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Transient expression of the coat protein of sugarcane mosaic virus in sugarcane protoplasts and expression inEscherichia coli

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Summary

The coat protein (CP) of strain SC of sugarcane mosaic virus (SCMV-SC) was expressed transiently in sugarcane protoplasts after electroporation with one of two plasmids encoding the CP gene. The CP gene was fused with either the cauliflower mosaic virus 35S promoter or the synthetic monocotyledon promoter “Emu”. The coat protein gene was also inducibly expressed inEscherichia coli when fused to the trc promoter. The protein expressed in both systems had the same electrophoretic mobility and antigenic specificity as purified SCMV-SC coat protein. Transient expression of the 35S-CP gene in protoplasts could only be demonstrated in Western blots developed with the chemiluminescence enzyme substrate luminol.

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Authors and Affiliations

  1. Bureau of Sugar Experiment Stations, Indooroopilly, Queensland, Australia

    G. R. Smith & R. Ford

  2. Division of Biomolecular Engineering, CSIRO, Parkville, Victoria, Australia

    M. J. Frenkel & D. D. Shukla

  3. Centre for Molecular Biotechnology, Queensland University of Technology, Brisbane, Queensland, Australia

    J. L. Dale

Authors
  1. G. R. Smith
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  2. R. Ford
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  3. M. J. Frenkel
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  4. D. D. Shukla
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  5. J. L. Dale
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Smith, G.R., Ford, R., Frenkel, M.J. et al. Transient expression of the coat protein of sugarcane mosaic virus in sugarcane protoplasts and expression inEscherichia coli . Archives of Virology 125, 15–23 (1992). https://doi.org/10.1007/BF01309625

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  • DOI: https://doi.org/10.1007/BF01309625

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