Skip to main content
SpringerLink
Log in

Characterization of a prolate-headed bacteriophage ofLactobacillus delbrueckii subsp.lactis, and its DNA homology with isometric-headed phages

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

A newLactobacillus delbrueckii subsp.lactis bacteriophage, JCL 1032, was characterized. JCL 1032 had a small, elongated prolate head, and a long non-contractile tail with cross-bars. The restriction map of JCL 1032 genome was constructed with five endonucleases. The genome was 45.8 kb in size, and it had cohesive ends (cos). Molecular masses of the phage structural proteins were also determined. JCL 1032 showed DNA homology with morphologically dissimilar, isometric-headed phages ofLb. delbrueckii (subsp.lactis and subsp.bulgaricus) when analyzed by Southern hybridization. Although in general JCL 1032 was only distantly related to isometric-headed phages, there were also a few short highly homologous (minimal homology 84%) DNA regions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Accolas J-P, Spillmann H (1979) Morphology of bacteriophages ofLactobacillus bulgaricus, L. lactis andL. helveticus. J Appl Bacteriol 47: 309–319

    Google Scholar 

  2. Ackermann H-W, Cantor ED, Jarvis AW, Lembke J, Mayo J (1984) New species definitions in phages of gram-positive cocci. Intervirology 22: 181–190

    Google Scholar 

  3. Alatossava T, Pyhtilä MJ (1980) Characterization of a newLactobacillus lactis bacteriophage. IRCS Med Sci 8: 297–298

    Google Scholar 

  4. Alatossava T, Forsman P, Mikkonen M, Vasala A (1991) Molecular biology ofLactobacillus phage LL-H. Finn J Dairy Sci 49: 1–13

    Google Scholar 

  5. Bergquist PL (1987) Incompatibility. In: Hardy KG (ed) Plasmids: a practical approach. IRL Press, Oxford, pp 37–38

    Google Scholar 

  6. Botstein D (1980) A theory of modular evolution for bacteriophages. Ann N Y Acad Sci 354: 484–490

    Google Scholar 

  7. Coveney JA, Fitzgerald GF, Daly C (1987) Detailed characterization and comparison of four lactic streptococcal bacteriophages based on morphology, restriction mapping, DNA homology, and structural protein analysis. Appl Environ Microbiol 53: 1439–1447

    Google Scholar 

  8. Forsman P, Alatossava T (1991) Genetic variation ofLactobacillus delbrueckii subsp.lactis bacteriophages isolated from cheese processing plants in Finland. Appl Environ Microbiol 57: 1805–1812

    Google Scholar 

  9. Francki RIB, Fauquet CM, Knudson DL, Brown F (eds) (1991) Classification and nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Springer-Verlag, Wien New York (Arch Virol [Suppl] 2)

    Google Scholar 

  10. Hendrix RW, Roberts JW, Stahl FW, Weisberg RA (1983) Lambda II. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  11. Jarvis AW (1984) Differentiation of lactic streptococcal phages into phage species by DNA-DNA homology. Appl Environ Microbiol 47: 343–349

    Google Scholar 

  12. Jarvis AW (1989) Bacteriophages of lactic acid bacteria. J Dairy Sci 72: 3406–3428

    Google Scholar 

  13. Jarvis AW, Fitzgerald GF, Mata M, Mercenier A, Neve H, Powell IB, Ronda C, Saxelin M, Teuber M (1991) Species and type phages of lactococcal bacteriophages. Intervirology 32: 2–9

    Google Scholar 

  14. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680–685

    Google Scholar 

  15. Lahbib-Mansais Y, Mata M, Ritzenthaler P (1988) Molecular taxonomy ofLactobacillus phages. Biochimie 70: 429–435

    Google Scholar 

  16. Mata M, Trautwetter A, Luthaud G, Ritzenthaler P (1986) Thirteen virulent and temperate bacteriophages ofLactobacillus bulgaricus andLactobacillus lactis belong to a single DNA homology group. Appl Environ Microbiol 52: 812–818

    Google Scholar 

  17. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  18. Sechaud L, Cluzel P-J, Rousseau M, Baumgartner A, Accolas J-P (1988) Bacteriophages of lactobacilli. Biochimie 70: 401–410

    Google Scholar 

  19. Shearman CA, Hertwig S, Teuber M, Gasson MJ (1991) Characterization of the prolate-headed lactococcal bacteriophage ϕvML 3: location of the lysin gene and its DNA homology with other prolate-headed phages. J Gen Microbiol 137: 1285–1291

    Google Scholar 

  20. Shimizu-Kadota M, Sakurai T, Tsuchida N (1983) Prophage origin of a virulent phage appearing on fermentations ofLactobacillus casei S-1. Appl Environ Microbiol 45: 669–674

    Google Scholar 

  21. Tohyama K, Sakurai T, Arai H, Oda A (1972) Studies on temperate phages ofLactobacillus salivarius. I. Morphological, biological and serological properties of newly isolated temperate phages ofLactobacillus salivarius. Jpn J Microbiol 16: 385–395

    Google Scholar 

  22. Towbin HT, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354

    Google Scholar 

  23. Trautwetter A, Ritzenthaler P, Alatossava T, Mata-Gilsinger M (1986) Physical and genetic characterization of the genome ofLactobacillus lactis bacteriophage LL-H. J Virol 59: 551–555

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, University of Oulu, Oulu, Finland

    P. Forsman

Authors
  1. P. Forsman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Forsman, P. Characterization of a prolate-headed bacteriophage ofLactobacillus delbrueckii subsp.lactis, and its DNA homology with isometric-headed phages. Archives of Virology 132, 321–330 (1993). https://doi.org/10.1007/BF01309542

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01309542

Keywords

Search

Navigation