Summary
Mumps virus replication was examined in various culture cells derived from mice. Eight of 16 lymphoid cell lines and 4 of 13 non-lymphoid cell lines supported the replication of Vero cell-adapted Enders strain (EY) of mumps virus. EY strain replicated more efficiently in lymphoid cell lines than in non-lymphoid ones. T cell preference, however, was not observed in this study.
The growth kinetics of EY strain in high yield cell lines such as EL-4, L1210 and NS-18 cells were similar to that in Vero cells, while in low yield cell lines such as DK, C243 and 203GL cells the growth patterns varied respectively.
Nineteen kinds of primary culture cells of murine origin all proved not to be susceptible for EY strain, even when spleen cells were stimulated with lectins or allogeneic cells.
Seven other strains of mumps virus were examined for their ability to replicate in EL-4, L1210 and L929 cells. Four and 6 strains replicated in EL-4 cells and L1210 cells respectively, although the growth patterns and yields varied in each virus-cell combinations. On the other hand, none of 7 strains showed sufficient replication in L929 cells.
Similar content being viewed by others
References
Bolin, V. S., Anderson, J. A., Leymaster, G. R.: Experimental intraocular infection of guinea pigs with mumps virus. Proc. Soc. Exp. Biol. Med.75, 166–170 (1950).
Cantell, K.: Mumps virus. In:Kenneth, M. S., Lauffer, M. A. (eds.), Advances in virus research, Vol. 8, 123–164. New York: Academic Press 1961.
Chantler, J. K., Tingle, A. J.: Replication and expression of rubella virus in human lymphocyte populations. J. gen. Virol.50, 317–328 (1980).
Davis, L. E., Johnson, R. T.: Experimental viral infections of the inner ear. I. Acute infections of the newborn hamster labyrinth. Lab. Invest.34, 349–356 (1976).
Feldman, H. A.: Mumps. In:Evans, A. S. (ed.), Viral infections of humans: Epidemiology and control, 419–440. New York: Plenum Press 1982.
Fleischer, B., Kreth, H. W.: Mumps virus replication in human lymphoid cell lines and in peripheral blood lymphocytes: Preference for T cells. Infect. Immun.35, 25–31 (1982).
Gerhard, W.: Fusion of cells in suspension and outgrowth of hybrids in conditioned medium. In:Kennett, R. H., Mckearn, T. J., Bechtol, K. B. (eds.), Monoclonal antibodies. Hybridomas: A new dimension in biological analysis, 370–371. New York: Plenum Press 1980.
Herrler, G., Compans, R. W.: Synthesis of mumps virus in infected Vero cells. Virology119, 430–438 (1982).
Homma, M., Ohuchi, M.: Trypsin action on the growth of Sendai virus in tissue culture cells III. Structural difference of Sendai viruses grown in eggs and tissue culture cells. J. Virol.12, 1457–1465 (1973).
Kilham, L., Murphy, H. W.: Propagation of mumps virus in suckling mice and mouse embryo tissue cultures. Proc. Soc. Exp. Biol. Med.80, 495–498 (1952).
Kilham, L., Overman, J. R.: Natural pathogenicity of mumps virus for suckling hamsters on intracerebral inoculation. J. Immunol.70, 147–151 (1953).
Nagai, Y., Ito, Y., Hamaguchi, M., Yoshida, T., Matsumoto, T.: Relation of interferon production to the limited replication of Neweastle disease virus in L cells. J. gen. Virol.55, 109–116 (1981).
Nagata, I., Maeno, K., Yoshii, S., Matsumoto, T.: Plaque formation by HVJ in calf kidney cells. Arch. ges. Virusforsch.15, 257–259 (1965).
Naruse, H., Nagai, Y., Yoshida, T., Hamaguchi, M., Matsumoto, T., Isomura, S., Suzuki, S.: The polypeptides of mumps virus and their synthesis in infected chick embryo cells. Virology112, 119–130 (1981).
Sullivan, J. L., Barry, D. W., Lucas, S. J., Albrecht, P.: Measles infection of human mononuclear cells. I. Acute infection of peripheral blood lymphocytes and monocytes. J. Exp. Med.142, 773–784 (1975).
Wolinsky, J. S., Klassen, T., Baringer, J. R.: Persistence of neuroadapted mumps virus in brains of newborn hamsters after intraperitoneal inoculation. J. Infect. Dis.133, 260–267 (1976).
Wolinsky, J. S., Streep, W. G.: Virulence and persistence of three prototype strains of mumps virus in newborn hamsters. Arch. Virol.57, 355–359 (1978).
Yamada, A., Taguchi, F., Fujiwara, K.: T Lymphocyte-dependent difference in susceptibility between DDD and C3H mice to mouse hepatitis virus. Japan. J. Exp. Med.49, 413–421 (1979).
Author information
Authors and Affiliations
Additional information
With 2 Figures
Rights and permissions
About this article
Cite this article
Tsurudome, M., Yamada, A., Hishiyama, M. et al. Replication of mumps virus in murine cells. Archives of Virology 81, 13–24 (1984). https://doi.org/10.1007/BF01309293
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309293