456
Katz JM, Wang M, Webster RG (1990) Direct sequencing of the HA gene of influenza (H 3N 2) virus in original clinical samples reveals sequence identity with mammalian cell-grown virus. J Virol 64: 1808–1811
Lucas WT, Whitaker-Dowling P, Kaifer CR, Younger JS (1988) Characterization of a unique protein produced by influenza A virus recovered from a long-term persistent infection. Virology 166: 620–623
Ortin J, Martinez C, Del Rio L, et al (1983) Evolution of the nucleotide sequence of influenza virus RNA segment 7 during drift of the H 3N 2 subtype. Gene 23: 233–239
Plyusnin A, Rozhkova S, Nolandt O, et al (1987) Molecular hybridization with DNA-probes as a laboratory diagnostic test for influenza viruses. Rev Roum Med Virol 38: 111–114
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113: 237–246
Robertson JS, Bootman JS, Newman R, et al (1987) Structural changes in the haemagglutinin which accompany egg adaptation of an influenza A(H 1N 1) virus. Virology 160: 31–37
Robertson JS, Bootman JS, Nicolson C, et al (1990) The haemagglutinin of influenza B virus present in clinical material is a single species identical to that of mammalian cell-grown virus. Virology 179: 35–40
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–511
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Pisareva, M., Bechtereva, T., Plyusnin, A. et al. PCR-amplification of influenza A virus specific sequences. Archives of Virology 125, 313–318 (1992). https://doi.org/10.1007/BF01309648
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DOI: https://doi.org/10.1007/BF01309648