Summary
19F nuclear magnetic resonance (NMR) imaging and19F NMR chemical-shift imaging (19F CSI) have been used to localize fluorinated compounds administered to stems ofAncistrocladus heyneanus andA. abbreviatus for the elucidation of biosynthetic pathways in living plants. This first application of19F CSI on plants proved CSI to be a valuable technique for mapping fluorinated molecules in plants. Exemplarily using trifluoroacetate as a model compound allowed to select appropriate feeding methods and to optimize both concentration and duration of the application to the plant. The time course of the uptake and distribution of trifluoroacetate was monitored by both19F imaging and19F CSI. Fluorinated metabolites formed by uptake of 3-fluoro-3-deoxy-D-glucose were detected with19F CSI.
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Abbreviations
- 3-FDG:
-
3-fluoro-3-deoxy-D-glucose
- CSI:
-
chemicalshift imaging
- NMR:
-
nuclear magnetic resonance
- SNR:
-
signal-to-noise ratio
- TFA:
-
trifluoroacetate
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Dedicated to Professor Manfred Christi on the occasion of his 60th birthday
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Bringmann, G., Wolf, K., Meininger, M. et al. In vivo19F NMR chemical-shift imaging ofAncistrocladus species. Protoplasma 218, 134–143 (2001). https://doi.org/10.1007/BF01306603
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DOI: https://doi.org/10.1007/BF01306603