Skip to main content

Gastric acid secretion and lesion formation in rats under water-immersion stress


In attempts to investigate the roles of acid in the pathogenesis of stress-induced gastric lesions, gastric acid secretion was studied in pylorus-ligated and lumen-perfused rats under restraint alone (R) or restraint with additional water immersion (WI). Gastric mucosal blood flow (GMBF) was measured with the aminopyrine clearance method in acute fistula rats. Acid secretion in pylorus-ligated rats significantly decreased under R or WI of either 3.5 or 7 hr stress. In the lumen-perfused or acute-fistula rats, exposure of rats to stress for 7 hr produced a similar decrease; however, in the WI group, there was a significant increase of acid secretion for 3–4 hr during stress, but not exceeding the prestress level. Only in the WI group did GMBF exhibit similar increases to those of acid secretory activity, and these increases were significantly inhibited by intraperitoneal administration of atropine (1mg/kg) or cimetidine (60mg/kg). Gastric lesions developed in both groups at 3.5hr and became extensively severe at 7hr only in the WI group. Cimetidine failed to influence the formation of lesions at 3.5hr but significantly inhibited the later outgrowth of lesions at 7hr, while atropine or pylorus ligation all but completely prevented lesions induced by either 3.5-or 7-hr WI stress. These results indicate that exposure of rats to stress (R or WI) generally decreased acid secretory activity, but there was a rise in acid secretion toward normal levels during WI stress, which may play an important role in the aggravating process of stress-induced gastric lesions.

This is a preview of subscription content, access via your institution.


  1. Menguy R: Effects of restraint stress on gastric secretion in the rat. Am J Dig Dis 5:911–916, 1960

    Article  PubMed  CAS  Google Scholar 

  2. Brodie DA, Marchall RW, Morena OM: Effects of restraint on gastric acidity in the rat. Am J Physiol 202:812–814, 1964

    Google Scholar 

  3. Takeuchi K, Okabe S, Takagi K: A new model of stress ulcers in the rat with pylorus ligation and its pathogenesis. Am J Dig Dis 21:782–788, 1976

    Article  PubMed  CAS  Google Scholar 

  4. Hase T, Anderson PR, Mehlman B: Significance of gastric secretory changes in the pathogenesis of stress ulcers. Am J Dig Dis 20:443–449, 1975

    Article  PubMed  CAS  Google Scholar 

  5. Kitagawa H, Fujiwara M, Osumi Y: Effect of water-immersion stress on gastric secretion and mucosal blood flow in rats. Gastroenterology 77:298–302, 1979

    PubMed  CAS  Google Scholar 

  6. Jacobson ED, Linford RH, Grossman MI: Gastric secretion in relation to mucosal blood flow studied by a clearance technique. J Clin Invest 45:1–13, 1966

    Article  PubMed  CAS  Google Scholar 

  7. Brodie BB, Axelrod J: The fate of aminopyrine (pyramidon) in man and methods for the estimation of aminopyrine and its metabolites in biological materials. J Pharmacol Exp Ther 99:171–174, 1950

    PubMed  CAS  Google Scholar 

  8. Wolf U, Schwill PO, Englehardt W: Gastric secretion, mucosal erosion and porto-systemic gastrin gradients as induced by different degrees of stress in the rat. Res Exp Med 179:229–237, 1981

    Article  CAS  Google Scholar 

  9. Leung FW, Guth PH, Scremin OU, Golanska EM, Kauffman GL: Regional gastric mucosal blood flow measurements by hydrogen gas clearance in the anesthetized rat and rabbit. Gastroenterology 87:28–36, 1984

    PubMed  CAS  Google Scholar 

  10. Selye H: Geheral adaptation syndrome and diseases of adaptation. J Clin Endocrinol Metab 6:117–230, 1946

    Article  CAS  Google Scholar 

  11. Dai S, Ogle LW: Gastric ulcers induced by acid accumulation and by stress in pylorus-occluded rats. Eur J Pharmacol 26:15–21, 1974

    Article  PubMed  CAS  Google Scholar 

  12. Takagi K, Kasuya Y, Watanabe K: Studies on the drugs for peptic ulcers: A reliable method for producing stress ulcers in rats. Chem Pharm Bull (Tokyo) 12:465–472, 1964

    CAS  Google Scholar 

  13. Yano S, Akahane M, Harada M: Role of gastric motility in develpment of stress-induced gastric lesions of rats. Jpn J Pharmacol 28:607–615, 1978

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hayase, M., Takeuchi, K. Gastric acid secretion and lesion formation in rats under water-immersion stress. Digest Dis Sci 31, 166–171 (1986).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: