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Monoclonal antibodies against BLV-transformed sheep cells

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Summary

Hybrid cell lines were prepared by the fusion of BALB/c myeloma, P3X63Ag 8.653 cells with the spleen cells of BALB/c mice that were immunized with SF-28 cells, sheep fibroblasts transformed with BLVin vitro. Five clones of hybrid progeny produced cytotoxic IgG1 antibodies against SF-28 cells. The tested monoclonal antibodies showed cytotoxic activity against 4 out of 6 BLV-transformed sheep fibroblasts as well as SF-28 cells but failed to react with other cells, including BLV-producing FLK cells, bovine lymphosarcomas and normal sheep cells. These monoclonal antibodies showed suppressive effect on colony formation of SF-28 cells in soft agar culture.

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Authors and Affiliations

  1. Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan

    K. Hamada, M. Onuma, K. Tsukiyama, T. Mikami & H. Izawa

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  1. K. Hamada
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  2. M. Onuma
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  3. K. Tsukiyama
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  4. T. Mikami
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  5. H. Izawa
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Hamada, K., Onuma, M., Tsukiyama, K. et al. Monoclonal antibodies against BLV-transformed sheep cells. Archives of Virology 81, 171–175 (1984). https://doi.org/10.1007/BF01309306

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  • DOI: https://doi.org/10.1007/BF01309306

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