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Correlation of trypsin-plasma inhibitor complexes with mortality in experimental pancreatitis in rats

Digestive Diseases and Sciences volume 31, pages 961–969 (1986)Cite this article

Abstract

Activation of trypsinogen in acute pancreatitis results in subsequent increases in plasma levels of trypsin bound to the inhibitors α1-protease inhibitor (α1-PI) and α-macroglobulin (α-M). It seems logical to speculate that plasma levels of these inhibitor-bound forms of trypsin may reflect the degree of intrapancreatic zymogen activation and that determination of such parameters may be of diagnostic and prognostic value. In order to test this hypothesis, the concentrations of trypsinogen and of trypsin bound to α1-PI have been determined in serial plasma samples from rats who died (N=7) and survived (N=5) following induction of pancreatitis with taurocholate. Since the other major reaction product of active trypsin in plasma, α-macroglobulin-bound trypsin, cannot be measured directly, the plasma levels of trypsin-like amidase activity were determined to estimate the concentration of trypsin-α-M complex. Shortly after induction of pancreatitis, elevated levels of trypsinogen were present in plasma, but no α1-PI-bound trypsin could be detected. Trypsin-α1-PI complex continuously increased over the time course of pancreatitis in animals that died. In contrast, the plasma levels of trypsin-α1-PI complex were lower in animals that survived, peaked around 15 hr postinduction at levels (182±53 ng/ml) significantly lower than those in dying animals (543±346 ng/ml), and fell during the following 48 hr. There was a significant correlation between plasma trypsin-like amidase activity and plasma α1-PI-bound trypsin. Our data demonstrate that the concentration of activated forms of plasma trypsin in the bloodstream are correlated with mortality in experimental pancreatitis.

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Author information

Author notes

  1. R. D. Reidelberger PhD

    Present address: Harry S. Truman G. I. Research Laboratory, VA Medical Center, 65201, Columbia, Missouri

Affiliations

  1. Research Room 108, Biochemistry Research Laboratory, Martinez VA Medical Center, 150 Muir Road, 94553, Martinez, California

    C. Largman PhD, R. D. Reidelberger PhD & H. Tsukamoto DVM

  2. Departments of Internal Medicine and Biological Chemistry, UC Davis School of Medicine, 95616, Davis, California

    C. Largman PhD, R. D. Reidelberger PhD & H. Tsukamoto DVM

Authors
  1. C. Largman PhD
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  2. R. D. Reidelberger PhD
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  3. H. Tsukamoto DVM
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Additional information

This work was supported by the Research Service of the Veterans Administration and by an NIH grant (HL25408) to C.L.

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Largman, C., Reidelberger, R.D. & Tsukamoto, H. Correlation of trypsin-plasma inhibitor complexes with mortality in experimental pancreatitis in rats. Digest Dis Sci 31, 961–969 (1986). https://doi.org/10.1007/BF01303217

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  • DOI: https://doi.org/10.1007/BF01303217

Keywords

  • Public Health
  • Pancreatitis
  • Plasma Level
  • Trypsin
  • Elevated Level