Skip to main content

Advertisement

SpringerLink
Log in

The synergistic neutralization of Rift Valley fever virus by monoclonal antibodies to the envelope glycoproteins

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

A panel of monoclonal antibodies (MAbs) mapping to different antigenic sites on the RVFV G 1 and G 2 proteins were used to examine the mechanisms involved in neutralization of the virus. Three types of synergistic neutralization of RVFV were observed on mixing various pairs of MAbs. Firstly, enhanced neutralization occurred for two MAb pairs that showed augmented binding for G 2. These comprised a combination of a neutralizing MAb with a non-neutralizing antibody, as well as two antibodies which were non-neutralizing individually. In the second category, synergistic neutralization was observed between combinations of MAbs for which increased binding had not been detected. Lastly, mixtures of G 1 and G 2-specific MAbs were also capable of enhancing neutralization. Post-adsorption neutralization assays revealed that some MAbs neutralized cell-attached virus efficiently, indicating that they can neutralize by inhibiting the infection process after virus attachment. MAbs mapping to G 1 II e, G 2 I b and G 2 I c were unable to neutralize adsorbed virus and thus probably neutralize by preventing virus attachment to cells. Several G 1-reactive MAbs displayed low level post-adsorption activity, suggesting they may be capable of inhibiting RVFV infectivity at different stages of the replication cycle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Besselaar TG, Blackburn NK (1988) Antigenic analysis of West Nile virus strains using monoclonal antibodies. Arch Virol 99: 75–88

    Google Scholar 

  2. Besselaar TG, Blackburn NK (1991) Topological mapping of antigenic sites on the Rift Valley fever virus envelope glycoproteins using monoclonal antibodies. Arch Virol 121: 111–124

    Google Scholar 

  3. Bishop DHL, Calisher CH, Casals J, Chumakov MP, Gaidamovich SY, Hannoun C, Lvov DK, Marshall ID, Oker-Blom NM, Petterson RF, Porterfield JS, Russell PK, Shope RE, Westaway EG (1980) Bunyaviridae. Intervirology 14: 125–143

    Google Scholar 

  4. Choppin PW, Scheid A (1980) The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Rev Infect Dis 2: 40–61

    Google Scholar 

  5. Collett MS, Purchio AF, Keegan K, Frazier S, Hays W, Anderson DK, Parker MD, Schmaljohn C, Schmidt J, Dalrymple JM (1985) Complete nucleotide sequence of the M RNA segment of Rift Valley fever virus. Virology 144: 228–245

    Google Scholar 

  6. Collett MS, Keegan K, Hu SL, Sridhar P, Purchio AF, Ennis WH, Dalrymple JM (1987) Protective subunit immunogens to Rift Valley fever virus from bacteria and recombinant vaccina virus. In: Mahy BWJ, Kolakofshy D (eds) The biology of negative strand viruses. Elsevier, Amsterdam, pp 321–329

    Google Scholar 

  7. Dalrymple JM, Hasty SE, Kakach LT, Collett MS (1989) Mapping protective determinants of Rift Valley fever virus using recombinant vaccina viruses. In: Lerner RA, Ginsberg H, Chanock R, Brown F (eds) Vaccines ’89. Cold Spring Harbor Laboratory, New York, pp 371–375

    Google Scholar 

  8. Dietzschold BH, Tollis M, Lafon M, Wunner WH, Koprowski H (1987) Mechanisms of rabies virus neutralization by glycoprotein-specific monoclonal antibodies. Virology 161: 29–36

    Google Scholar 

  9. Dimmock NJ (1984) Mechanisms of neutralization of animal viruses. Review article. J Gen Virol 65: 1015–1022

    Google Scholar 

  10. Dimmock NJ (1987) Multiple mechanisms of neutralization of animal viruses. Trends Biochem Sci 12: 70–75

    Google Scholar 

  11. Emini EA, Kao S, Lewis AJ, Crainic R, Wimmer E (1983) Functional basis of poliovirus neutralization determined with monospecific antibodies. J Virol 46: 466–474

    Google Scholar 

  12. Highlander SL, Sutherland SL, Gage PJ, Johnson DC, Levine M, Glorioso JC (1987) Neutralizing monoclonal antibodies specific for herpes simplex virus glycoprotein D inhibit virus penetration. J Virol 61: 3356–3364

    Google Scholar 

  13. Iorio RM (1988) Mechanisms of neutralization of animal viruses — monoclonal antibodies provide a new perspective. Microbiol Pathogenesis 5: 1–7

    Google Scholar 

  14. Kingsford L (1984) Enhanced neutralization of La Crosse virus by the binding of specific pairs of monoclonal antibodies to the G 1 glycoprotein. Virology 136: 265–273

    Google Scholar 

  15. Laughlin LW, Meegan JM, Strausbaugh LJ, Morens DM, Watten RH (1979) Epidemic of Rift Valley fever virus in Egypt: observations of the spectrum of human illness. Trans Roy Soc Trop Med Hyg 73: 630–633

    Google Scholar 

  16. Lussenhop NO, Goertz R, Wakube-Bunoti M, Gehrz R, Kari B (1988) Epitope analysis of human cytomegalovirus glycoprotein complexes using murine monoclonal antibodies. Virology 164: 362–372

    Google Scholar 

  17. McCullough KC (1986) Monoclonal antibodies: implications for virology. Brief review. Arch Virol 87: 1–36

    Google Scholar 

  18. Oldstone MBA, Cooper NR, Larson DL (1974) Formation and biologic role of polyoma virus-antibody complexes — a critical role for complement. J Exp Med 140: 549–562

    Google Scholar 

  19. Saluzzo JF, Smith JF (1990) Use of reassortment viruses to map attenuating and temperature-sensitive mutations of the Rift Valley fever virus MP-12 vaccine. Vaccine 8: 369–375

    Google Scholar 

  20. Sarmiento M, Haffey M, Spear PG (1979) Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP 7 (B2) in virion infectivity. J Virol 239: 1149–1158

    Google Scholar 

  21. Schmaljohn CS, Parker MD, Ennis WH, Dalrymple JM, Collett MS, Suzich JA, Schmaljohn AL (1989) Baculovirus expression of the M segment of Rift Valley fever virus and examination of antigenic and immunogenic properties of the expressed proteins. Virology 170: 184–192

    Google Scholar 

  22. Shope RE, Peters CJ, Davies FG (1982) The spread of Rift Valley fever and approaches to its control. Bull WHO 60: 299–304

    Google Scholar 

  23. Taylor HP, Dimmock NJ (1985) Mechanisms of neutralization of influenza virus by IgM. J Gen Virol 66: 903–907

    Google Scholar 

  24. Van Drunen Littel-van-den Hurk S, Van den Hurk J, Babink LA (1985) Topographical analysis of bovine herpesvirus type-1 glycoproteins: use of monoclonal antibodies to identify and characterize functional epitopes. Virology 144: 216–227

    Google Scholar 

  25. Van Velden DJJ, Meyer JD, Olivier J, Gear JHS, McIntosh B (1977) Rift Valley fever affecting humans in South Africa. A clinico-pathological study. S Afr Med J 51: 867–871

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Virology, National Institute for Virology, University of the Witwatersrand, Sandringham, South Africa

    T. G. Besselaar & N. K. Blackburn

Authors
  1. T. G. Besselaar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. K. Blackburn
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Besselaar, T.G., Blackburn, N.K. The synergistic neutralization of Rift Valley fever virus by monoclonal antibodies to the envelope glycoproteins. Archives of Virology 125, 239–250 (1992). https://doi.org/10.1007/BF01309641

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01309641

Keywords

Search

Navigation